Paper · 2026 · Horticulturae

Microbial Biostimulants and Their Combinations Alter Phytochemical Quality of Strawberry Fruits in a Soilless Cultivation System

José Luís Trevizan Chiomento; Nícolas Alberton Mandelli; Ana Júlia Schroeder Ely; et al.

9
claims
17
verbatim quotes
1
citations
3
research areas
✓
Open access · cc-by

Abstract

This study investigated the effect of arbuscular mycorrhizal fungi (AMF), Trichoderma harzianum, and their combinations on yield and quality of strawberry fruits in a soilless cultivation system. Six treatments were applied, control (no biostimulants), T. harzianum (TH), Claroideoglomus etunicatum (CE), a multispecies mycorrhizal community (IP CS), TH + CE, and TH + IP CS, arranged in a randomized block design with four replicates. While total fruit yield was not significantly affected, the application of T. harzianum, either alone or in combination with AMF, enhanced cumulative fruit production. The use of C. etunicatum improved sugar content and the sugar/acid ratio by 28% and 31%, respectively, compared to the control. Biostimulant treatments also increased total phytochemical content, particularly with the multispecies inoculant IP CS (increased anthocyanin content by 39% compared to the control) and the combinations TH + CE (flavonoid content 41% higher than the control) and TH + IP CS (flavonoid content 39% higher than the control). Multivariate analysis grouped the treatments into two groups, with the control (no biostimulants) forming a distinct group. In conclusion, biostimulation of ‘San Andreas’ strawberry plants improved fruit quality without significantly increasing yield. The combined use of AMF and T. harzianum is proposed as a sustainable strategy for enhancing fruit quality in soilless strawberry cultivation systems.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

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Claims 9

Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Biostimulants did not significantly affect total fruit yield.

Taxon
StrawberryFragaria ×ananassat0
Outcome
total fruit yielde0
Direction
not significantly affectede0

Not reportedTreatmentComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“total fruit yield was not significantly affected”

✓ located verbatim in the source · abstract, characters 442–490 · show in text ↑
t0

“Strawberry Fruits”

✓ located verbatim in the source · title, characters 78–95 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

T. harzianum alone or with AMF enhanced cumulative fruit production.

Taxon
StrawberryFragaria ×ananassat0
Treatment
application of T. harzianum, either alone or in combination with AMFe0
Interactor
T. harzianumTrichoderma harzianume0symbiont
Outcome
cumulative fruit productione0
Direction
enhancede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · InteractorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“application of T. harzianum, either alone or in combination with AMF, enhanced cumulative fruit production”

✓ located verbatim in the source · abstract, characters 496–602 · show in text ↑
t0

“Strawberry Fruits”

✓ located verbatim in the source · title, characters 78–95 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

C. etunicatum raised sugar content by 28% versus control.

Taxon
StrawberryFragaria ×ananassat0
Treatment
C. etunicatume0
Interactor
C. etunicatumEntrophospora etunicatae0symbiont
Outcome
sugar contente0
Direction
improvede0
Value
28%e0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗
Entrophospora etunicata
Entrophospora etunicata · InteractorIeatbeansss · CC0 · Wikimedia Commons ↗

Verbatim

e0

“use of C. etunicatum improved sugar content and the sugar/acid ratio by 28% and 31%, respectively, compared to the control”

✓ located verbatim in the source · abstract, characters 608–730 · show in text ↑
t0

“Strawberry Fruits”

✓ located verbatim in the source · title, characters 78–95 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

C. etunicatum raised sugar/acid ratio by 31% versus control.

Taxon
StrawberryFragaria ×ananassat0
Treatment
C. etunicatume0
Interactor
C. etunicatumEntrophospora etunicatae0symbiont
Outcome
sugar/acid ratioe0
Direction
improvede0
Value
31%e0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗
Entrophospora etunicata
Entrophospora etunicata · InteractorIeatbeansss · CC0 · Wikimedia Commons ↗

Verbatim

e0

“use of C. etunicatum improved sugar content and the sugar/acid ratio by 28% and 31%, respectively, compared to the control”

✓ located verbatim in the source · abstract, characters 608–730 · show in text ↑
t0

“Strawberry Fruits”

✓ located verbatim in the source · title, characters 78–95 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

IP CS increased anthocyanin content by 39% versus control.

Taxon
StrawberryFragaria ×ananassat0
Treatment
IP CSe0
Interactor
IP CSe0symbiont
Outcome
anthocyanin contente0
Direction
increasede0
Value
39%e0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“multispecies inoculant IP CS (increased anthocyanin content by 39% compared to the control)”

✓ located verbatim in the source · abstract, characters 822–913 · show in text ↑
t0

“Strawberry Fruits”

✓ located verbatim in the source · title, characters 78–95 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

TH + CE raised flavonoid content by 41% versus control.

Taxon
StrawberryFragaria ×ananassat0
Treatment
TH + CEe0
Outcome
flavonoid contente0
Direction
highere0
Value
41%e0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“TH + CE (flavonoid content 41% higher than the control)”

✓ located verbatim in the source · abstract, characters 935–990 · show in text ↑
t0

“Strawberry Fruits”

✓ located verbatim in the source · title, characters 78–95 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

TH + IP CS raised flavonoid content by 39% versus control.

Taxon
StrawberryFragaria ×ananassat0
Treatment
TH + IP CSe0
Outcome
flavonoid contente0
Direction
highere0
Value
39%e0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“TH + IP CS (flavonoid content 39% higher than the control)”

✓ located verbatim in the source · abstract, characters 995–1053 · show in text ↑
t0

“Strawberry Fruits”

✓ located verbatim in the source · title, characters 78–95 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Multivariate analysis separated control from biostimulant treatments.

Taxon
StrawberryFragaria ×ananassat0
Outcome
control (no biostimulants) forming a distinct groupe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Multivariate analysis grouped the treatments into two groups, with the control (no biostimulants) forming a distinct group”

✓ located verbatim in the source · abstract, characters 1055–1177 · show in text ↑
t0

“Strawberry Fruits”

✓ located verbatim in the source · title, characters 78–95 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

Authors propose combined AMF and T. harzianum for fruit quality in soilless strawberry cultivation.

Taxon
strawberryFragaria ×ananassae0
Outcome
combined use of AMF and T. harzianume0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“combined use of AMF and T. harzianum is proposed as a sustainable strategy for enhancing fruit quality in soilless strawberry cultivation systems”

✓ located verbatim in the source · abstract, characters 1311–1456 · show in text ↑

Entrophospora etunicata · quality: phytochemicals · Fragaria x ananassa (strawberry)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Trichoderma harzianum · yield · Fragaria x ananassa (strawberry)Results agree

6 papers: 6 positive, 0 negative or no effect

5 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

2 papers more in this group

See all 6 papers →

mycorrhiza · yield · Fragaria x ananassa (strawberry)Results differ

34 papers: 30 positive, 4 negative or no effect

29 papers in the same direction as this paper · 4 in the opposite direction

  1. positive
  2. positive
  3. no effect

30 papers more in this group

See all 34 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.