Search claims · PlantClaims

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The improved cacao somatic embryogenesis protocol gave better embryo recovery and survival than previous work

Taxon: Theobroma cacaoTheobroma cacaot0
Outcome: embryo recovery and survival ratee6
Comparator: previous worke6

Verbatim

t0
“Cacao (Theobroma cacao) Clones”
✓ located verbatim in the source · title, characters 38–68

e6
“describes an improved protocol compared with previous work in terms of embryo recovery and survival rate”
✓ located verbatim in the source · abstract, characters 1874–1978

Title

Enhanced Plantlet Regeneration in Two Cacao (Theobroma cacao) Clones from Immature Inflorescence Explants

Jane Kahia; Siaka Kone; Lucien Diby; et al. HortScience 2017. doi:10.21273/hortsci11844-17

Other claims from this paper (6)

Intervention effect 10 µM 2,4-D with 5 µM kinetin gave the highest mean embryo number per explant in cacao clone C1
Intervention effect 10 µM 2,4-D with 5 µM kinetin increased embryo number in cacao clone C1 9-fold relative to the control medium
Intervention effect 10 µM 2,4-D with 2.5 µM kinetin gave the highest mean embryo number per explant in cacao clone C14
Intervention effect 20 µM 2,4-D with 2.5 µM kinetin gave the highest mean embryo number in cacao clones C1 and C14
Observation Regenerated cacao embryos weaned in the greenhouse had a 70% survival rate
Observation Somatic embryogenesis of cacao clones is genotype dependent

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The protocol produced 20 transgenic clones per 0.5g inoculated tissue with 80% embryo survival in Kent mango.

Taxon: mangoMangifera indicat0
Material: Kente1
Outcome: transgenic clonese0
Value: Twenty transgenic clones per 0.5ge0
Qualifier: 80% embryo survivale0

Verbatim

e0
“Twenty transgenic clones per 0.5g of inoculated tissues with 80% embryo survival were produced”
✓ located verbatim in the source · abstract, characters 1338–1432

e1
“transgenic somatic embryos and plants of the Kent variety”
✓ located verbatim in the source · abstract, characters 1259–1316

t0
“mango”
✓ located verbatim in the source · title, characters 51–56

Title

Insertion of Agrobacterium rhizogenes rolB Gene in mango

Marleny Chavarri; Ariadne Vegas García; Asia Y. Zambrano; et al. Redalyc (Universidad Autónoma del Estado de México) 2010

Other claims from this paper (5)

Method / resourceobservedoriginal resultpositivenot significant (as stated)measured

Claim · model-written summary

Zygotic embryo survival after cryopreservation was 100%, without significant treatment differences.

Taxon: Jatropha curcas L.Jatropha curcast0
Treatment: cryopreservatione0
Outcome: Survival of zygotic embryose0
Value: 100%e0

Verbatim

e0
“Survival of zygotic embryos after cryopreservation was also 100%, without significant differences between treatments.”
✓ located verbatim in the source · abstract, characters 662–779

t0
“Jatropha curcas L.”
✓ located verbatim in the source · title, characters 41–59

Title

Cryopreservation of Seeds and Embryos of Jatropha curcas L.

Julián Andrés Prada; María Elena Aguilar; Ana Abdelnour-Esquivel; et al. American Journal of Plant Sciences 2015. doi:10.4236/ajps.2015.61020

Other claims from this paper (6)

Method / resource Seed germination after liquid nitrogen exposure was 100%.
Intervention effect Plantlet development was better in sand substrate than in vitro.
Method / resource Embryos dried for 60 min to 9.4% moisture before cryopreservation had optimal development of 100%.
Method / resource Embryos dried for 60 min to 9.4% moisture before cryopreservation had plantlet length of 51.77 mm.
Method / resource Vitrified zygotic embryos did not withstand liquid nitrogen exposure.
Method / resource Survival of non-cryopreserved embryos after vitrification steps provided information about cryoprotectant tolerance.

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Encapsulation dehydration gave the highest embryo survival rate, 85%.

Taxon: Paeonia lactiflora Pall.Paeonia lactiflorat0
Organ / stage: embryose0
Treatment: encapsulation-dehydratione0
Outcome: survival ratee0
Direction: higheste0
Value: 85%e0

Verbatim

e0
“The highest survival rate (85%) was obtained from embryos treated by encapsulation-dehydration.”
✓ located verbatim in the source · abstract, characters 180–275

t0
“Paeonia lactiflora Pall.”
✓ located verbatim in the source · title, characters 56–80

Title

Cryopreservation of Zygotic Embryos of Herbaceous Peony(Paeonia lactiflora Pall.) by Encapsulation-Dehydration

Hyun-Mi Kim; Jong-Hee Shin; Jea-Keun Sohn The Korean Journal of Crop Science 2004

Other claims from this paper (1)

Recommendation The authors considered encapsulation dehydration promising for peony germplasm cryopreservation.

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Sweet orange embryo axes encapsulated in Na-alginate beads, precultured with high sucrose and dehydrated over silica gel before freezing in liquid nitrogen had 60% survival.

Taxon: Sweet OrangeCitrus sinensist0
Organ / stage: Embryo axese1
Outcome: survivale1
Value: 60%e1

Verbatim

e1
“Embryo axes encapsualted in Na-alginate beads, precultured with high levels of sucrose and dehydrated over silica gel before freezing in liquid nitrogen had 60% survival”
✓ located verbatim in the source · abstract, characters 821–990

t0
“Sweet Orange”
✓ located verbatim in the source · title, characters 68–80

Title

Cryopreservation of Embryonic Axes and Axillary Buds of `Pineapple' Sweet Orange [Citrus sinensis (L.) Osb.] Encapsulated in Alginate Gel

Izulme R. Santos; Cecil Stushnoff HortScience 1997. doi:10.21273/hortsci.32.3.440d

Other claims from this paper (2)

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Phase diagrams predicted the observed embryo survival trends.

Taxon: ZizaniaZizaniat0
Outcome: trends were predictede0
Comparator: These trendse0

Verbatim

e0
“These trends were predicted by ‘phase diagrams’ based on the physical properties of water in embryos at different stages of maturity”
✓ located verbatim in the source · abstract, characters 1061–1193

t0
“Survival of Zizania embryos in relation to water content, temperature and maturity status”
✓ located verbatim in the source · title, characters 0–89

Title

Survival of Zizania embryos in relation to water content, temperature and maturity status

Christina W. Vertucci; Jennifer Crane; Raymond A. Porter; et al. Seed Science Research 1995. doi:10.1017/s0960258500002567

Other claims from this paper (7)

Association Embryo viability declined below a critical water content.
Association The critical water content was greatest in the least mature embryos.
Association Critical water content increased as temperature decreased.
Observation Water activity at the critical water content appeared constant at 0.90 despite developmental and temperature variation.
Observation The most mature embryos survived temperatures down to −50°C.
Observation The least mature embryos survived only to −18°C.
Recommendation Long-term preservation of Zizania grains is possible at −20°C, with success related to embryo maturity.

Method / resourceobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

After 20 years of cryostorage, mean somatic embryo survival was significantly higher at 33.2% among 28 surviving clones.

Taxon: Oil Palmt0
Outcome: survival of SEse0
Direction: highere0
Value: 33.2%e0
Comparator: 20 years of cryostoragee0

Verbatim

e0
“survival of SEs rewarmed after 20 years of cryostorage was significantly higher, with an average of 33.2% for the 28 surviving clones”
✓ located verbatim in the source · abstract, characters 773–906

t0
“Oil Palm (Elaeis guineensis Jacq.)”
✓ located verbatim in the source · title, characters 12–46

Title

Recovery of Oil Palm (Elaeis guineensis Jacq.) Somatic Embryos Cryostored For 20 Years.

T Beule; P Ilbert; K Adeoti; et al. PubMed 2018

Other claims from this paper (6)

Method / resource Immediately rewarmed cryopreserved somatic embryos had mean survival of 19.1% across 29 clones.
Observation Three of the 28 surviving clones were lost to contamination or regrowth decline.
Observation Six surviving clones produced only shoots.
Observation The remaining surviving clones proliferated.
Observation Extended cryostorage can regenerate proliferating cultures and plantlets from oil palm somatic embryos.
Recommendation The authors conclude the protocol can be used to store oil palm germplasm and manage large-scale industrial production.

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The nucellar embryo transformation procedure generated modified black pepper with 99% survival.

Taxon: black peppere0
Material: Sreekara and Karimundae0
Organ / stage: nucellar apomixis-derived embryose0
Outcome: survival ratee0
Value: 99%e0

Verbatim

e0
“generating genetically modified black pepper plants using nucellar apomixis-derived embryos of the varieties Sreekara and Karimunda, with a 99% survival rate”
✓ located verbatim in the source · abstract, characters 423–580

Title

Reducing recalcitrance of black pepper to Agrobacterium -mediated transformation: an efficient way through nucellar apomixis to establish transgenic and genome-edited plants at high frequency and scale-up through bioreactor

Shina Sasi; Saranya Krishnan; Martin Kottackal; et al. Horticulture Research 2026. doi:10.1093/hr/uhag067

Other claims from this paper (11)

Method / resource Agrobacterium tumefaciens transformed black pepper efficiently.
Method / resource Agrobacterium rhizogenes transformed black pepper efficiently.
Method / resource AGL1 carrying mgfp achieved over 90% transformation frequency under the stated procedure.
Association Sugar type determined embryonal taproot development and soil establishment.
Intervention effect Glucose supplemented medium yielded plantlets with developed roots.
Observation Plantlets from glucose supplemented medium had high PnPIN2 expression.
Method / resource Transgenic plantlets from glucose supplemented liquid medium survived ex vitro at 99%.
Method / resource CRISPR/Cas9 editing of Pds reached 89% efficiency.
Method / resource Composite black pepper plants were generated at over 60% efficacy.
Method / resource Black pepper agroinfiltration was useful for disease resistance screening.
Method / resource Temporary immersion bioreactors scaled production of modified and unmodified black pepper plants.

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Encapsulation-dehydration produced 37.50% embryo survival.

Taxon: Hevea brasiliensisHevea brasiliensist0
Organ / stage: encapsulated zygotic embryose0
Treatment: encapsulation-dehydration methode0
Outcome: survival ratee0
Value: 37.50%e0

Verbatim

t0
“Hevea brasiliensis”
✓ located verbatim in the source · title, characters 20–38

e0
“Cryopreservation by the encapsulation-dehydration method was successfully done by leaving the encapsulated zygotic embryos in a laminar flow for 4 hours prior to plunging into a LN. The survival rate and regrowth of the encapsulated zygotic embryos were 37.50% and 27.98%, respectively”
✓ located verbatim in the source · abstract, characters 734–1019

Title

Cryopreservation of Hevea brasiliensis zygotic embryos by vitrification and encapsulation-dehydration

Korakot Nakkanong; Charassri Nualsri Journal of Plant Biotechnology 2018. doi:10.5010/jpb.2018.45.4.333

Other claims from this paper (4)

Method / resource Vitrification with loading solution and PVS2 gave the highest embryo survival rate, 88.87%.
Method / resource The PVS2 vitrification procedure produced 66.33% embryo regrowth.
Method / resource Encapsulation-dehydration produced 27.98% embryo regrowth.
Observation Cryopreserved Hevea embryos developed into whole plants.

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Survival of non-cryopreserved embryos after vitrification steps provided information about cryoprotectant tolerance.

Taxon: Jatropha curcas L.Jatropha curcast0
Outcome: embryo tolerance to cryoprotectantse0
Comparator: non-cryopreserved embryose0

Verbatim

e0
“Survival data for non-cryopreserved embryos after each step of the vitrification procedure provided information about embryo tolerance to cryoprotectants.”
✓ located verbatim in the source · abstract, characters 1027–1181

t0
“Jatropha curcas L.”
✓ located verbatim in the source · title, characters 41–59

Title

Cryopreservation of Seeds and Embryos of Jatropha curcas L.

Julián Andrés Prada; María Elena Aguilar; Ana Abdelnour-Esquivel; et al. American Journal of Plant Sciences 2015. doi:10.4236/ajps.2015.61020

Other claims from this paper (6)

Method / resource Seed germination after liquid nitrogen exposure was 100%.
Intervention effect Plantlet development was better in sand substrate than in vitro.
Method / resource Zygotic embryo survival after cryopreservation was 100%, without significant treatment differences.
Method / resource Embryos dried for 60 min to 9.4% moisture before cryopreservation had optimal development of 100%.
Method / resource Embryos dried for 60 min to 9.4% moisture before cryopreservation had plantlet length of 51.77 mm.
Method / resource Vitrified zygotic embryos did not withstand liquid nitrogen exposure.

“embryo survival”

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