Paper · 2025 · Frontiers in Environmental Science

Analysis of spectral signatures and vegetation indices to assess the response of faba bean plants to varying zinc levels under sandy soil conditions

Ghada A. Khdery; I. M. El-Metwally; Mohamed S. Shokr; et al.

11
claims
26
verbatim quotes
1
citations
2
research areas
✓
Open access · cc-by

Abstract

Introduction Zinc (Zn) deficiency is a major limiting factor for crop productivity, particularly in sandy soils where nutrient retention and bioavailability are poor. The field experiment was conducted at the Agricultural Experimental Station, Cairo University (Giza, Egypt) during the 2022–2024 winter seasons. This study aimed to analyze the spectral responses of faba bean (Vicia faba L.) under different foliar Zn concentrations (0, 0.5, 1.0, 1.5, and 2.0 g L −1 ZnSO 4 ·7H 2 O) using a Randomized Complete Block Design (RCBD) with three replications, and to identify vegetation indices sensitive to Zn nutrition. Methods Growth traits, biochemical attributes, seed yield, hyperspectral reflectance, and soil spectral properties were integrated to establish robust vegetation–soil indicators. Results and discussion Foliar Zn application increased chlorophyll content by 24.5%, protein by 18.3%, and seed yield by 27.6% compared with the control (p ≤ 0.05). Results revealed that foliar Zn application significantly enhanced chlorophyll content, protein accumulation, soluble sugars, and seed yield, with the optimum response observed at 2.0 g L −1 . Spectral signatures demonstrated that Zn enrichment decreased red reflectance (∼670 nm), sharpened the red-edge slope (700–750 nm), and increased near-infrared reflectance (∼800 nm), indicating improved canopy structure and photosynthetic activity. Vegetation indices such as Normalized Difference Vegetation Index (NDVI), Green NDVI (GNDVI), Photochemical Reflectance Index (PRI), Normalized Difference Red-Edge Index (NDRE), Red-Edge Position (REP), and Modified Chlorophyll Absorption Ratio Index (MCARI) were highly responsive to Zn levels, showing strong correlations with physiological traits and closely aligned with growth and yield performance. Soil-based spectral indices, including the Clay Ratio Index (CRI) and the Normalized Difference Soil Index (NDSI), also differentiated among soil samples, linking soil spectral features with Zn-associated crop responses. These findings confirm that hyperspectral reflectance and derived indices provide reliable, non-destructive tools for monitoring Zn nutrition in faba bean. The integration of spectral traits with agronomic and biochemical data emphasizes the potential of hyperspectral sensing for optimizing micronutrient management in sandy soils. Moreover, the outcomes highlight the role of hyperspectral tools in precision agriculture, enabling site-specific nutrient strategies that enhance productivity, resource-use efficiency, and sustainable food security.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 11

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Foliar Zn application increased chlorophyll content by 24.5% compared with the control.

Taxon
faba beanVicia fabat0
Treatment
Foliar Zn applicatione1
Outcome
chlorophyll contente1
Direction
increasede1
Value
24.5%e1
Qualifier
p ≤ 0.05e1
Comparator
controle1
Setting
Agricultural Experimental Station, Cairo University (Giza, Egypt)e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“faba bean plants”

✓ located verbatim in the source · title, characters 81–97 · show in text ↑
e0

“The field experiment was conducted at the Agricultural Experimental Station, Cairo University (Giza, Egypt) during the 2022–2024 winter seasons”

✓ located verbatim in the source · abstract, characters 167–310 · show in text ↑
e1

“Foliar Zn application increased chlorophyll content by 24.5%, protein by 18.3%, and seed yield by 27.6% compared with the control (p ≤ 0.05)”

✓ located verbatim in the source · abstract, characters 820–960 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Foliar Zn application increased protein content by 18.3% compared with the control.

Taxon
faba beanVicia fabat0
Treatment
Foliar Zn applicatione1
Outcome
proteine1
Direction
increasede1
Value
18.3%e1
Qualifier
p ≤ 0.05e1
Comparator
controle1
Setting
Agricultural Experimental Station, Cairo University (Giza, Egypt)e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“faba bean plants”

✓ located verbatim in the source · title, characters 81–97 · show in text ↑
e0

“The field experiment was conducted at the Agricultural Experimental Station, Cairo University (Giza, Egypt) during the 2022–2024 winter seasons”

✓ located verbatim in the source · abstract, characters 167–310 · show in text ↑
e1

“Foliar Zn application increased chlorophyll content by 24.5%, protein by 18.3%, and seed yield by 27.6% compared with the control (p ≤ 0.05)”

✓ located verbatim in the source · abstract, characters 820–960 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Foliar Zn application increased seed yield by 27.6% compared with the control.

Taxon
faba beanVicia fabat0
Treatment
Foliar Zn applicatione1
Outcome
seed yielde1
Direction
increasede1
Value
27.6%e1
Qualifier
p ≤ 0.05e1
Comparator
controle1
Setting
Agricultural Experimental Station, Cairo University (Giza, Egypt)e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“faba bean plants”

✓ located verbatim in the source · title, characters 81–97 · show in text ↑
e0

“The field experiment was conducted at the Agricultural Experimental Station, Cairo University (Giza, Egypt) during the 2022–2024 winter seasons”

✓ located verbatim in the source · abstract, characters 167–310 · show in text ↑
e1

“Foliar Zn application increased chlorophyll content by 24.5%, protein by 18.3%, and seed yield by 27.6% compared with the control (p ≤ 0.05)”

✓ located verbatim in the source · abstract, characters 820–960 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The optimum response to foliar Zn was observed at 2.0 g L −1.

Taxon
faba beanVicia fabat0
Outcome
optimum responsee2
Value
2.0 g L −1e2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“faba bean plants”

✓ located verbatim in the source · title, characters 81–97 · show in text ↑
e2

“Results revealed that foliar Zn application significantly enhanced chlorophyll content, protein accumulation, soluble sugars, and seed yield, with the optimum response observed at 2.0 g L −1”

✓ located verbatim in the source · abstract, characters 962–1152 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Foliar Zn application significantly enhanced soluble sugars.

Taxon
faba beanVicia fabat0
Treatment
Foliar Zn applicatione2
Outcome
soluble sugarse2
Direction
enhancede2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“faba bean plants”

✓ located verbatim in the source · title, characters 81–97 · show in text ↑
e2

“Results revealed that foliar Zn application significantly enhanced chlorophyll content, protein accumulation, soluble sugars, and seed yield, with the optimum response observed at 2.0 g L −1”

✓ located verbatim in the source · abstract, characters 962–1152 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Zn enrichment decreased red reflectance (∼670 nm).

Taxon
faba beanVicia fabat0
Treatment
Zn enrichmente3
Outcome
red reflectance (∼670 nm)e3
Direction
decreasede3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“faba bean plants”

✓ located verbatim in the source · title, characters 81–97 · show in text ↑
e3

“Spectral signatures demonstrated that Zn enrichment decreased red reflectance (∼670 nm), sharpened the red-edge slope (700–750 nm), and increased near-infrared reflectance (∼800 nm)”

✓ located verbatim in the source · abstract, characters 1155–1336 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Zn enrichment sharpened the red-edge slope (700–750 nm).

Taxon
faba beanVicia fabat0
Treatment
Zn enrichmente3
Outcome
red-edge slope (700–750 nm)e3
Direction
sharpenede3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“faba bean plants”

✓ located verbatim in the source · title, characters 81–97 · show in text ↑
e3

“Spectral signatures demonstrated that Zn enrichment decreased red reflectance (∼670 nm), sharpened the red-edge slope (700–750 nm), and increased near-infrared reflectance (∼800 nm)”

✓ located verbatim in the source · abstract, characters 1155–1336 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Zn enrichment increased near-infrared reflectance (∼800 nm).

Taxon
faba beanVicia fabat0
Treatment
Zn enrichmente3
Outcome
near-infrared reflectance (∼800 nm)e3
Direction
increasede3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“faba bean plants”

✓ located verbatim in the source · title, characters 81–97 · show in text ↑
e3

“Spectral signatures demonstrated that Zn enrichment decreased red reflectance (∼670 nm), sharpened the red-edge slope (700–750 nm), and increased near-infrared reflectance (∼800 nm)”

✓ located verbatim in the source · abstract, characters 1155–1336 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Vegetation indices were highly responsive to Zn levels.

Taxon
faba beanVicia fabat0
Outcome
Vegetation indicese4
Qualifier
highly responsive to Zn levelse4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“faba bean plants”

✓ located verbatim in the source · title, characters 81–97 · show in text ↑
e4

“Vegetation indices such as Normalized Difference Vegetation Index (NDVI), Green NDVI (GNDVI), Photochemical Reflectance Index (PRI), Normalized Difference Red-Edge Index (NDRE), Red-Edge Position (REP), and Modified Chlorophyll Absorption Ratio Index (MCARI) were highly responsive to Zn levels”

✓ located verbatim in the source · abstract, characters 1404–1698 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Vegetation indices showed strong correlations with physiological traits.

Taxon
faba beanVicia fabat0
Outcome
Vegetation indicese4
Qualifier
showing strong correlations with physiological traitse5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“faba bean plants”

✓ located verbatim in the source · title, characters 81–97 · show in text ↑
e4

“Vegetation indices such as Normalized Difference Vegetation Index (NDVI), Green NDVI (GNDVI), Photochemical Reflectance Index (PRI), Normalized Difference Red-Edge Index (NDRE), Red-Edge Position (REP), and Modified Chlorophyll Absorption Ratio Index (MCARI) were highly responsive to Zn levels”

✓ located verbatim in the source · abstract, characters 1404–1698 · show in text ↑
e5

“showing strong correlations with physiological traits”

✓ located verbatim in the source · abstract, characters 1700–1753 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Soil-based spectral indices differentiated among soil samples.

Taxon
faba beanVicia fabat0
Outcome
Soil-based spectral indicese6
Qualifier
differentiated among soil samplese6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“faba bean plants”

✓ located verbatim in the source · title, characters 81–97 · show in text ↑
e6

“Soil-based spectral indices, including the Clay Ratio Index (CRI) and the Normalized Difference Soil Index (NDSI), also differentiated among soil samples”

✓ located verbatim in the source · abstract, characters 1809–1962 · show in text ↑

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zinc atom · physiology: pigments · Vicia faba (faba bean)Results differ

7 papers: 4 positive, 3 negative or no effect

3 papers in the same direction as this paper · 3 in the opposite direction

  1. negative
  2. negative
  3. negative

3 papers more in this group

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zinc atom · yield · Vicia faba (faba bean)Results differ

30 papers: 29 positive, 1 negative or no effect

28 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. positive
  3. negative

26 papers more in this group

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zinc atom · quality: protein · Vicia faba (faba bean)Results agree

9 papers: 9 positive, 0 negative or no effect

8 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

5 papers more in this group

See all 9 papers →

application · quality: protein · Vicia faba (faba bean)Positive and negative results

30 papers: 28 positive, 2 negative or no effect

27 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. no effect
  3. positive

26 papers more in this group

See all 30 papers →

application · yield · Vicia faba (faba bean)Positive and negative results

116 papers: 110 positive, 6 negative or no effect

109 papers in the same direction as this paper · 6 in the opposite direction

  1. positive
  2. negative
  3. no effect

112 papers more in this group

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application · physiology: osmolytes · Vicia faba (faba bean)Results agree

9 papers: 9 positive, 0 negative or no effect

8 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

5 papers more in this group

See all 9 papers →

application · physiology: pigments · Vicia faba (faba bean)Results agree

23 papers: 23 positive, 0 negative or no effect

22 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

19 papers more in this group

See all 23 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.