Abstract
Abstract Sugar content serves as a crucial determinant of fruit flavor quality and nutritional value. Calcium plays extensive regulatory roles in fruit development and quality formation, yet the molecular mechanisms underlying calcium-mediated sugar accumulation remain poorly understood. In this study, we demonstrate that calcium treatment enhances sugar accumulation in both citrus fruits and calli, concomitant with upregulated expression of the sucrose transporter gene CsSWEET17. Functional characterization revealed that the membrane-localized CsSWEET17 protein exhibits sucrose transport activity. Transgenic overexpression of CsSWEET17 in citrus juice sacs, calli and heterologous tomato systems consistently elevated sucrose levels. Conversely, suppression of CsSWEET17 expression through either virus-induced gene silencing or RNA interference significantly reduced sucrose content in citrus. Further investigation identified CsMYB36 as a calcium-responsive transcription factor that directly activates CsSWEET17 expression. Transgenic validation demonstrated that both calcium signaling and CsMYB36-mediated sucrose accumulation strictly depend on CsSWEET17 transcriptional regulation. Our findings elucidate a novel calcium-MYB36-SWEET17 regulatory module controlling sucrose accumulation, providing molecular insights into calcium-based strategies in citrus quality improvement and informing fundamental mechanisms of sugar transporter regulation in fruit crops.
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Claims 8
Claim · model-written summary
Calcium treatment enhanced sugar accumulation in citrus fruits and calli.
- Organ / stage
- fruits and callie0
- Treatment
- calcium treatmente0
- Outcome
- sugar accumulatione0
- Direction
- enhancese0
CitrusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus”
e0“calcium treatment enhances sugar accumulation in both citrus fruits and calli”
Claim · model-written summary
Calcium treatment was accompanied by upregulated expression of the sucrose transporter gene CsSWEET17 in citrus.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus”
e0“calcium treatment”
e1“upregulated expression of the sucrose transporter gene CsSWEET17”
Claim · model-written summary
The membrane-localized CsSWEET17 protein has sucrose transport activity.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus”
e0“the membrane-localized CsSWEET17 protein exhibits sucrose transport activity”
Claim · model-written summary
Transgenic overexpression of CsSWEET17 in citrus juice sacs and calli elevated sucrose levels.
- Organ / stage
- juice sacs, callie0
- Treatment
- Transgenic overexpression of CsSWEET17e0
- Gene
- CsSWEET17e0
- Outcome
- sucrose levelse0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus”
e0“Transgenic overexpression of CsSWEET17 in citrus juice sacs, calli and heterologous tomato systems consistently elevated sucrose levels”
Claim · model-written summary
Transgenic overexpression of CsSWEET17 in a heterologous tomato system elevated sucrose levels.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- Transgenic overexpression of CsSWEET17e0
- Gene
- CsSWEET17e0
- Outcome
- sucrose levelse0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic overexpression of CsSWEET17 in citrus juice sacs, calli and heterologous tomato systems consistently elevated sucrose levels”
Claim · model-written summary
Suppression of CsSWEET17 by virus-induced gene silencing or RNA interference significantly reduced sucrose content in citrus.
- Treatment
- suppression of CsSWEET17 expression through either virus-induced gene silencing or RNA interferencee0
- Gene
- CsSWEET17e0
- Outcome
- sucrose contente0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus”
e0“suppression of CsSWEET17 expression through either virus-induced gene silencing or RNA interference significantly reduced sucrose content in citrus”
Claim · model-written summary
CsMYB36 is a calcium-responsive transcription factor that directly activates CsSWEET17 expression in citrus.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus”
e0“CsMYB36 as a calcium-responsive transcription factor that directly activates CsSWEET17 expression”
Claim · model-written summary
Calcium signaling and CsMYB36-mediated sucrose accumulation depend on CsSWEET17 transcriptional regulation in citrus.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus”
e0“both calcium signaling and CsMYB36-mediated sucrose accumulation strictly depend on CsSWEET17 transcriptional regulation”
The same questions in other papers
calcium atom · molecular: metabolites · CitrusPositive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
calcium atom · molecular: expression · CitrusResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.