Abstract
Olive (Olea europaea L.) is an economically important crop because of its fruit and oil. Successful olive micropropagation is highly dependence on cultivar, shoot proliferation rate, which is generally low, the rooting of micropropagated olive plantlets is difficult, and the rate of post-transplanting losses is high. In addition, hyperhydricity, a common problem in vitro culture was found to be prevalent. The aim of this study was to establish a micropropagation system for the Turkish O. europaea L. cv. Gemlik. Initially, five different basal media were tested to determine appropriate medium for establishment of in vitro culture and Woody Plant Medium (WPM) was the most efficient. Nodal explants were cultured on WPM containing different plant growth regulators (PGRs) for shoot regeneration. Maximum regeneration frequency and number of shoots per explant were achieved from nodal explants cultured on WPM supplemented with 4.0 mg/L 6-benzyladenine (BA). However, all cultures showed high hyperhydricity and an experimentation was also conducted to resolve the hyperhydricity problem. Hyperhydricity was prevented by changing the gelling agent to Agar-Agar. The shoots regenerated from nodal explants and still attached to initial woody nodal explant were transferred to four different medium formulations each containing 2.0 mg/L zeatin (ZEA) for shoot elongation. Modified Olive Medium (MOM2: OM with three times the concentrations of KNO3) fortified with 2.0 mg/L ZEA was found to be the best for shoot elongation. The elongated shoots were rooted on Olive medium (OM) containing 160 mg/L Putrescine, 1.5 mg/L naphthaleneacetic acid (NAA), 30 g/L mannitol and solidified with 0.65% (w/v) Agar-Agar. Finally, all plantlets were successfully acclimatized in a climate chamber and the plants were transferred to greenhouse conditions.
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Claims 7
Claim · model-written summary
Woody Plant Medium was the most efficient basal medium for establishing olive culture.
- Taxon
- olive (Olea europaea L.)
Olea europaeat0 - Material
- Gemlikt0
- Treatment
- Woody Plant Medium (WPM)e0
- Outcome
- most efficiente0
- Direction
- most efficiente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Woody Plant Medium (WPM) was the most efficient”
t0“olive (Olea europaea L.) cultivar ‘Gemlik’”
Claim · model-written summary
WPM with 4.0 mg/L BA gave maximum regeneration frequency and shoots per explant.
- Taxon
- olive (Olea europaea L.)Olea europaeat0
- Material
- Gemlikt0
- Organ / stage
- nodal explantse0
- Treatment
- WPM supplemented with 4.0 mg/L 6-benzyladenine (BA)e0
- Outcome
- regeneration frequency and number of shoots per explante0
- Direction
- Maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum regeneration frequency and number of shoots per explant were achieved from nodal explants cultured on WPM supplemented with 4.0 mg/L 6-benzyladenine (BA)”
t0“olive (Olea europaea L.) cultivar ‘Gemlik’”
Claim · model-written summary
All cultures had high hyperhydricity.
- Taxon
- olive (Olea europaea L.)Olea europaeat0
- Material
- Gemlikt0
- Outcome
- high hyperhydricitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all cultures showed high hyperhydricity”
t0“olive (Olea europaea L.) cultivar ‘Gemlik’”
Claim · model-written summary
Changing the gelling agent to Agar-Agar prevented hyperhydricity.
- Taxon
- olive (Olea europaea L.)Olea europaeat0
- Material
- Gemlikt0
- Treatment
- changing the gelling agent to Agar-Agare0
- Outcome
- Hyperhydricitye0
- Direction
- preventede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hyperhydricity was prevented by changing the gelling agent to Agar-Agar”
t0“olive (Olea europaea L.) cultivar ‘Gemlik’”
Claim · model-written summary
MOM2 with 2.0 mg/L ZEA was best for shoot elongation.
- Taxon
- olive (Olea europaea L.)Olea europaeat0
- Material
- Gemlikt0
- Treatment
- Modified Olive Medium (MOM2: OM with three times the concentrations of KNO3) fortified with 2.0 mg/L ZEAe0
- Outcome
- shoot elongatione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Modified Olive Medium (MOM2: OM with three times the concentrations of KNO3) fortified with 2.0 mg/L ZEA was found to be the best for shoot elongation”
t0“olive (Olea europaea L.) cultivar ‘Gemlik’”
Claim · model-written summary
Elongated shoots rooted on supplemented Olive medium.
- Taxon
- olive (Olea europaea L.)Olea europaeat0
- Material
- Gemlikt0
- Organ / stage
- elongated shootse0
- Treatment
- Olive medium (OM) containing 160 mg/L Putrescine, 1.5 mg/L naphthaleneacetic acid (NAA), 30 g/L mannitol and solidified with 0.65% (w/v) Agar-Agare0
- Outcome
- rootede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The elongated shoots were rooted on Olive medium (OM) containing 160 mg/L Putrescine, 1.5 mg/L naphthaleneacetic acid (NAA), 30 g/L mannitol and solidified with 0.65% (w/v) Agar-Agar”
t0“olive (Olea europaea L.) cultivar ‘Gemlik’”
Claim · model-written summary
All plantlets acclimatized in a climate chamber and were transferred to a greenhouse.
- Taxon
- olive (Olea europaea L.)Olea europaeat0
- Material
- Gemlikt0
- Outcome
- successfully acclimatizede0
- Setting
- a climate chambere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all plantlets were successfully acclimatized in a climate chamber and the plants were transferred to greenhouse conditions”
t0“olive (Olea europaea L.) cultivar ‘Gemlik’”
The same questions in other papers
zeatin · growth · Olea europaea (common olive)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
N-benzyladenine · growth · Olea europaea (common olive)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
1 paper more in this group
See all 5 papers →in vitro · growth · Olea europaea (common olive)Positive and negative results
16 papers in the same direction as this paper · 2 in the opposite direction
- positive
- negative
- negative
15 papers more in this group
See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.