Abstract
OsAAI1 belongs to the HPS_like subfamily of the AAI_LTSS superfamily, yet the molecular mechanism by which it regulates root development under osmotic stress remains unclear. In this study, we found that overexpressing OsAAI1 significantly promoted rice root system growth. Specifically, the primary root length, lateral root number, lateral root density and adventitious root count in the overexpression line (OE19) markedly exceeded those in the wild type (ZH11) and the osaai1 mutant. Consistent with this phenotypic enhancement, the root IAA content in OE19 was substantially higher than in ZH11 and osaai1. We further demonstrated that exogenous IAA application compensated for the root growth defects in the osaai1 mutant. Under PEG-induced osmotic stress, OE19 exhibited the most extensive and densely distributed root system, and exogenous IAA also rescued the inhibited growth of the osaai1 mutant. Mechanistically, we identified an interaction between OsAAI1 and the MADS-box transcription factor OsMADS25. This interaction enhanced the transcriptional expression of two key osmotic stress tolerance genes, LAX1 and OsBAG4. Furthermore, it upregulated the auxin biosynthesis gene OsYUC4 while suppressing the auxin inhibitory factor OsIAA14. This coordinated gene regulation promotes the auxin signalling pathway, thereby stimulating root growth and enhancing osmotic stress tolerance. Collectively, our findings indicate that OsAAI1 and OsMADS25 fulfil critical functions in rice osmotic acclimation by orchestrating downstream gene expression and modulating the auxin pathway.
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Claims 10
Claim · model-written summary
OsAAI1 overexpression increased rice primary root length, lateral root number and lateral root density compared with wild type and mutant.
- Taxon
- rice
Oryza sativae1 - Treatment
- overexpressing OsAAI1e1
- Gene
- OsAAI1e1
- Outcome
- primary root length, lateral root number, lateral root densitye0
- Direction
- exceedede0
- Comparator
- wild type (ZH11) and the osaai1 mutante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“primary root length, lateral root number, lateral root density and adventitious root count in the overexpression line (OE19) markedly exceeded those in the wild type (ZH11) and the osaai1 mutant”
e1“overexpressing OsAAI1 significantly promoted rice root system growth”
Claim · model-written summary
OsAAI1 overexpression increased adventitious root count over wild type and mutant.
- Taxon
- riceOryza sativae1
- Treatment
- overexpressing OsAAI1e1
- Gene
- OsAAI1e1
- Outcome
- adventitious root counte0
- Direction
- exceedede0
- Comparator
- wild type (ZH11) and the osaai1 mutante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“adventitious root count in the overexpression line (OE19) markedly exceeded those in the wild type (ZH11) and the osaai1 mutant”
e1“overexpressing OsAAI1 significantly promoted rice root system growth”
Claim · model-written summary
Root IAA content was higher in OE19 than in ZH11 and osaai1.
- Taxon
- riceOryza sativae1
- Material
- OE19e0
- Outcome
- root IAA contente0
- Direction
- highere0
- Comparator
- ZH11 and osaai1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root IAA content in OE19 was substantially higher than in ZH11 and osaai1”
e1“rice root system growth”
Claim · model-written summary
Exogenous IAA compensated for root growth defects in osaai1.
- Taxon
- riceOryza sativae1
- Material
- osaai1 mutante0
- Treatment
- exogenous IAA applicatione0
- Outcome
- root growth defectse0
- Direction
- compensatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous IAA application compensated for the root growth defects in the osaai1 mutant”
e1“rice root system growth”
Claim · model-written summary
OE19 had the most extensive and dense root system under PEG-induced osmotic stress.
- Taxon
- riceOryza sativae1
- Material
- OE19e0
- Treatment
- PEG-induced osmotic stresse0
- Outcome
- root systeme0
- Direction
- most extensive and densely distributede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under PEG-induced osmotic stress, OE19 exhibited the most extensive and densely distributed root system”
e1“rice root system growth”
Claim · model-written summary
Exogenous IAA rescued inhibited osaai1 growth during PEG-induced osmotic stress.
- Taxon
- riceOryza sativae1
- Material
- osaai1 mutante0
- Treatment
- exogenous IAAe0
- Outcome
- inhibited growthe0
- Direction
- rescuede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under PEG-induced osmotic stress, OE19 exhibited the most extensive and densely distributed root system, and exogenous IAA also rescued the inhibited growth of the osaai1 mutant”
e1“rice root system growth”
Claim · model-written summary
OsAAI1 interacted with OsMADS25.
- Taxon
- riceOryza sativae1
- Gene
- OsAAI1e0
- Outcome
- interactione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“interaction between OsAAI1 and the MADS-box transcription factor OsMADS25”
e1“rice root system growth”
Claim · model-written summary
The OsAAI1–OsMADS25 interaction enhanced LAX1 and OsBAG4 transcription.
- Taxon
- riceOryza sativae2
- Gene
- LAX1 and OsBAG4e0
- Outcome
- transcriptional expressione0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This interaction enhanced the transcriptional expression of two key osmotic stress tolerance genes, LAX1 and OsBAG4”
e1“interaction between OsAAI1 and the MADS-box transcription factor OsMADS25”
e2“rice root system growth”
Claim · model-written summary
The interaction upregulated OsYUC4 expression.
- Taxon
- riceOryza sativae1
- Gene
- OsYUC4e0
- Outcome
- auxin biosynthesis genee0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“upregulated the auxin biosynthesis gene OsYUC4”
e1“rice root system growth”
Claim · model-written summary
The interaction suppressed OsIAA14.
- Taxon
- riceOryza sativae1
- Gene
- OsIAA14e0
- Outcome
- auxin inhibitory factore0
- Direction
- suppressinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suppressing the auxin inhibitory factor OsIAA14”
e1“rice root system growth”
The same questions in other papers
indole-3-acetic acid · growth · Oryza sativa (rice)Positive and negative results
39 papers in the same direction as this paper · 6 in the opposite direction · 1 reporting no difference from an alternative
- positive
- negative
- no effect
43 papers more in this group
See all 47 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.