Paper · 2025 · Journal of Applied Research in Plant Sciences

Iron Biofortification of Wheat Grains with Various Iron Fertilization Techniques

Saima Kalsoom Babar; Zeeshan Mashori; Nizamuddin Depar; et al.

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citations
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Open access · cc-by

Abstract

Most Pakistani soils are deficient in available iron (Fe), which limits crop productivity. Biofortification offers a sustainable solution by enhancing nutrient content in edible parts. This field experiment, conducted at the Nuclear Institute of Agriculture (NIA), Tandojam during the 2023–24 Rabi season, evaluated the effectiveness of iron application methods on wheat (variety NBW-232). The study used a randomized complete block design (RCBD) with four treatments and three replications on silty loam soil. Treatments included: T1 (control), T2 (12 kg Fe ha⁻¹ via broadcasting), T3 (12 kg Fe ha⁻¹ as Fe-coated urea), and T4 (seed priming with 2% FeSO₄·7H₂O). A uniform NPK dose (150-80-50 kg ha⁻¹) was applied. Results showed that Fe application significantly improved growth, yield, and Fe accumulation, with Fe-coated urea (T3) being the most effective. Iron coated urea (T3) succeeded to obtain the highest number of grains per spike (60.2), grain yield (5533.3 kg ha⁻¹), nitrogen (2.9%), and Fe content (119.3 mg kg⁻¹), while reducing phytic acid (1.807 mg g⁻¹) concentration (p<0.01). In conclusion, Fe-coated urea markedly enhanced wheat productivity and its nutritional value

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 8

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Fe application significantly improved wheat growth.

Treatment
Fe applicatione0
Outcome
growthe0
Direction
improvede0
Comparator
controle2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Fe application significantly improved growth, yield, and Fe accumulation”

✓ located verbatim in the source · abstract, characters 735–807 · show in text ↑
e1

“wheat (variety NBW-232)”

✓ located verbatim in the source · abstract, characters 365–388 · show in text ↑
e2

“T1 (control)”

✓ located verbatim in the source · abstract, characters 532–544 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Fe application significantly improved yield.

Treatment
Fe applicatione0
Outcome
yielde0
Direction
improvede0
Comparator
controle2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Fe application significantly improved growth, yield, and Fe accumulation”

✓ located verbatim in the source · abstract, characters 735–807 · show in text ↑
e1

“wheat (variety NBW-232)”

✓ located verbatim in the source · abstract, characters 365–388 · show in text ↑
e2

“T1 (control)”

✓ located verbatim in the source · abstract, characters 532–544 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Fe application significantly improved Fe accumulation.

Treatment
Fe applicatione0
Outcome
Fe accumulatione0
Direction
improvede0
Comparator
controle2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Fe application significantly improved growth, yield, and Fe accumulation”

✓ located verbatim in the source · abstract, characters 735–807 · show in text ↑
e1

“wheat (variety NBW-232)”

✓ located verbatim in the source · abstract, characters 365–388 · show in text ↑
e2

“T1 (control)”

✓ located verbatim in the source · abstract, characters 532–544 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Fe-coated urea gave the highest grains per spike, 60.2.

Treatment
Iron coated urea (T3)e0
Outcome
number of grains per spikee0
Direction
higheste0
Value
60.2e0
Comparator
controle2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Iron coated urea (T3) succeeded to obtain the highest number of grains per spike (60.2)”

✓ located verbatim in the source · abstract, characters 860–947 · show in text ↑
e1

“wheat (variety NBW-232)”

✓ located verbatim in the source · abstract, characters 365–388 · show in text ↑
e2

“T1 (control)”

✓ located verbatim in the source · abstract, characters 532–544 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Fe-coated urea gave the highest grain yield, 5533.3 kg ha⁻¹.

Treatment
Iron coated urea (T3)e0
Outcome
grain yielde0
Direction
higheste0
Value
5533.3 kg ha⁻¹e0
Comparator
controle2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Iron coated urea (T3) succeeded to obtain the highest number of grains per spike (60.2), grain yield (5533.3 kg ha⁻¹)”

✓ located verbatim in the source · abstract, characters 860–977 · show in text ↑
e1

“wheat (variety NBW-232)”

✓ located verbatim in the source · abstract, characters 365–388 · show in text ↑
e2

“T1 (control)”

✓ located verbatim in the source · abstract, characters 532–544 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Fe-coated urea gave the highest nitrogen content, 2.9%.

Treatment
Iron coated urea (T3)e0
Outcome
nitrogene0
Direction
higheste0
Value
2.9%e0
Comparator
controle2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Iron coated urea (T3) succeeded to obtain the highest number of grains per spike (60.2), grain yield (5533.3 kg ha⁻¹), nitrogen (2.9%)”

✓ located verbatim in the source · abstract, characters 860–994 · show in text ↑
e1

“wheat (variety NBW-232)”

✓ located verbatim in the source · abstract, characters 365–388 · show in text ↑
e2

“T1 (control)”

✓ located verbatim in the source · abstract, characters 532–544 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Fe-coated urea gave the highest Fe content, 119.3 mg kg⁻¹.

Treatment
Iron coated urea (T3)e0
Outcome
Fe contente0
Direction
higheste0
Value
119.3 mg kg⁻¹e0
Comparator
controle2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Iron coated urea (T3) succeeded to obtain the highest number of grains per spike (60.2), grain yield (5533.3 kg ha⁻¹), nitrogen (2.9%), and Fe content (119.3 mg kg⁻¹)”

✓ located verbatim in the source · abstract, characters 860–1026 · show in text ↑
e1

“wheat (variety NBW-232)”

✓ located verbatim in the source · abstract, characters 365–388 · show in text ↑
e2

“T1 (control)”

✓ located verbatim in the source · abstract, characters 532–544 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Fe-coated urea reduced phytic acid concentration to 1.807 mg g⁻¹.

Treatment
Iron coated urea (T3)e0
Outcome
phytic acid (1.807 mg g⁻¹) concentratione0
Direction
reducinge0
Value
1.807 mg g⁻¹e0
Comparator
controle2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Iron coated urea (T3) succeeded to obtain the highest number of grains per spike (60.2), grain yield (5533.3 kg ha⁻¹), nitrogen (2.9%), and Fe content (119.3 mg kg⁻¹), while reducing phytic acid (1.807 mg g⁻¹) concentration (p<0.01).”

✓ located verbatim in the source · abstract, characters 860–1093 · show in text ↑
e1

“wheat (variety NBW-232)”

✓ located verbatim in the source · abstract, characters 365–388 · show in text ↑
e2

“T1 (control)”

✓ located verbatim in the source · abstract, characters 532–544 · show in text ↑

iron atom · growth · Triticum aestivum (wheat)Results differ

54 papers: 41 positive, 13 negative or no effect

40 papers in the same direction as this paper · 12 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. negative
  3. negative

50 papers more in this group

See all 54 papers →

iron atom · nutrient: content · Triticum aestivum (wheat)Results differ

74 papers: 61 positive, 13 negative or no effect

60 papers in the same direction as this paper · 12 in the opposite direction · 1 reporting no difference from an alternative

  1. positive
  2. no effect
  3. no effect

70 papers more in this group

See all 74 papers →

iron atom · yield · Triticum aestivum (wheat)Results differ

99 papers: 92 positive, 7 negative or no effect

91 papers in the same direction as this paper · 7 in the opposite direction

  1. positive
  2. no effect
  3. no effect

95 papers more in this group

See all 99 papers →

iron atom · molecular: metabolites · Triticum aestivum (wheat)Positive and negative results

17 papers: 11 positive, 6 negative or no effect

10 papers in the same direction as this paper · 6 in the opposite direction

  1. no effect
  2. negative
  3. negative

13 papers more in this group

See all 17 papers →

nitrogen · molecular: metabolites · Triticum aestivum (wheat)Positive and negative results

166 papers: 114 positive, 52 negative or no effect

113 papers in the same direction as this paper · 49 in the opposite direction · 3 reporting no difference from an alternative

  1. negative
  2. no effect
  3. no effect

162 papers more in this group

See all 166 papers →

nitrogen · nutrient: content · Triticum aestivum (wheat)Positive and negative results

479 papers: 410 positive, 69 negative or no effect

409 papers in the same direction as this paper · 64 in the opposite direction · 5 reporting no difference from an alternative

  1. no effect
  2. negative
  3. no effect

475 papers more in this group

See all 479 papers →

nitrogen · yield · Triticum aestivum (wheat)Positive and negative results

2,061 papers: 1,737 positive, 324 negative or no effect

1,736 papers in the same direction as this paper · 285 in the opposite direction · 39 reporting no difference from an alternative

  1. negative
  2. negative
  3. negative

2,057 papers more in this group

See all 2,061 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.