Abstract
Sucrose-binding proteins (SBPs), a class of proteins in plants, are involved in sucrose transport and utilization, and play a vital contribution in regulating plant growth and development. As demonstrated by prior studies, in plants such as soybean and tobacco, SBP-mediated sucrose transport is crucial for both plant growth and development and carbohydrate partitioning. However, in comparison to the extensive research on the function of SBP in angiosperms, there is currently a lack of in-depth studies on the specific role of SBPs in gymnosperms. In the gymnosperm Torreya grandis , we identified that TgSBP1 may be involved in the cell expansion of its cones by mediating sucrose accumulation. In this study, we found that during cone development in T. grandis , the expression of TgSBP1 showed a significant upward trend. Heterologous expression in tomatoes and transient overexpression in T. grandis cones indicated that TgSBP1 positively modulated the expansion of cone cells. In contrast, transient silencing of TgSBP1 significantly diminished sucrose accumulation and reduced cell size. Furthermore, the WRKY transcription factor TgWRKY25 was identified to participate in regulating TgSBP1 expression. Further functional analysis revealed that TgWRKY25 directly bound to the promoter of TgSBP1 and upregulate its expression, which increased sucrose content in cones and led to a significant enlargement of cell size. Taken together, these findings indicate that TgWRKY25 modulates sucrose level by upregulating TgSBP1 expression, thereby promoting cone cell expansion in T. grandis .
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Claims 7
Claim · model-written summary
TgSBP1 expression rose significantly during cone development in Torreya grandis.
- Taxon
- Torreya grandis
Torreya grandist0 - Organ / stage
- cone developmente1
- Gene
- TgSBP1e1
- Outcome
- expressione1
- Direction
- upward trende1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Torreya grandis”
e1“during cone development in T. grandis , the expression of TgSBP1 showed a significant upward trend”
Claim · model-written summary
Transient overexpression of TgSBP1 in T. grandis cones positively modulated cone cell expansion.
- Taxon
- Torreya grandisTorreya grandist0
- Treatment
- transient overexpressione1
- Gene
- TgSBP1e1
- Outcome
- expansion of cone cellse1
- Direction
- positively modulatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Torreya grandis”
e1“Heterologous expression in tomatoes and transient overexpression in T. grandis cones indicated that TgSBP1 positively modulated the expansion of cone cells”
Claim · model-written summary
Heterologous expression of TgSBP1 in tomatoes positively modulated cell expansion.
- Taxon
- tomatoes
Solanum lycopersicume1 - Treatment
- Heterologous expressione1
- Gene
- TgSBP1e1
- Outcome
- expansion of cone cellse1
- Direction
- positively modulatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Heterologous expression in tomatoes and transient overexpression in T. grandis cones indicated that TgSBP1 positively modulated the expansion of cone cells”
Claim · model-written summary
Transient silencing of TgSBP1 significantly diminished sucrose accumulation and reduced cell size in T. grandis.
- Taxon
- Torreya grandisTorreya grandist0
- Treatment
- transient silencinge1
- Gene
- TgSBP1e1
- Outcome
- sucrose accumulation and reduced cell sizee1
- Direction
- diminishede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Torreya grandis”
e1“transient silencing of TgSBP1 significantly diminished sucrose accumulation and reduced cell size”
Claim · model-written summary
TgWRKY25 directly bound the TgSBP1 promoter and upregulated its expression.
- Taxon
- Torreya grandisTorreya grandist0
- Gene
- TgWRKY25e1
- Outcome
- directly bound to the promoter of TgSBP1 and upregulate its expressione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Torreya grandis”
e1“TgWRKY25 directly bound to the promoter of TgSBP1 and upregulate its expression”
Claim · model-written summary
Through TgSBP1 upregulation, TgWRKY25 increased sucrose content in cones and led to a significant enlargement of cell size.
- Taxon
- Torreya grandisTorreya grandist0
- Organ / stage
- conese1
- Gene
- TgWRKY25e1
- Outcome
- sucrose content in cones and led to a significant enlargement of cell sizee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Torreya grandis”
e1“TgWRKY25 directly bound to the promoter of TgSBP1 and upregulate its expression, which increased sucrose content in cones and led to a significant enlargement of cell size”
Claim · model-written summary
The authors conclude that TgWRKY25 modulates sucrose level by upregulating TgSBP1 expression, thereby promoting cone cell expansion in T. grandis.
- Taxon
- Torreya grandisTorreya grandist0
- Gene
- TgWRKY25e1
- Outcome
- cone cell expansione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Torreya grandis”
e1“TgWRKY25 modulates sucrose level by upregulating TgSBP1 expression, thereby promoting cone cell expansion”