Abstract
Calcium signaling is essential for coordinating plant responses to diverse stimuli and regulating growth and development. Among calcium sensors, calmodulin (CaM) and CaM-like proteins (CMLs) represent a class that, despite increasing research, remains incompletely characterized in wheat, with many interacting partners and biological functions remaining largely elusive. This study conducted bioinformatics analyses of subgroup II CaM/CMLs, characterizing their phylogenetic relationships, conserved motifs, sequence features, and cis-elements. Expression analysis revealed that TaCML49-B was significantly upregulated in roots under salt stress. Moreover, TaCML49-B was localized to nucleus, cytoplasm, and membrane. Function characterization demonstrated that overexpression of TaCML49-B in Arabidopsis enhanced salt tolerance, whereas the BSMV-VIGS silencing of TaCML49-B reduced salt resistance in wheat. Furthermore, STRING database prediction analysis and bimolecular fluorescence complementation (BiFC) assay confirmed that TaCML49-B can physically interact with TaIQD23, which encodes an IQ67 domain protein, suggesting its potential involvement in the salt stress signaling pathway. Collectively, our findings indicate that TaCML49-B functions as a positive role in wheat salt stress response, thereby providing novel insights into the functions of TaCML genes and calcium signaling in wheat.
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Claims 5
Claim · model-written summary
TaCML49-B was significantly upregulated in roots under salt stress.
- Taxon
- wheat
Triticum aestivumt0 - Organ / stage
- rootse0
- Treatment
- salt stresse0
- Gene
- TaCML49-Be0
- Outcome
- TaCML49-Be0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression analysis revealed that TaCML49-B was significantly upregulated in roots under salt stress.”
t0“Wheat”
Claim · model-written summary
TaCML49-B localized to nucleus, cytoplasm and membrane.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaCML49-Be0
- Outcome
- localized to nucleus, cytoplasm, and membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaCML49-B was localized to nucleus, cytoplasm, and membrane.”
t0“Wheat”
Claim · model-written summary
Overexpression of TaCML49-B enhanced salt tolerance in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- overexpression of TaCML49-Be0
- Gene
- TaCML49-Be0
- Outcome
- salt tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of TaCML49-B in Arabidopsis enhanced salt tolerance”
Claim · model-written summary
Silencing TaCML49-B reduced salt resistance in wheat.
- Taxon
- wheatTriticum aestivume0
- Treatment
- BSMV-VIGS silencing of TaCML49-Be0
- Gene
- TaCML49-Be0
- Outcome
- salt resistancee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the BSMV-VIGS silencing of TaCML49-B reduced salt resistance in wheat.”
Claim · model-written summary
TaCML49-B physically interacted with TaIQD23.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaCML49-Be0
- Outcome
- physically interact with TaIQD23e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“bimolecular fluorescence complementation (BiFC) assay confirmed that TaCML49-B can physically interact with TaIQD23”
t0“Wheat”
The same questions in other papers
loss of function · tolerance: abiotic · Triticum aestivum (wheat)Positive and negative results
4 papers in the same direction as this paper · 8 in the opposite direction
- positive
- positive
- positive
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.