Abstract
Zinc (Zn) is often of deficient in paddy soils, and optimizing its application is crucial for improving maize productivity in intensive rice–maize cropping systems. This study aimed to develop practical Zn fertilizer strategies suitable for paddy soils with varying pH levels, thereby improving nutrient management and understanding of soil microbial responses. Field experiments were conducted during the 2020–2021 dry seasons at three sites: Chon Daen (pH 5.8), Noen Maprang (pH 6.7), and Lom Sak (pH 7.8). A two-factorial randomized complete block design with four replications was used, including four ZnSO4·H2O rates (0, 1.5, 3, and 6 times the DTPA-extractable Zn in soil) and two hybrid maize varieties, Suwan 5731 and Suwan 5819. Results showed that at Chon Daen, Zn application significantly enhanced shoot Zn uptake and soil Zn concentration, with SW5819 exhibiting greater Zn efficiency and biomass production. At Noen Maprang, Zn application did not significantly affect dry matter, while, at Lom Sak, Zn responses were moderate, though SW5819 maintained better growth and Zn uptake. Across sites, maize Zn efficiency was highest under acidic conditions and in SW5819. Soil microbial communities remained largely unaffected by Zn fertilization and were primarily influenced by soil pH, with Proteobacteria, Crenarchaeota, and Ascomycota dominating bacterial, archaeal, and fungal groups, respectively. These findings support the feasibility of Zn fertilization strategies to enhance both crop productivity and nutritional quality without altering the microbial community composition.
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Claims 11
Claim · model-written summary
At Chon Daen, Zn application significantly enhanced shoot Zn uptake and soil Zn concentration.
- Treatment
- Zn applicatione1
- Outcome
- shoot Zn uptake and soil Zn concentratione1
- Direction
- significantly enhancede1
- Setting
- Chon Daene1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Effect of Zinc Application on Maize Dry Matter”
e1“at Chon Daen, Zn application significantly enhanced shoot Zn uptake and soil Zn concentration”
Claim · model-written summary
At Chon Daen, SW5819 showed greater Zn efficiency and biomass production.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Effect of Zinc Application on Maize Dry Matter”
e1“at Chon Daen, Zn application significantly enhanced shoot Zn uptake and soil Zn concentration”
e2“with SW5819 exhibiting greater Zn efficiency and biomass production”
Claim · model-written summary
At Noen Maprang, Zn application did not significantly affect maize dry matter.
- Treatment
- Zn applicatione3
- Outcome
- dry mattere3
- Direction
- did not significantly affecte3
- Setting
- Noen Maprange3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Effect of Zinc Application on Maize Dry Matter”
e3“At Noen Maprang, Zn application did not significantly affect dry matter”
Claim · model-written summary
At Lom Sak, maize responses to Zn were moderate.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Effect of Zinc Application on Maize Dry Matter”
e4“at Lom Sak, Zn responses were moderate”
Claim · model-written summary
At Lom Sak, SW5819 maintained better growth and Zn uptake.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Effect of Zinc Application on Maize Dry Matter”
e4“at Lom Sak, Zn responses were moderate”
e5“though SW5819 maintained better growth and Zn uptake”
Claim · model-written summary
Across sites, maize Zn efficiency was highest under acidic conditions.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Effect of Zinc Application on Maize Dry Matter”
e6“Across sites, maize Zn efficiency was highest under acidic conditions and in SW5819”
Claim · model-written summary
Across sites, maize Zn efficiency was highest in SW5819.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Effect of Zinc Application on Maize Dry Matter”
e6“Across sites, maize Zn efficiency was highest under acidic conditions and in SW5819”
Claim · model-written summary
Soil microbial communities remained largely unaffected by Zn fertilization.
- Treatment
- Zn fertilizatione7
- Outcome
- Soil microbial communitiese7
- Direction
- remained largely unaffectede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Effect of Zinc Application on Maize Dry Matter”
e7“Soil microbial communities remained largely unaffected by Zn fertilization and were primarily influenced by soil pH”
Claim · model-written summary
Soil microbial communities were primarily influenced by soil pH.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Effect of Zinc Application on Maize Dry Matter”
e7“Soil microbial communities remained largely unaffected by Zn fertilization and were primarily influenced by soil pH”
Claim · model-written summary
Proteobacteria, Crenarchaeota and Ascomycota dominated the bacterial, archaeal and fungal groups in the soil.
- Outcome
- bacterial, archaeal, and fungal groupse1
- Qualifier
- Proteobacteria, Crenarchaeota, and Ascomycotae1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Effect of Zinc Application on Maize Dry Matter”
e1“Proteobacteria, Crenarchaeota, and Ascomycota dominating bacterial, archaeal, and fungal groups, respectively”
Claim · model-written summary
Zn fertilization strategies are feasible to enhance maize productivity and nutritional quality without altering microbial community composition.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Effect of Zinc Application on Maize Dry Matter”
e1“These findings support the feasibility of Zn fertilization strategies to enhance both crop productivity and nutritional quality without altering the microbial community composition”
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