Abstract
ABSTRACT Low temperature as one of the most important abiotic stresses has caused serious wheat production loss worldwide. We identified TaWRKY115 affecting cold tolerance in wheat by integration of genome-wide association study and RNA sequencing. Overexpression and CRISPR/Cas9-mediated gene-editing revealed TaWRKY115 positively modulating cold tolerance in wheat. Transcription factor TaSP1 negatively regulates the cold tolerance of wheat by binding to the promoter of TaWRKY115 in cold-sensitive haplotypes. TaWRKY115 inhibited TaMYB4 at both protein and transcription levels. BSMV-mediated silenced wheat plants and EMS mutants of TaMYB4 possessed increased wheat cold tolerance. We further revealed that TaMYB4 repressed expressions of the TaCBF family through binding to their promoters. BSMV-mediated silenced wheat plants and EMS mutants of TaCBF12d exhibited decreased cold tolerance in wheat. This study brought forth a new model TaSP1-TaWRKY115-TaMYB4, regulating wheat cold tolerance through the TaCBF pathway and provided valuable cold tolerance genes for wheat breeding programs.
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Claims 11
Claim · model-written summary
Integrated association and RNA-seq analysis identified TaWRKY115 as affecting wheat cold tolerance.
- Taxon
- wheat
Triticum aestivume0 - Gene
- TaWRKY115e0
- Outcome
- cold tolerancee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified TaWRKY115 affecting cold tolerance in wheat by integration of genome-wide association study and RNA sequencing”
Claim · model-written summary
Overexpression and editing showed TaWRKY115 positively modulates wheat cold tolerance.
- Taxon
- wheatTriticum aestivume0
- Treatment
- Overexpression and CRISPR/Cas9-mediated gene-editinge0
- Gene
- TaWRKY115e0
- Outcome
- cold tolerancee0
- Direction
- positivelye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression and CRISPR/Cas9-mediated gene-editing revealed TaWRKY115 positively modulating cold tolerance in wheat”
Claim · model-written summary
TaSP1 negatively regulates wheat cold tolerance by binding the TaWRKY115 promoter in cold-sensitive haplotypes.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaSP1e0
- Outcome
- cold tolerancee0
- Direction
- negativelye0
- Qualifier
- binding to the promoter of TaWRKY115 in cold-sensitive haplotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaSP1 negatively regulates the cold tolerance of wheat by binding to the promoter of TaWRKY115 in cold-sensitive haplotypes”
Claim · model-written summary
TaWRKY115 inhibited TaMYB4 at protein level.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaWRKY115 inhibited TaMYB4 at both protein and transcription levels”
t0“wheat”
Claim · model-written summary
TaWRKY115 inhibited TaMYB4 at transcription level.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaWRKY115e0
- Outcome
- TaMYB4e0
- Direction
- inhibitede0
- Qualifier
- transcriptione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaWRKY115 inhibited TaMYB4 at both protein and transcription levels”
t0“wheat”
Claim · model-written summary
Silencing TaMYB4 increased wheat cold tolerance.
- Taxon
- wheatTriticum aestivume0
- Treatment
- BSMV-mediated silencede0
- Gene
- TaMYB4e0
- Outcome
- wheat cold tolerancee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BSMV-mediated silenced wheat plants and EMS mutants of TaMYB4 possessed increased wheat cold tolerance”
Claim · model-written summary
TaMYB4 EMS mutants had increased wheat cold tolerance.
- Taxon
- wheatTriticum aestivume0
- Material
- EMS mutants of TaMYB4e0
- Gene
- TaMYB4e0
- Outcome
- wheat cold tolerancee0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EMS mutants of TaMYB4 possessed increased wheat cold tolerance”
Claim · model-written summary
TaMYB4 repressed TaCBF family expression by binding their promoters.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaMYB4e0
- Outcome
- expressions of the TaCBF familye0
- Direction
- repressede0
- Qualifier
- binding to their promoterse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaMYB4 repressed expressions of the TaCBF family through binding to their promoters”
t0“wheat”
Claim · model-written summary
Silencing TaCBF12d decreased wheat cold tolerance.
- Taxon
- wheatTriticum aestivume0
- Treatment
- BSMV-mediated silencede0
- Gene
- TaCBF12de0
- Outcome
- cold tolerancee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BSMV-mediated silenced wheat plants and EMS mutants of TaCBF12d exhibited decreased cold tolerance in wheat”
Claim · model-written summary
TaCBF12d EMS mutants had decreased wheat cold tolerance.
- Taxon
- wheatTriticum aestivume0
- Material
- EMS mutants of TaCBF12de0
- Gene
- TaCBF12de0
- Outcome
- cold tolerancee0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EMS mutants of TaCBF12d exhibited decreased cold tolerance in wheat”
Claim · model-written summary
The authors propose a TaSP1-TaWRKY115-TaMYB4 regulatory model acting through TaCBF in wheat cold tolerance.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaSP1-TaWRKY115-TaMYB4e0
- Outcome
- cold tolerancee0
- Qualifier
- through the TaCBF pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a new model TaSP1-TaWRKY115-TaMYB4, regulating wheat cold tolerance through the TaCBF pathway”
The same questions in other papers
loss of function · tolerance: abiotic · Triticum aestivum (wheat)Positive and negative results
7 papers in the same direction as this paper · 5 in the opposite direction
- negative
- negative
- negative
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.