Paper · 2025 · Entomological News

Effectiveness of the Usher Plant Extracts from High Altitude as a Safe Control Method Against Pomegranate Aphid, Aphis punicae, on Pomegranate Trees

Samy M. Sayed; Laila A. Al-Shuraym; Lamya A. Alkeridis; et al.

6
claims
15
verbatim quotes
0
citations
4
research areas
—
Not open access

Abstract

The pomegranate (Punica granatum L.) (Lythraceae) is commonly infested with many insect pests, especially aphids. The pomegranate aphid, Aphis punicae (Hemiptera: Aphididae) is the important insect pest on pomegranate trees where it infests leaves, buds and fruits. It causes damage that reduces the yield of pomegranate fruits. This investigation estimated the control efficacy of leaf and fruit extracts of the usher plant, Calotropis procera (Apocynaceae) on A. punicae. The phenols and flavonoids in both extracts were determined by HPLC analysis. Total phenols and flavonoids were 23.089 and 2.223 mg/g in leaves and fruits, respectively. We found that LC50 and LC90 values of leaf extract (251.3 and 1017.9 µg/mL, respectively) were significantly lower than those of fruit extract (622.5 and 2521.8 µg/mL, respectively). Field experiments using leaf extract resulted in an LC50 within one- and two-week intervals. Results of field application showed there was always significantly greater aphid reductions in the treatment where the spray was two weeks old over treatment that was one week old. However, there were no differences always noticed for spraying that was a week old in both treatments. This result stated that the application of C. procera leaf extract at one-week intervals is efficient to control pomegranate aphids with more than 90% of a reduction rate. Further experiments could be done to estimate the safety of this plant extract on beneficial organisms such as insect predators and parasitoids associated with the insect pests.

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Claims 6

Observationobservedoriginal resultmeasured

Claim · model-written summary

Total phenols in leaves were reported as 23.089 mg/g.

Taxon
Calotropis proceraCalotropis procerae1
Organ / stage
leavese0
Outcome
Total phenolse0
Value
23.089e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Calotropis procera
Calotropis procera · TaxonWilfredo Rodríguez · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Total phenols and flavonoids were 23.089 and 2.223 mg/g in leaves and fruits, respectively.”

✓ located verbatim in the source · abstract, characters 552–643 · show in text ↑
e1

“usher plant, Calotropis procera”

✓ located verbatim in the source · abstract, characters 413–444 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Total flavonoids in fruits were reported as 2.223 mg/g.

Taxon
Calotropis proceraCalotropis procerae1
Organ / stage
fruitse0
Outcome
flavonoidse0
Value
2.223 mg/ge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Calotropis procera
Calotropis procera · TaxonWilfredo Rodríguez · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Total phenols and flavonoids were 23.089 and 2.223 mg/g in leaves and fruits, respectively.”

✓ located verbatim in the source · abstract, characters 552–643 · show in text ↑
e1

“usher plant, Calotropis procera”

✓ located verbatim in the source · abstract, characters 413–444 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Leaf extract had a significantly lower LC50 against pomegranate aphid than fruit extract.

Taxon
PomegranatePunica granatumt0
Treatment
leaf extracte0
Interactor
Aphis punicaeAphis punicaee1pest
Outcome
LC50e0
Direction
lowere0
Value
251.3e0
Qualifier
622.5e0
Comparator
fruit extracte0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗
Aphis punicae
Aphis punicae · InteractorW Rao · CC BY 4.0 · iNaturalist ↗

Verbatim

t0

“Pomegranate Trees”

✓ located verbatim in the source · title, characters 131–148 · show in text ↑
e0

“We found that LC50 and LC90 values of leaf extract (251.3 and 1017.9 µg/mL, respectively) were significantly lower than those of fruit extract (622.5 and 2521.8 µg/mL, respectively).”

✓ located verbatim in the source · abstract, characters 644–826 · show in text ↑
e1

“pomegranate aphid, Aphis punicae”

✓ located verbatim in the source · abstract, characters 118–150 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Leaf extract had a significantly lower LC90 against pomegranate aphid than fruit extract.

Taxon
PomegranatePunica granatumt0
Treatment
leaf extracte0
Interactor
Aphis punicaeAphis punicaee1pest
Outcome
LC90e0
Direction
lowere0
Value
1017.9 µg/mLe0
Qualifier
2521.8 µg/mLe0
Comparator
fruit extracte0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗
Aphis punicae
Aphis punicae · InteractorW Rao · CC BY 4.0 · iNaturalist ↗

Verbatim

t0

“Pomegranate Trees”

✓ located verbatim in the source · title, characters 131–148 · show in text ↑
e0

“We found that LC50 and LC90 values of leaf extract (251.3 and 1017.9 µg/mL, respectively) were significantly lower than those of fruit extract (622.5 and 2521.8 µg/mL, respectively).”

✓ located verbatim in the source · abstract, characters 644–826 · show in text ↑
e1

“pomegranate aphid, Aphis punicae”

✓ located verbatim in the source · abstract, characters 118–150 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

A two-week-old field spray reduced aphids significantly more than a one-week-old spray.

Taxon
PomegranatePunica granatumt0
Treatment
spray was two weeks olde0
Interactor
Aphis punicaeAphis punicaee1pest
Outcome
aphid reductionse0
Direction
greatere0
Comparator
treatment that was one week olde0
Setting
field applicatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗
Aphis punicae
Aphis punicae · InteractorW Rao · CC BY 4.0 · iNaturalist ↗

Verbatim

t0

“Pomegranate Trees”

✓ located verbatim in the source · title, characters 131–148 · show in text ↑
e0

“Results of field application showed there was always significantly greater aphid reductions in the treatment where the spray was two weeks old over treatment that was one week old.”

✓ located verbatim in the source · abstract, characters 920–1100 · show in text ↑
e1

“pomegranate aphid, Aphis punicae”

✓ located verbatim in the source · abstract, characters 118–150 · show in text ↑
Intervention effectauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

Weekly Calotropis leaf extract application reduced pomegranate aphids by more than 90%.

Taxon
PomegranatePunica granatumt0
Treatment
C. procera leaf extract at one-week intervalse0
Interactor
pomegranate aphidse0pest
Outcome
pomegranate aphidse0
Direction
reductione0
Value
more than 90%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Pomegranate Trees”

✓ located verbatim in the source · title, characters 131–148 · show in text ↑
e0

“the application of C. procera leaf extract at one-week intervals is efficient to control pomegranate aphids with more than 90% of a reduction rate.”

✓ located verbatim in the source · abstract, characters 1228–1375 · show in text ↑

application · Aphis punicae · Punica granatum (pomegranate)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

botanical · Aphis punicae · Punica granatum (pomegranate)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

botanical · pest · Punica granatum (pomegranate)Results agree

5 papers: 5 positive, 0 negative or no effect

4 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.