Abstract
Long non-coding RNA (lncRNA) is a non-coding RNA length over 200 bp, which is abundant in plants. It plays important roles in plant growth, development, and stress response by regulating gene expression or protein function. In the previous study, a fertility-related lncRNA named lncRNA27195 was screened and obtained by transcriptome sequencing from the anther of wheat Photoperiod-thermo Sensitive Genic Male Sterility (PTGMS) line BS366. To investigate the function of lncRNA27195 in wheat, the lncRNA27195 gene and its target gene TaRTS were cloned from BS366. Bioinformatics analysis were performed on TaRTS. The expressions of lncRNA27195 and TaRTS in different tissues and their expression correlation between them were analyzed by qRT-PCR. Meanwhile, the expression patterns of lncRNA27195 and TaRTS under different light and temperature treatments, and methyl jasmonate (MeJA) treatments were investigated. The results showed that the TaRTS gene with 315 bp length, encoded 104 amino acids. Additionally, RTS proteins were only found as anther-specific proteins in gramineae plants. Both lncRNA27195 and TaRTS with a significantly positive correlation were highly expressed in stamens, and revealed different expression patterns in different fertility environments. The results demonstrated that the expression of lncRNA27195 and TaRTS were also regulated by light and temperature. In addition, we found that the appropriate concentration of MeJA could promote the expression of lncRNA27195 and TaRTS while SA could inhibit the expression. The results indicated that under the induction of photoperiod, temperature, and plant hormones, IncRNA27195 positively regulated TaRTS gene expression, resulting in affecting pollen development and male fertility. This study contributed to the mechanism research and production application of PTGMS wheat.
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Claims 8
Claim · model-written summary
TaRTS is 315 bp long and encodes 104 amino acids.
- Taxon
- wheat (Triticum aestivum L.)
Triticum aestivumt0 - Gene
- TaRTSe0
- Outcome
- TaRTS genee0
- Value
- 315 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results showed that the TaRTS gene with 315 bp length, encoded 104 amino acids”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
RTS proteins were found only as anther-specific proteins in gramineae plants.
- Taxon
- wheat (Triticum aestivum L.)Triticum aestivumt0
- Organ / stage
- anthere0
- Gene
- RTS proteinse0
- Outcome
- anther-specific proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RTS proteins were only found as anther-specific proteins in gramineae plants”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
lncRNA27195 and TaRTS expression were significantly positively correlated and high in stamens.
- Taxon
- wheat (Triticum aestivum L.)Triticum aestivumt0
- Organ / stage
- stamense0
- Gene
- lncRNA27195 and TaRTSe0
- Outcome
- lncRNA27195 and TaRTSe0
- Direction
- highlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both lncRNA27195 and TaRTS with a significantly positive correlation were highly expressed in stamens”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
lncRNA27195 and TaRTS showed different expression patterns across fertility environments.
- Taxon
- wheat (Triticum aestivum L.)Triticum aestivumt0
- Gene
- lncRNA27195 and TaRTSe1
- Outcome
- expression patternse0
- Qualifier
- different fertility environmentse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“revealed different expression patterns in different fertility environments”
t0“wheat (Triticum aestivum L.)”
e1“Both lncRNA27195 and TaRTS with a significantly positive correlation were highly expressed in stamens”
Claim · model-written summary
Light and temperature regulated lncRNA27195 and TaRTS expression.
- Taxon
- wheat (Triticum aestivum L.)Triticum aestivumt0
- Treatment
- light and temperaturee0
- Gene
- lncRNA27195 and TaRTSe0
- Outcome
- expression of lncRNA27195 and TaRTSe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of lncRNA27195 and TaRTS were also regulated by light and temperature”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
An appropriate concentration of MeJA promoted lncRNA27195 and TaRTS expression.
- Taxon
- wheat (Triticum aestivum L.)Triticum aestivumt0
- Treatment
- MeJAe0
- Gene
- lncRNA27195 and TaRTSe0
- Outcome
- expression of lncRNA27195 and TaRTSe0
- Direction
- promotee0
- Qualifier
- appropriate concentratione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the appropriate concentration of MeJA could promote the expression of lncRNA27195 and TaRTS”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
SA inhibited lncRNA27195 and TaRTS expression.
- Taxon
- wheat (Triticum aestivum L.)Triticum aestivumt0
- Treatment
- SAe0
- Gene
- lncRNA27195 and TaRTSe1
- Outcome
- expressione0
- Direction
- inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SA could inhibit the expression”
t0“wheat (Triticum aestivum L.)”
e1“the appropriate concentration of MeJA could promote the expression of lncRNA27195 and TaRTS”
Claim · model-written summary
The authors infer that lncRNA27195 positively regulates TaRTS expression under photoperiod, temperature and hormone induction, affecting pollen development and male fertility.
- Taxon
- wheat (Triticum aestivum L.)Triticum aestivumt0
- Gene
- TaRTSe0
- Outcome
- pollen development and male fertilitye0
- Qualifier
- under the induction of photoperiod, temperature, and plant hormonese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“under the induction of photoperiod, temperature, and plant hormones, IncRNA27195 positively regulated TaRTS gene expression, resulting in affecting pollen development and male fertility”
t0“wheat (Triticum aestivum L.)”
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