Paper · 2026 · BMC Plant Biology

Biofortifying sunflower with Zinc (Zn) aminochelates: a promising strategy for calcareous soil conditions

Mina Alipourbabadi; Mojtaba Norouzi Masir; Abdolamir Moezzi; et al.

8
claims
15
verbatim quotes
0
citations
2
research areas
✓
Open access · cc-by-nc-nd

Abstract

BACKGROUND: Zinc (Zn) deficiency in calcareous soils limits crop productivity and restricts nutritional quality in edible plant products. AIMS: This study assessed the effectiveness of Zn-aminochelates, compared with conventional ZnSO₄, applied through various application methods, in improving physiological traits, growth, yield, and seed Zn biofortification in sunflower (Helianthus annuus L.) under calcareous soil conditions. METHODS: A field experiment was conducted using a randomized complete block design with three replications. Zn(glycine)₂ [Zn(Gly)₂], Zn(methionine)₂ [Zn(Met)₂], and ZnSO₄ were applied through seed priming (2% solution), fertigation (4 L ha⁻¹ for Zn-aminochelates and 40 kg ha⁻¹ for ZnSO₄), and foliar spray (0.2% w/v, 2 L ha⁻¹ per application) at key growth stages. Control plots received untreated seeds or water. RESULTS: Compared with ZnSO₄, Zn-aminochelates significantly improved seed yield, biomass production, and Zn accumulation. Foliar application of [Zn(Met)₂] was the most effective treatment, increasing seed yield by approximately 85%, oil yield by about 2.5-fold, and seed Zn uptake by nearly twofold, while also enhancing physiological traits, antioxidant enzyme activities, and protein accumulation. The N/S ratio decreased concomitantly with increased seed oil content. CONCLUSIONS: The superior performance of methionine-based Zn chelates is likely linked to their role in sulfur metabolism, enhanced Zn mobility, and improved antioxidant regulation. Overall, foliar application of Zn-aminochelates represents an effective and practical agronomic biofortification strategy for improving productivity and nutritional quality of sunflower grown on calcareous soils.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 8

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Zn-aminochelates significantly improved seed yield, biomass production and Zn accumulation compared with ZnSO4.

Taxon
sunflowerHelianthus annuuse1
Treatment
Zn-aminochelatese2
Outcome
seed yield, biomass production, and Zn accumulatione2
Direction
improvede2
Comparator
ZnSO₄e2
Setting
calcareous soil conditionse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e1

“This study assessed the effectiveness of Zn-aminochelates, compared with conventional ZnSO₄, applied through various application methods, in improving physiological traits, growth, yield, and seed Zn biofortification in sunflower (Helianthus annuus L.) under calcareous soil conditions”

✓ located verbatim in the source · abstract, characters 144–429 · show in text ↑
e2

“Compared with ZnSO₄, Zn-aminochelates significantly improved seed yield, biomass production, and Zn accumulation”

✓ located verbatim in the source · abstract, characters 855–967 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar application of Zn(Met)2 was the most effective treatment, increasing seed yield by approximately 85%.

Taxon
sunflowerHelianthus annuuse1
Treatment
Foliar application of [Zn(Met)₂]e3
Outcome
seed yielde3
Direction
increasinge3
Value
approximately 85%e3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e1

“This study assessed the effectiveness of Zn-aminochelates, compared with conventional ZnSO₄, applied through various application methods, in improving physiological traits, growth, yield, and seed Zn biofortification in sunflower (Helianthus annuus L.) under calcareous soil conditions”

✓ located verbatim in the source · abstract, characters 144–429 · show in text ↑
e3

“Foliar application of [Zn(Met)₂] was the most effective treatment, increasing seed yield by approximately 85%, oil yield by about 2.5-fold, and seed Zn uptake by nearly twofold, while also enhancing physiological traits, antioxidant enzyme activities, and protein accumulation”

✓ located verbatim in the source · abstract, characters 969–1245 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar Zn(Met)2 increased oil yield about 2.5-fold.

Taxon
sunflowerHelianthus annuuse1
Treatment
Foliar application of [Zn(Met)₂]e3
Outcome
oil yielde3
Direction
increasinge3
Value
about 2.5-folde3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e1

“This study assessed the effectiveness of Zn-aminochelates, compared with conventional ZnSO₄, applied through various application methods, in improving physiological traits, growth, yield, and seed Zn biofortification in sunflower (Helianthus annuus L.) under calcareous soil conditions”

✓ located verbatim in the source · abstract, characters 144–429 · show in text ↑
e3

“Foliar application of [Zn(Met)₂] was the most effective treatment, increasing seed yield by approximately 85%, oil yield by about 2.5-fold, and seed Zn uptake by nearly twofold, while also enhancing physiological traits, antioxidant enzyme activities, and protein accumulation”

✓ located verbatim in the source · abstract, characters 969–1245 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar Zn(Met)2 increased seed Zn uptake nearly twofold.

Taxon
sunflowerHelianthus annuuse1
Treatment
Foliar application of [Zn(Met)₂]e3
Outcome
seed Zn uptakee3
Direction
increasinge3
Value
nearly twofolde3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e1

“This study assessed the effectiveness of Zn-aminochelates, compared with conventional ZnSO₄, applied through various application methods, in improving physiological traits, growth, yield, and seed Zn biofortification in sunflower (Helianthus annuus L.) under calcareous soil conditions”

✓ located verbatim in the source · abstract, characters 144–429 · show in text ↑
e3

“Foliar application of [Zn(Met)₂] was the most effective treatment, increasing seed yield by approximately 85%, oil yield by about 2.5-fold, and seed Zn uptake by nearly twofold, while also enhancing physiological traits, antioxidant enzyme activities, and protein accumulation”

✓ located verbatim in the source · abstract, characters 969–1245 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar Zn(Met)2 enhanced physiological traits, antioxidant enzyme activities and protein accumulation.

Taxon
sunflowerHelianthus annuuse1
Treatment
Foliar application of [Zn(Met)₂]e3
Outcome
physiological traits, antioxidant enzyme activities, and protein accumulatione3
Direction
enhancinge3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e1

“This study assessed the effectiveness of Zn-aminochelates, compared with conventional ZnSO₄, applied through various application methods, in improving physiological traits, growth, yield, and seed Zn biofortification in sunflower (Helianthus annuus L.) under calcareous soil conditions”

✓ located verbatim in the source · abstract, characters 144–429 · show in text ↑
e3

“Foliar application of [Zn(Met)₂] was the most effective treatment, increasing seed yield by approximately 85%, oil yield by about 2.5-fold, and seed Zn uptake by nearly twofold, while also enhancing physiological traits, antioxidant enzyme activities, and protein accumulation”

✓ located verbatim in the source · abstract, characters 969–1245 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

The N/S ratio decreased concomitantly with increased seed oil content.

Taxon
sunflowerHelianthus annuuse1
Organ / stage
seede4
Outcome
N/S ratioe4
Direction
decreasede4
Qualifier
concomitantly with increased seed oil contente4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e1

“This study assessed the effectiveness of Zn-aminochelates, compared with conventional ZnSO₄, applied through various application methods, in improving physiological traits, growth, yield, and seed Zn biofortification in sunflower (Helianthus annuus L.) under calcareous soil conditions”

✓ located verbatim in the source · abstract, characters 144–429 · show in text ↑
e4

“The N/S ratio decreased concomitantly with increased seed oil content”

✓ located verbatim in the source · abstract, characters 1247–1316 · show in text ↑
Mechanismauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The superior performance of methionine-based Zn chelates is likely linked to their role in sulfur metabolism, Zn mobility and antioxidant regulation.

Taxon
sunflowerHelianthus annuuse1
Treatment
methionine-based Zn chelatese5
Outcome
superior performancee5
Qualifier
role in sulfur metabolism, enhanced Zn mobility, and improved antioxidant regulatione5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e1

“This study assessed the effectiveness of Zn-aminochelates, compared with conventional ZnSO₄, applied through various application methods, in improving physiological traits, growth, yield, and seed Zn biofortification in sunflower (Helianthus annuus L.) under calcareous soil conditions”

✓ located verbatim in the source · abstract, characters 144–429 · show in text ↑
e5

“The superior performance of methionine-based Zn chelates is likely linked to their role in sulfur metabolism, enhanced Zn mobility, and improved antioxidant regulation”

✓ located verbatim in the source · abstract, characters 1331–1498 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Foliar application of Zn-aminochelates is an effective and practical biofortification strategy for sunflower productivity and nutritional quality on calcareous soils.

Taxon
sunflowerHelianthus annuuse6
Treatment
foliar application of Zn-aminochelatese6
Outcome
productivity and nutritional qualitye6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e6

“Overall, foliar application of Zn-aminochelates represents an effective and practical agronomic biofortification strategy for improving productivity and nutritional quality of sunflower grown on calcareous soils”

✓ located verbatim in the source · abstract, characters 1500–1711 · show in text ↑

zinc atom · nutrient: content · Helianthus annuus (common sunflower)Results differ

13 papers: 8 positive, 5 negative or no effect

7 papers in the same direction as this paper · 5 in the opposite direction

  1. no effect
  2. no effect
  3. negative

9 papers more in this group

See all 13 papers →

zinc atom · quality: oil · Helianthus annuus (common sunflower)Results differ

17 papers: 14 positive, 3 negative or no effect

13 papers in the same direction as this paper · 3 in the opposite direction

  1. negative
  2. no effect
  3. no effect

13 papers more in this group

See all 17 papers →

zinc atom · yield · Helianthus annuus (common sunflower)Results differ

46 papers: 43 positive, 3 negative or no effect

42 papers in the same direction as this paper · 3 in the opposite direction

  1. negative
  2. negative
  3. no effect

42 papers more in this group

See all 46 papers →

application · nutrient: content · Helianthus annuus (common sunflower)Positive and negative results

25 papers: 24 positive, 1 negative or no effect

23 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

21 papers more in this group

See all 25 papers →

application · quality: oil · Helianthus annuus (common sunflower)Positive and negative results

45 papers: 41 positive, 4 negative or no effect

40 papers in the same direction as this paper · 3 in the opposite direction · 1 reporting no difference from an alternative

  1. no effect
  2. no effect
  3. no effect

41 papers more in this group

See all 45 papers →

application · yield · Helianthus annuus (common sunflower)Positive and negative results

158 papers: 152 positive, 6 negative or no effect

151 papers in the same direction as this paper · 5 in the opposite direction · 1 reporting no difference from an alternative

  1. no effect
  2. no effect
  3. negative

154 papers more in this group

See all 158 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.