Abstract
Roots play a critical role in acquisition and utilization of nitrogen in wheat, influencing nitrogen use efficiency (NUE), and ultimately determining yield. However, the detailed responses of root tips to fluctuations in nitrogen availability and the underlying regulatory mechanisms enabling adaptation to nitrogen-limited conditions, remain elusive. In this study, we used single-cell nuclear transcriptomics of the high-nitrogen utilization variety (HNV) Zhengmai 1860 (ZM1860) to construct a comprehensive map of root tip cells under both controlled and nitrogen starvation (N-starv) conditions. Identification of various cell types and their associated genes highlighted the diversity of cellular processes. Using single-nucleus consensus weighted gene co-expression network analysis (hdWGCNA), we identified key modules central to nitrogen metabolism. These identified the prominent role of epidermal cells (EC). The gene TaGS1.2 , which is involved in glutamine synthesis, exhibited increased expression under nitrogen-deficient conditions, validating its functional significance in nutrient acquisition. Serving as a key functional gene that adapts to nitrogen-deficient conditions this gene also positively regulated root development. Analysis of the transcriptional regulatory network in EC further revealed the pivotal role of TaGS1.2 in the nitrogen metabolism network. We also uncovered mechanisms that enhance cell-to-cell communication in nitrogen-deficient environments by identifying specific receptors. Single-cell nuclear transcriptome mapping offers valuable insights into the complex responses of root tip cells to nitrogen scarcity and guides future breeding strategies aimed at developing more nitrogen-efficient wheat varieties.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
The study generated a root-tip single-cell map for Zhengmai 1860 under control and nitrogen starvation conditions.
- Taxon
- wheat
Triticum aestivumt0 - Material
- Zhengmai 1860e1
- Organ / stage
- root tip cellse0
- Outcome
- comprehensive map of root tip cellse0
- Comparator
- controllede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“construct a comprehensive map of root tip cells under both controlled and nitrogen starvation (N-starv) conditions”
e1“Zhengmai 1860 (ZM1860)”
t0“wheat”
Claim · model-written summary
The single-cell analysis identified diverse root-tip cell types and associated genes.
- Taxon
- wheatTriticum aestivumt0
- Organ / stage
- cell typese0
- Outcome
- various cell types and their associated genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Identification of various cell types and their associated genes highlighted the diversity of cellular processes”
t0“wheat”
Claim · model-written summary
The analysis identified key modules central to nitrogen metabolism.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- key modules central to nitrogen metabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified key modules central to nitrogen metabolism”
t0“wheat”
Claim · model-written summary
Epidermal cells had a prominent role in the identified nitrogen metabolism network.
- Taxon
- wheatTriticum aestivumt0
- Organ / stage
- epidermal cellse0
- Outcome
- prominent role of epidermal cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These identified the prominent role of epidermal cells (EC)”
t0“wheat”
Claim · model-written summary
TaGS1.2 expression increased under nitrogen-deficient conditions.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- nitrogen-deficient conditionse0
- Gene
- TaGS1.2e0
- Outcome
- expressione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaGS1.2 , which is involved in glutamine synthesis, exhibited increased expression under nitrogen-deficient conditions”
t0“wheat”
Claim · model-written summary
TaGS1.2 positively regulated root development.
- Taxon
- wheatTriticum aestivumt0
- Organ / stage
- roote0
- Gene
- TaGS1.2e1
- Outcome
- root developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this gene also positively regulated root development”
e1“The gene TaGS1.2”
t0“wheat”
Claim · model-written summary
The epidermal-cell transcriptional network revealed a pivotal role for TaGS1.2 in nitrogen metabolism.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaGS1.2e0
- Outcome
- pivotal role of TaGS1.2 in the nitrogen metabolism networke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcriptional regulatory network in EC further revealed the pivotal role of TaGS1.2 in the nitrogen metabolism network”
t0“wheat”
Claim · model-written summary
Specific receptors were identified as mediators of enhanced cell-to-cell communication in nitrogen deficiency.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- nitrogen-deficient environmentse0
- Outcome
- cell-to-cell communicatione0
- Qualifier
- specific receptorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mechanisms that enhance cell-to-cell communication in nitrogen-deficient environments by identifying specific receptors”
t0“wheat”
The same questions in other papers
nitrogen · molecular: expression · Triticum aestivum (wheat)Positive and negative results
10 papers in the same direction as this paper · 26 in the opposite direction
This paper is about lacking the nutrient, so it counts the other way round here.
- positive
- positive
- positive
33 papers more in this group
See all 37 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.