Paper · 2025 · Egyptian Journal of Botany

Enhancing the Growth and Chemical Composition of Cardoon Plant by Fertilization with Azolla, Mycorrhizae, Chitosan, and EDTA-Zinc

Amaal A.M. Heikal; Rania Saber Yuosef

12
claims
25
verbatim quotes
0
citations
2
research areas
—
Not open access

Abstract

Due to the limitations and high cost of chemical fertilizers, in addition to their negative effects on medicinal plants—especially pharmaceutical ones—two-season field experiments were conducted in clay loam soil to evaluate the efficacy of azolla, mycorrhizae, chitosan, or EDTA-Zinc on the growth, oil content, and chemical analysis of Cynara cardunculus plants. Azolla, mycorrhizae, and chitosan were applied alone or in combination with EDTA-Zinc. The growth traits were enhanced by foliar application of chitosan in terms of the number of leaves, heads, and seeds per head or plant, as well as chlorophyll content, while fertilization with azolla powder plus EDTA-Zinc resulted in the maximum values of leaf length, flower stem length, fresh leaf weight, and seeds per plant, and significantly improved the oil yield per plant. The highest percent of oil (25.27% and 25.33%) was obtained from seeds of plants treated with mycorrhizae plus zinc in the two seasons. Additionally, the same treatment gave the highest levels of phenolic and flavonoid compounds in cardoon leaves. The major constituents of the fixed oil were linoleic acid, oleic acid, palmitic acid, and stearic acid. The highest amount of unsaturated fatty acids was found in azolla liquid and mycorrhizae treatments. Our findings indicated that the application of azolla, mycorrhizae, and chitosan can be used alone or as a complement to EDTA-Zinc to enhance growth, oil percentage, and oil yield in cardoon plants. Applying these biofertilizers had a significant effect by accelerating plant growth.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 12

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar application of chitosan enhanced the number of leaves, heads, and seeds per head or plant of cardoon.

Taxon
Cynara cardunculusCynara cardunculuse0
Treatment
foliar application of chitosane1
Outcome
number of leaves, heads, and seeds per head or plante1
Direction
enhancede1
Setting
two-season field experimentse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cynara cardunculus
Cynara cardunculus · TaxonBurkhard Mücke · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“two-season field experiments were conducted in clay loam soil to evaluate the efficacy of azolla, mycorrhizae, chitosan, or EDTA-Zinc on the growth, oil content, and chemical analysis of Cynara cardunculus plants”

✓ located verbatim in the source · abstract, characters 151–363 · show in text ↑
e1

“The growth traits were enhanced by foliar application of chitosan in terms of the number of leaves, heads, and seeds per head or plant, as well as chlorophyll content”

✓ located verbatim in the source · abstract, characters 452–618 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar application of chitosan enhanced chlorophyll content of cardoon.

Taxon
Cynara cardunculusCynara cardunculuse0
Treatment
foliar application of chitosane1
Outcome
chlorophyll contente1
Direction
enhancede1
Setting
two-season field experimentse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cynara cardunculus
Cynara cardunculus · TaxonBurkhard Mücke · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“two-season field experiments were conducted in clay loam soil to evaluate the efficacy of azolla, mycorrhizae, chitosan, or EDTA-Zinc on the growth, oil content, and chemical analysis of Cynara cardunculus plants”

✓ located verbatim in the source · abstract, characters 151–363 · show in text ↑
e1

“The growth traits were enhanced by foliar application of chitosan in terms of the number of leaves, heads, and seeds per head or plant, as well as chlorophyll content”

✓ located verbatim in the source · abstract, characters 452–618 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Fertilization with azolla powder plus EDTA-Zinc gave the maximum leaf length, flower stem length and fresh leaf weight of cardoon.

Taxon
Cynara cardunculusCynara cardunculuse0
Treatment
fertilization with azolla powder plus EDTA-Zince2
Outcome
leaf length, flower stem length, fresh leaf weighte2
Direction
maximume2
Setting
two-season field experimentse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cynara cardunculus
Cynara cardunculus · TaxonBurkhard Mücke · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“two-season field experiments were conducted in clay loam soil to evaluate the efficacy of azolla, mycorrhizae, chitosan, or EDTA-Zinc on the growth, oil content, and chemical analysis of Cynara cardunculus plants”

✓ located verbatim in the source · abstract, characters 151–363 · show in text ↑
e2

“while fertilization with azolla powder plus EDTA-Zinc resulted in the maximum values of leaf length, flower stem length, fresh leaf weight, and seeds per plant, and significantly improved the oil yield per plant”

✓ located verbatim in the source · abstract, characters 620–831 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Fertilization with azolla powder plus EDTA-Zinc gave the maximum number of seeds per plant of cardoon.

Taxon
Cynara cardunculusCynara cardunculuse0
Treatment
fertilization with azolla powder plus EDTA-Zince2
Outcome
seeds per plante2
Direction
maximume2
Setting
two-season field experimentse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cynara cardunculus
Cynara cardunculus · TaxonBurkhard Mücke · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“two-season field experiments were conducted in clay loam soil to evaluate the efficacy of azolla, mycorrhizae, chitosan, or EDTA-Zinc on the growth, oil content, and chemical analysis of Cynara cardunculus plants”

✓ located verbatim in the source · abstract, characters 151–363 · show in text ↑
e2

“while fertilization with azolla powder plus EDTA-Zinc resulted in the maximum values of leaf length, flower stem length, fresh leaf weight, and seeds per plant, and significantly improved the oil yield per plant”

✓ located verbatim in the source · abstract, characters 620–831 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Fertilization with azolla powder plus EDTA-Zinc significantly improved the oil yield per plant of cardoon.

Taxon
Cynara cardunculusCynara cardunculuse0
Treatment
fertilization with azolla powder plus EDTA-Zince2
Outcome
oil yield per plante2
Direction
improvede2
Setting
two-season field experimentse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cynara cardunculus
Cynara cardunculus · TaxonBurkhard Mücke · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“two-season field experiments were conducted in clay loam soil to evaluate the efficacy of azolla, mycorrhizae, chitosan, or EDTA-Zinc on the growth, oil content, and chemical analysis of Cynara cardunculus plants”

✓ located verbatim in the source · abstract, characters 151–363 · show in text ↑
e2

“while fertilization with azolla powder plus EDTA-Zinc resulted in the maximum values of leaf length, flower stem length, fresh leaf weight, and seeds per plant, and significantly improved the oil yield per plant”

✓ located verbatim in the source · abstract, characters 620–831 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Mycorrhizae plus zinc gave the highest seed oil percentage of cardoon, 25.27% and 25.33% in the two seasons.

Taxon
Cynara cardunculusCynara cardunculuse0
Organ / stage
seedse3
Treatment
mycorrhizae plus zince3
Outcome
percent of oile3
Direction
higheste3
Value
25.27% and 25.33%e3
Setting
two-season field experimentse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cynara cardunculus
Cynara cardunculus · TaxonBurkhard Mücke · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“two-season field experiments were conducted in clay loam soil to evaluate the efficacy of azolla, mycorrhizae, chitosan, or EDTA-Zinc on the growth, oil content, and chemical analysis of Cynara cardunculus plants”

✓ located verbatim in the source · abstract, characters 151–363 · show in text ↑
e3

“The highest percent of oil (25.27% and 25.33%) was obtained from seeds of plants treated with mycorrhizae plus zinc in the two seasons”

✓ located verbatim in the source · abstract, characters 833–967 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Mycorrhizae plus zinc gave the highest levels of phenolic and flavonoid compounds in cardoon leaves.

Taxon
Cynara cardunculusCynara cardunculuse0
Organ / stage
leavese4
Treatment
mycorrhizae plus zince3
Outcome
levels of phenolic and flavonoid compoundse4
Direction
higheste4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cynara cardunculus
Cynara cardunculus · TaxonBurkhard Mücke · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“two-season field experiments were conducted in clay loam soil to evaluate the efficacy of azolla, mycorrhizae, chitosan, or EDTA-Zinc on the growth, oil content, and chemical analysis of Cynara cardunculus plants”

✓ located verbatim in the source · abstract, characters 151–363 · show in text ↑
e3

“The highest percent of oil (25.27% and 25.33%) was obtained from seeds of plants treated with mycorrhizae plus zinc in the two seasons”

✓ located verbatim in the source · abstract, characters 833–967 · show in text ↑
e4

“Additionally, the same treatment gave the highest levels of phenolic and flavonoid compounds in cardoon leaves”

✓ located verbatim in the source · abstract, characters 969–1079 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The major constituents of the fixed oil of cardoon were linoleic acid, oleic acid, palmitic acid and stearic acid.

Taxon
Cynara cardunculusCynara cardunculuse0
Outcome
major constituents of the fixed oile5
Qualifier
linoleic acid, oleic acid, palmitic acid, and stearic acide5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cynara cardunculus
Cynara cardunculus · TaxonBurkhard Mücke · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“two-season field experiments were conducted in clay loam soil to evaluate the efficacy of azolla, mycorrhizae, chitosan, or EDTA-Zinc on the growth, oil content, and chemical analysis of Cynara cardunculus plants”

✓ located verbatim in the source · abstract, characters 151–363 · show in text ↑
e5

“The major constituents of the fixed oil were linoleic acid, oleic acid, palmitic acid, and stearic acid”

✓ located verbatim in the source · abstract, characters 1081–1184 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Azolla liquid gave the highest amount of unsaturated fatty acids in cardoon oil.

Taxon
Cynara cardunculusCynara cardunculuse0
Treatment
azolla liquide6
Outcome
amount of unsaturated fatty acidse6
Direction
higheste6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cynara cardunculus
Cynara cardunculus · TaxonBurkhard Mücke · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“two-season field experiments were conducted in clay loam soil to evaluate the efficacy of azolla, mycorrhizae, chitosan, or EDTA-Zinc on the growth, oil content, and chemical analysis of Cynara cardunculus plants”

✓ located verbatim in the source · abstract, characters 151–363 · show in text ↑
e6

“The highest amount of unsaturated fatty acids was found in azolla liquid and mycorrhizae treatments”

✓ located verbatim in the source · abstract, characters 1186–1285 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Mycorrhizae gave the highest amount of unsaturated fatty acids in cardoon oil.

Taxon
Cynara cardunculusCynara cardunculuse0
Treatment
mycorrhizaee6
Outcome
amount of unsaturated fatty acidse6
Direction
higheste6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cynara cardunculus
Cynara cardunculus · TaxonBurkhard Mücke · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“two-season field experiments were conducted in clay loam soil to evaluate the efficacy of azolla, mycorrhizae, chitosan, or EDTA-Zinc on the growth, oil content, and chemical analysis of Cynara cardunculus plants”

✓ located verbatim in the source · abstract, characters 151–363 · show in text ↑
e6

“The highest amount of unsaturated fatty acids was found in azolla liquid and mycorrhizae treatments”

✓ located verbatim in the source · abstract, characters 1186–1285 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors recommend applying azolla, mycorrhizae and chitosan alone or with EDTA-Zinc to enhance growth, oil percentage and oil yield of cardoon.

Taxon
Cynara cardunculusCynara cardunculuse0
Treatment
application of azolla, mycorrhizae, and chitosane7
Outcome
growth, oil percentage, and oil yielde7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cynara cardunculus
Cynara cardunculus · TaxonBurkhard Mücke · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“two-season field experiments were conducted in clay loam soil to evaluate the efficacy of azolla, mycorrhizae, chitosan, or EDTA-Zinc on the growth, oil content, and chemical analysis of Cynara cardunculus plants”

✓ located verbatim in the source · abstract, characters 151–363 · show in text ↑
e7

“Our findings indicated that the application of azolla, mycorrhizae, and chitosan can be used alone or as a complement to EDTA-Zinc to enhance growth, oil percentage, and oil yield in cardoon plants”

✓ located verbatim in the source · abstract, characters 1287–1484 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Applying the biofertilizers had a significant effect by accelerating cardoon plant growth.

Taxon
Cynara cardunculusCynara cardunculuse0
Treatment
Applying these biofertilizerse1
Outcome
plant growthe1
Direction
acceleratinge1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cynara cardunculus
Cynara cardunculus · TaxonBurkhard Mücke · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“two-season field experiments were conducted in clay loam soil to evaluate the efficacy of azolla, mycorrhizae, chitosan, or EDTA-Zinc on the growth, oil content, and chemical analysis of Cynara cardunculus plants”

✓ located verbatim in the source · abstract, characters 151–363 · show in text ↑
e1

“Applying these biofertilizers had a significant effect by accelerating plant growth”

✓ located verbatim in the source · abstract, characters 1486–1569 · show in text ↑

chitosan · physiology: pigments · Cynara cardunculus (cardoon)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

chitosan · yield · Cynara cardunculus (cardoon)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

inoculation · growth · Cynara cardunculus (cardoon)Positive and negative results

7 papers: 6 positive, 1 negative or no effect

5 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

3 papers more in this group

See all 7 papers →

fertilization · growth · Cynara cardunculus (cardoon)Positive and negative results

9 papers: 8 positive, 1 negative or no effect

7 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. positive

5 papers more in this group

See all 9 papers →

fertilization · yield · Cynara cardunculus (cardoon)Positive and negative results

14 papers: 13 positive, 1 negative or no effect

12 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. positive

10 papers more in this group

See all 14 papers →

application · physiology: pigments · Cynara cardunculus (cardoon)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

mycorrhiza · quality: phytochemicals · Cynara cardunculus (cardoon)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

botanical · yield · Cynara cardunculus (cardoon)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

8 of 10 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.