Abstract
Shading is a widely applied strategy to increase theanine and chlorophyll levels in tea leaves during matcha production. However, the regulatory mechanism of theanine accumulation under shading conditions remains elusive. Through visualization analysis of PIF genes under shading conditions, we identified a homologue of AtPIF7 gene, CsPIF7, highly responsive to shading treatment. Through co-expression analysis, we found that CsPIF7 expression patterns exhibited a high correlation with those of the key theanine biosynthesis genes, CsTSⅠ and CsGSⅠ. Silencing of CsPIF7 significantly reduced the expression of CsTSⅠ and CsGSⅠ and theanine content. On the contrary, overexpression of CsPIF7 increased the expression levels of CsTSⅠ and CsGSⅠ and theanine content. Yeast one-hybrid (Y1H), dual-luciferase reporter assay and Electrophoretic Mobility Shift Assay (EMSA) further demonstrated that CsPIF7 could bind to the promoters of CsTSⅠ and CsGSⅠ, and activate the expression of CsTSⅠ and CsGSⅠ. In conclusion, CsPIF7 could mediate the shading-induced biosynthesis of theanine by directly binding to and activating the expression of CsTSⅠ and CsGSⅠ. This study provides new insights into molecular mechanisms for the production of greener and fresher matcha under shading conditions.
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Claims 9
Claim · model-written summary
CsPIF7 was highly responsive to shading.
- Taxon
- Tea Plants (Camellia sinensis)
Camellia sinensist0 - Gene
- CsPIF7e0
- Outcome
- highly responsive to shading treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tea Plants (Camellia sinensis)”
e0“CsPIF7, highly responsive to shading treatment”
Claim · model-written summary
CsPIF7 expression correlated with CsTSⅠ and CsGSⅠ expression.
- Taxon
- Tea Plants (Camellia sinensis)Camellia sinensist0
- Gene
- CsPIF7e0
- Outcome
- high correlatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tea Plants (Camellia sinensis)”
e0“CsPIF7 expression patterns exhibited a high correlation with those of the key theanine biosynthesis genes, CsTSⅠ and CsGSⅠ”
Claim · model-written summary
CsPIF7 silencing significantly reduced CsTSⅠ and CsGSⅠ expression.
- Taxon
- Tea Plants (Camellia sinensis)Camellia sinensist0
- Treatment
- Silencing of CsPIF7e0
- Gene
- CsPIF7e0
- Outcome
- expression of CsTSⅠ and CsGSⅠe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tea Plants (Camellia sinensis)”
e0“Silencing of CsPIF7 significantly reduced the expression of CsTSⅠ and CsGSⅠ and theanine content”
Claim · model-written summary
CsPIF7 silencing significantly reduced theanine content.
- Taxon
- Tea Plants (Camellia sinensis)Camellia sinensist0
- Treatment
- Silencing of CsPIF7e0
- Gene
- CsPIF7e0
- Outcome
- theanine contente0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tea Plants (Camellia sinensis)”
e0“Silencing of CsPIF7 significantly reduced the expression of CsTSⅠ and CsGSⅠ and theanine content”
Claim · model-written summary
CsPIF7 overexpression increased CsTSⅠ and CsGSⅠ expression.
- Taxon
- Tea Plants (Camellia sinensis)Camellia sinensist0
- Treatment
- overexpression of CsPIF7e0
- Gene
- CsPIF7e0
- Outcome
- expression levels of CsTSⅠ and CsGSⅠe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tea Plants (Camellia sinensis)”
e0“overexpression of CsPIF7 increased the expression levels of CsTSⅠ and CsGSⅠ and theanine content”
Claim · model-written summary
CsPIF7 overexpression increased theanine content.
- Taxon
- Tea Plants (Camellia sinensis)Camellia sinensist0
- Treatment
- overexpression of CsPIF7e0
- Gene
- CsPIF7e0
- Outcome
- theanine contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tea Plants (Camellia sinensis)”
e0“overexpression of CsPIF7 increased the expression levels of CsTSⅠ and CsGSⅠ and theanine content”
Claim · model-written summary
CsPIF7 bound CsTSⅠ and CsGSⅠ promoters.
- Taxon
- Tea Plants (Camellia sinensis)Camellia sinensist0
- Gene
- CsPIF7e0
- Outcome
- bind to the promoters of CsTSⅠ and CsGSⅠe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tea Plants (Camellia sinensis)”
e0“CsPIF7 could bind to the promoters of CsTSⅠ and CsGSⅠ”
Claim · model-written summary
CsPIF7 activated CsTSⅠ and CsGSⅠ expression.
- Taxon
- Tea Plants (Camellia sinensis)Camellia sinensist0
- Gene
- CsPIF7e0
- Outcome
- expression of CsTSⅠ and CsGSⅠe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tea Plants (Camellia sinensis)”
e0“CsPIF7 could bind to the promoters of CsTSⅠ and CsGSⅠ, and activate the expression of CsTSⅠ and CsGSⅠ”
Claim · model-written summary
CsPIF7 mediates shading-induced theanine biosynthesis by directly binding and activating biosynthesis genes.
- Taxon
- Tea Plants (Camellia sinensis)Camellia sinensist0
- Gene
- CsPIF7e0
- Outcome
- shading-induced biosynthesis of theaninee0
- Qualifier
- directly binding to and activating the expression of CsTSⅠ and CsGSⅠe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tea Plants (Camellia sinensis)”
e0“CsPIF7 could mediate the shading-induced biosynthesis of theanine by directly binding to and activating the expression of CsTSⅠ and CsGSⅠ”
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See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.