Abstract
Olive (Olea europaea L.). cv. Koroneiki belongs to the family Oleaceae, which is an evergreen plant of Mediterranean origin. Commonly, this plant is the source of olive oil used for various purposes in human life. To determine the biochemical characteristics of the extracted olive oil from specific callus lines of common olive, we investigated the effects of several hormones on callus induction from field-grown plants. Mature leaves, nodal, and internodal explants were surface-sterilized and inoculated on MS medium. Plant growth regulators in varying amounts were added to MS medium (2,4-D, BAP, IBA, NAA, or TDZ) alone or in combinations, forming a total of 29 treatments for callus induction. The highest (95.45%) callus induction was obtained with 8 + 2 µM BAP + IBA after 35 days of initial culture from leaf explants, followed by 6 µM 2,4-D (75.55%) under the dark culture room conditions. Internode was unable to form callus. Off white, yellowish, fluffy, friable, proliferating calluses were obtained from leaf explants. Such highly proliferating calluses were selected as callus cell lines (CL); CL1 (2, 4-D 4 µM) and CL2 (2, 4-D 6 µM) for oil extraction and subsequent biochemical characteristics of acid value and saponification number. Results demonstrated that CL1 showed the highest acid value (1.25) and saponification number (183). This was followed by CL2, which showed acid values of 1.42 and 1.84, and saponification values of 1.84 and 1.42, respectively. The present study demonstrated an efficient callus induction protocol for subsequent determination of biochemical attributes from the extracted oil of in vitro dedifferentiated olive tissues.
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Claims 8
Claim · model-written summary
BAP plus IBA gave the highest leaf explant callus induction of 95.45%.
- Taxon
- Olive
Olea europaeat0 - Organ / stage
- leaf explantse0
- Treatment
- 8 + 2 µM BAP + IBAe0
- Outcome
- callus inductione0
- Direction
- higheste0
- Value
- 95.45%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest (95.45%) callus induction was obtained with 8 + 2 µM BAP + IBA after 35 days of initial culture from leaf explants”
t0“Olive”
Claim · model-written summary
6 µM 2,4-D induced callus at 75.55% under dark conditions.
- Taxon
- OliveOlea europaeat0
- Treatment
- 6 µM 2,4-De0
- Outcome
- callus inductione1
- Value
- 75.55%e0
- Setting
- dark culture room conditionse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“followed by 6 µM 2,4-D (75.55%) under the dark culture room conditions”
t0“Olive”
e1“callus induction was obtained with 8 + 2 µM BAP + IBA”
Claim · model-written summary
Internode explants did not form callus.
- Taxon
- OliveOlea europaeat0
- Organ / stage
- Internodee0
- Outcome
- unable to form calluse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Internode was unable to form callus.”
t0“Olive”
Claim · model-written summary
Leaf explants produced off-white, yellowish, fluffy, friable, proliferating callus.
- Taxon
- OliveOlea europaeat0
- Organ / stage
- leaf explantse0
- Outcome
- Off white, yellowish, fluffy, friable, proliferating callusese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Off white, yellowish, fluffy, friable, proliferating calluses were obtained from leaf explants.”
t0“Olive”
Claim · model-written summary
CL1 had an acid value of 1.25, reported as highest.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CL1 showed the highest acid value (1.25)”
t0“Olive”
Claim · model-written summary
CL1 had a saponification number of 183.
- Taxon
- OliveOlea europaeat0
- Material
- CL1e0
- Outcome
- saponification numbere0
- Value
- 183e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CL1 showed the highest acid value (1.25) and saponification number (183)”
t0“Olive”
Claim · model-written summary
CL2 was reported with acid values of 1.42 and 1.84.
- Taxon
- OliveOlea europaeat0
- Material
- CL2e0
- Outcome
- acid valuese0
- Value
- 1.42 and 1.84e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CL2, which showed acid values of 1.42 and 1.84”
t0“Olive”
Claim · model-written summary
CL2 was reported with saponification values of 1.84 and 1.42.
- Taxon
- OliveOlea europaeat0
- Material
- CL2e1
- Outcome
- saponification valuese0
- Value
- 1.84 and 1.42e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“saponification values of 1.84 and 1.42”
e1“CL2, which showed acid values”
t0“Olive”
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