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“dry weight decrease”

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Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

High runs of homozygosity were associated with decreased dry weight.

Gene: ROHse0
Outcome: dry weighte0
Direction: decreasee0
Qualifier: high ROHse0

Not reportedTaxon

Verbatim

e0
“The ROHs were associated with total weight and dry weight, and high ROHs resulted in a decrease in the two traits.”
✓ located verbatim in the source · abstract, characters 1363–1477

Title

Genome‐wide association study of multiple yield traits in a diversity panel of polyploid sugarcane ( Saccharum spp.)

Xiping Yang; Ziliang Luo; James Todd; et al. The Plant Genome 2020. doi:10.1002/tpg2.20006

Other claims from this paper (7)

Method / resource The diversity panel contained many sequence variants identified by target enrichment sequencing.
Association The study identified 217 nonredundant markers significantly associated with yield traits.
Association The study identified 225 candidate genes significantly associated with yield traits.
Observation The panel had 282 characterized runs of homozygosity.
Association Runs of homozygosity occurred nonrandomly and were probably under selection.
Association High runs of homozygosity were associated with decreased total weight.
Mechanism Genomic inbreeding was interpreted to harm sugarcane yield.

Mechanismauthor interpretationoriginal resultnegativemeasured

Claim · model-written summary

Sugar and organic nitrogen changes decreased the fresh to dry weight ratio in OsDOF11 mutants.

Taxon: RiceOryza sativat0
Gene: OsDOF11e0
Outcome: ratio of fresh weight to dry weighte0
Direction: decreasee0
Qualifier: sugar and organic nitrogen changese0

Verbatim

e0
“Both sugar and organic nitrogen changes caused the ratio of fresh weight to dry weight to decrease in OsDOF11 mutant seedling plants and mature leaves”
✓ located verbatim in the source · abstract, characters 985–1135

t0
“Rice (Oryza sativa L.)”
✓ located verbatim in the source · title, characters 70–92

Title

OsDOF11 Affects Nitrogen Metabolism by Sucrose Transport Signaling in Rice (Oryza sativa L.)

Xinglei Huang; Yiyan Zhang; Leilei Wang; et al. Frontiers in Plant Science 2021. doi:10.3389/fpls.2021.703034

Other claims from this paper (7)

Mechanism OsDOF11 promoted nitrogen uptake in seedlings and flowering leaf blades.
Mechanism OsDOF11 maintained the fresh to dry weight ratio.
Observation OsSWEET14 mutants had a phenotype similar to OsDOF11 mutants.
Mechanism OsDOF11 affected transcription of amino acid metabolism genes.
Observation Amino acid contents were reduced in flag leaves.
Observation Amino acid contents increased in seeds.
Mechanism The authors suggested reduced metabolic activity and stress susceptibility in OsDOF11 mutants.

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Double mutants had 35.2% lower total seed RFO content.

Taxon: soybeanGlycine maxt0
Outcome: total RFO contente0
Direction: decreasee0
Value: 35.2%e0
Qualifier: 64.7 mg/g dry weight to 41.95 mg/g dry weighte0

Verbatim

e0
“reduction in the total RFO content of soybean seed from 64.7 mg/g dry weight to 41.95 mg/g dry weight, a 35.2% decrease”
✓ located verbatim in the source · abstract, characters 875–994

t0
“Soybean Seeds”
✓ located verbatim in the source · title, characters 119–132

Title

CRISPR/Cas9-Mediated Knockout of Galactinol Synthase-Encoding Genes Reduces Raffinose Family Oligosaccharide Levels in Soybean Seeds

Huy Le; Nhung Hong Nguyen; Dong Thị Ta; et al. Frontiers in Plant Science 2020. doi:10.3389/fpls.2020.612942

Other claims from this paper (8)

Observation The construct induced deletions at both GOLS targets.
Observation Single and double mutant null segregants were identified without off-target induced mutations.
Observation Stachyose content decreased 35.4% in double mutants.
Observation Raffinose content increased 41.7% in double mutants.
Observation Verbascose content decreased slightly in mutant lines.
Observation Some mutant lines had increased protein and fat contents.
Observation Mutants showed no difference in seed weight, germination, development, or morphology.
Mechanism GmGOLS1A and GmGOLS1B contribute to the soybean oligosaccharide profile.

Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

High runs of homozygosity were associated with decreased total weight.

Gene: ROHse0
Outcome: total weighte0
Direction: decreasee0
Qualifier: high ROHse0

Not reportedTaxon

Verbatim

e0
“The ROHs were associated with total weight and dry weight, and high ROHs resulted in a decrease in the two traits.”
✓ located verbatim in the source · abstract, characters 1363–1477

Title

Genome‐wide association study of multiple yield traits in a diversity panel of polyploid sugarcane ( Saccharum spp.)

Xiping Yang; Ziliang Luo; James Todd; et al. The Plant Genome 2020. doi:10.1002/tpg2.20006

Other claims from this paper (7)

Method / resource The diversity panel contained many sequence variants identified by target enrichment sequencing.
Association The study identified 217 nonredundant markers significantly associated with yield traits.
Association The study identified 225 candidate genes significantly associated with yield traits.
Observation The panel had 282 characterized runs of homozygosity.
Association Runs of homozygosity occurred nonrandomly and were probably under selection.
Association High runs of homozygosity were associated with decreased dry weight.
Mechanism Genomic inbreeding was interpreted to harm sugarcane yield.

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Double mutant seeds had 35.2% less total RFO content, falling from 64.7 to 41.95 mg/g dry weight.

Taxon: soybeanGlycine maxe0
Material: double mutant linese0
Organ / stage: seede0
Treatment: double mutant linese0
Outcome: total RFO contente0
Direction: reductione0
Value: 35.2%e0

Not reportedComparator

Verbatim

e0
“The seed carbohydrate analysis of double mutant lines showed a reduction in the total RFO content of soybean seed from 64.7 mg/g dry weight to 41.95 mg/g dry weight, a 35.2% decrease”
✓ located verbatim in the source · abstract, characters 804–986

Title

CRISPR/Cas9-mediated knockout of galactinol synthase-encoding genes reduces raffinose family oligosaccharide levels in soybean seeds

Phat Do; Ha Chu; Huy Le; et al. Proceedings of The 1st International Electronic Conference on Plant Science 2020. doi:10.3390/iecps2020-08852

Other claims from this paper (9)

Method / resource The CRISPR construct induced deletions at target sites of both genes.
Observation Some induced alleles passed to progeny and T2 null segregants lacked induced off-target mutations.
Intervention effect Stachyose content declined by 35.4% in double mutant soybean.
Intervention effect Raffinose content rose by 41.7% in double mutant soybean.
Intervention effect Verbascose content decreased slightly in mutant lines.
Intervention effect Some mutant lines had increased seed protein and fat contents.
Intervention effect Mutants showed no observed differences in seed germination, development or morphology.
Mechanism The authors infer that GmGOLS1A and GmGOLS1B contribute to soybean oligosaccharide profiles through RFO biosynthesis.
Recommendation The authors present the genes as targets for crop improvement.

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