Paper · 2026 · AMB Express

Effects of growth stage and moisture content on the fermentation profile, chemical composition, and bacterial community structure of triticale silage

Mingze Lan; Maoya Li; Yao Lei; et al.

10
claims
23
verbatim quotes
1
citations
2
research areas
✓
Open access · cc-by-nc-nd

Abstract

Triticale (× Triticosecale wittmack) is a high-yielding and nutritionally balanced forage crop with considerable potential for silage production. This study examined the effects of growth stage and moisture content on the fermentation quality and bacterial community composition of triticale silage by harvesting at heading and dough stages and ensiling at 60%, 70%, and 80% moisture content for 60 days. Results showed that both factors significantly affected nutritional components, pH, lactic acid, acetic acid, and ammonia nitrogen concentrations; silage from the dough stage generally had higher lactic acid levels and lower pH and ammonia nitrogen, indicating more favorable fermentation than that from the heading stage. Ensiling at 70% moisture resulted in higher crude protein and water-soluble carbohydrate contents, lower neutral and acid detergent fiber concentrations, greater lactic acid production, and lower pH, corresponding to improved fermentation characteristics. Bacterial community analysis revealed that 70% moisture enhanced the relative abundances of Firmicutes and Lactiplantibacillus plantarum and reduced Proteobacteria and other undesirable microorganisms. In summary, harvesting triticale at the dough stage with a controlled moisture content of 70% contributes to improved fermentation quality and a beneficial microbial community, providing a theoretical basis and technical support for high-quality triticale silage production in sustainable livestock agriculture.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 10

Observationobservedoriginal resultmixedsignificant (as stated)measured

Claim · model-written summary

Growth stage and moisture content significantly affected silage nutritional components, pH and acid and ammonia concentrations.

Taxon
triticale×Triticosecalet0
Outcome
nutritional components, pH, lactic acid, acetic acid, and ammonia nitrogen concentrationse0
Qualifier
growth stage and moisture contente1

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

x Triticosecale
×Triticosecale · Taxon · picture from its Wikimedia Commons categoryMiquel Pujol Palol · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“both factors significantly affected nutritional components, pH, lactic acid, acetic acid, and ammonia nitrogen concentrations”

✓ located verbatim in the source · abstract, characters 425–550 · show in text ↑
e1

“growth stage and moisture content”

✓ located verbatim in the source · abstract, characters 181–214 · show in text ↑
t0

“triticale silage”

✓ located verbatim in the source · title, characters 133–149 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Dough-stage silage had higher lactic acid than heading-stage silage.

Taxon
triticale×Triticosecalet0
Organ / stage
dough stagee0
Outcome
lactic acid levelse0
Direction
highere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

x Triticosecale
×Triticosecale · Taxon · picture from its Wikimedia Commons categoryMiquel Pujol Palol · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“silage from the dough stage generally had higher lactic acid levels and lower pH and ammonia nitrogen”

✓ located verbatim in the source · abstract, characters 552–653 · show in text ↑
t0

“triticale silage”

✓ located verbatim in the source · title, characters 133–149 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Dough-stage silage had lower pH and ammonia nitrogen than heading-stage silage.

Taxon
triticale×Triticosecalet0
Organ / stage
dough stagee0
Outcome
pH and ammonia nitrogene0
Direction
lowere0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

x Triticosecale
×Triticosecale · Taxon · picture from its Wikimedia Commons categoryMiquel Pujol Palol · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“silage from the dough stage generally had higher lactic acid levels and lower pH and ammonia nitrogen”

✓ located verbatim in the source · abstract, characters 552–653 · show in text ↑
t0

“triticale silage”

✓ located verbatim in the source · title, characters 133–149 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ensiling at 70% moisture increased crude protein and water-soluble carbohydrates.

Taxon
triticale×Triticosecalet0
Treatment
Ensiling at 70% moisturee0
Outcome
crude protein and water-soluble carbohydrate contentse0
Direction
highere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

x Triticosecale
×Triticosecale · Taxon · picture from its Wikimedia Commons categoryMiquel Pujol Palol · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Ensiling at 70% moisture resulted in higher crude protein and water-soluble carbohydrate contents”

✓ located verbatim in the source · abstract, characters 728–825 · show in text ↑
t0

“triticale silage”

✓ located verbatim in the source · title, characters 133–149 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ensiling at 70% moisture lowered neutral and acid detergent fiber.

Taxon
triticale×Triticosecalet0
Treatment
Ensiling at 70% moisturee1
Outcome
neutral and acid detergent fiber concentrationse0
Direction
lowere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

x Triticosecale
×Triticosecale · Taxon · picture from its Wikimedia Commons categoryMiquel Pujol Palol · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“lower neutral and acid detergent fiber concentrations”

✓ located verbatim in the source · abstract, characters 827–880 · show in text ↑
e1

“Ensiling at 70% moisture”

✓ located verbatim in the source · abstract, characters 728–752 · show in text ↑
t0

“triticale silage”

✓ located verbatim in the source · title, characters 133–149 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ensiling at 70% moisture increased lactic acid production.

Taxon
triticale×Triticosecalet0
Treatment
Ensiling at 70% moisturee1
Outcome
lactic acid productione0
Direction
greatere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

x Triticosecale
×Triticosecale · Taxon · picture from its Wikimedia Commons categoryMiquel Pujol Palol · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“greater lactic acid production”

✓ located verbatim in the source · abstract, characters 882–912 · show in text ↑
e1

“Ensiling at 70% moisture”

✓ located verbatim in the source · abstract, characters 728–752 · show in text ↑
t0

“triticale silage”

✓ located verbatim in the source · title, characters 133–149 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ensiling at 70% moisture lowered pH.

Taxon
triticale×Triticosecalet0
Treatment
Ensiling at 70% moisturee1
Outcome
pHe0
Direction
lowere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

x Triticosecale
×Triticosecale · Taxon · picture from its Wikimedia Commons categoryMiquel Pujol Palol · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“greater lactic acid production, and lower pH”

✓ located verbatim in the source · abstract, characters 882–926 · show in text ↑
e1

“Ensiling at 70% moisture”

✓ located verbatim in the source · abstract, characters 728–752 · show in text ↑
t0

“triticale silage”

✓ located verbatim in the source · title, characters 133–149 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

At 70% moisture Firmicutes and Lactiplantibacillus plantarum relative abundances increased.

Taxon
triticale×Triticosecalet0
Treatment
70% moisturee0
Outcome
relative abundances of Firmicutes and Lactiplantibacillus plantarume0
Direction
enhancede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

x Triticosecale
×Triticosecale · Taxon · picture from its Wikimedia Commons categoryMiquel Pujol Palol · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“70% moisture enhanced the relative abundances of Firmicutes and Lactiplantibacillus plantarum”

✓ located verbatim in the source · abstract, characters 1027–1120 · show in text ↑
t0

“triticale silage”

✓ located verbatim in the source · title, characters 133–149 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

At 70% moisture Proteobacteria and undesirable microorganisms declined.

Taxon
triticale×Triticosecalet0
Treatment
70% moisturee0
Outcome
Proteobacteria and other undesirable microorganismse0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

x Triticosecale
×Triticosecale · Taxon · picture from its Wikimedia Commons categoryMiquel Pujol Palol · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“70% moisture enhanced the relative abundances of Firmicutes and Lactiplantibacillus plantarum and reduced Proteobacteria and other undesirable microorganisms”

✓ located verbatim in the source · abstract, characters 1027–1184 · show in text ↑
t0

“triticale silage”

✓ located verbatim in the source · title, characters 133–149 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors recommend dough-stage harvest and 70% moisture for improved silage fermentation and microbes.

Taxon
triticale×Triticosecalee0
Outcome
harvesting triticale at the dough stage with a controlled moisture content of 70%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

x Triticosecale
×Triticosecale · Taxon · picture from its Wikimedia Commons categoryMiquel Pujol Palol · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“harvesting triticale at the dough stage with a controlled moisture content of 70% contributes to improved fermentation quality and a beneficial microbial community”

✓ located verbatim in the source · abstract, characters 1198–1361 · show in text ↑

processing · quality: grain · x Triticosecale (triticale)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
See all 3 papers →

processing · yield · x Triticosecale (triticale)Positive and negative results

3 papers: 1 positive, 2 negative or no effect

2 in the opposite direction

  1. negative
  2. negative
See all 3 papers →

processing · quality: protein · x Triticosecale (triticale)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.