By organism › Green plants › Fabaceae › Lotus japonicus

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

PACE was essential for restoring infection threads in nin mutants of Lotus japonicus.

Taxon: Lotus japonicusLotus japonicuse1
Material: nin mutantse1
Treatment: PACEe1
Gene: NINe0
Outcome: infection threadse1
Direction: restoringe1

Not reportedComparator

Verbatim

e0
“Here we discovered-within the promoter of the transcription factor gene Nodule Inception (NIN)-a cis-regulatory element (PACE), exclusively present in members of this clade”
✓ located verbatim in the source · abstract, characters 240–412

e1
“PACE was essential for restoring infection threads in nin mutants of the legume Lotus japonicus”
✓ located verbatim in the source · abstract, characters 414–509

Title

A novel cis-element enabled bacterial uptake by plant cells

Chloé Cathebras; Xiaoyun Gong; Rosa Elena Andrade; et al. Nature Plants 2026. doi:10.1038/s41477-025-02161-z

Other claims from this paper (5)

Observationobservedoriginal resultmeasured

Claim · model-written summary

During early nodule development, PACE dictates gene expression in a spatially restricted domain of cortical cells carrying infection threads in Lotus japonicus.

Taxon: Lotus japonicusLotus japonicuse1
Organ / stage: cortical cellse3
Gene: PACEe3
Outcome: gene expressione3
Qualifier: spatially restricted domaine3

Verbatim

e1
“PACE was essential for restoring infection threads in nin mutants of the legume Lotus japonicus”
✓ located verbatim in the source · abstract, characters 414–509

e3
“During the early stages of nodule development, PACE dictates gene expression in a spatially restricted domain containing cortical cells carrying infection threads”
✓ located verbatim in the source · abstract, characters 694–856

Title

A novel cis-element enabled bacterial uptake by plant cells

Chloé Cathebras; Xiaoyun Gong; Rosa Elena Andrade; et al. Nature Plants 2026. doi:10.1038/s41477-025-02161-z

Other claims from this paper (5)

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ljasn1 mutants grown under NS conditions produced much higher biomass than WT.

Taxon: Lotus japonicusLotus japonicust0
Material: Ljasn1 mutantse1
Treatment: NS conditionse1
Outcome: biomasse1
Direction: highere1
Comparator: WTe1

Verbatim

t0
“Lotus japonicus”
✓ located verbatim in the source · title, characters 74–89

e1
“a much higher biomass was produced in Ljasn1 mutants grown under NS conditions compared to the WT (wild-type)”
✓ located verbatim in the source · abstract, characters 463–572

Title

Disruption of Asparagine Synthetase Is Associated to Increased Biomass in Lotus japonicus

Sara Rosa‐Téllez; Margarita García‐Calderón; David Barbosa Medeiros; et al. Plant Biotechnology Journal 2026. doi:10.1111/pbi.70637

Other claims from this paper (9)

Intervention effect Ljasn1 mutant biomass showed little difference from WT under S conditions.
Observation Metabolomic profiles of Ljasn1 mutants were quite distinct from WT under NS conditions but not under S conditions.
Intervention effect Asn levels were considerably reduced in Ljasn1 mutants compared with WT under NS conditions.
Intervention effect Asn levels did not differ between Ljasn1 mutants and WT under S conditions.
Intervention effect Amino acids decreased in NS roots of Ljasn1 mutants.
Intervention effect Carbon compounds such as sugars and oxo-acids increased in NS shoots.
Observation RNAseq showed changes related to oxidative metabolism under NS conditions.
Observation RNAseq showed changes related to C/N metabolism under S conditions.
Mechanism LjASN1 deficiency produces changes in the C/N balance and metabolite allocation of L. japonicus.

Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Ljasn1 mutant biomass showed little difference from WT under S conditions.

Taxon: Lotus japonicusLotus japonicust0
Material: mutant plantse1
Treatment: S conditionse1
Outcome: biomasse1
Direction: no_changeinferrede1
Comparator: WTe1

Verbatim

t0
“Lotus japonicus”
✓ located verbatim in the source · title, characters 74–89

e1
“a much higher biomass was produced in Ljasn1 mutants grown under NS conditions compared to the WT (wild-type), whereas little difference with the WT was observed in mutant plants under S conditions”
✓ located verbatim in the source · abstract, characters 463–660

Title

Disruption of Asparagine Synthetase Is Associated to Increased Biomass in Lotus japonicus

Sara Rosa‐Téllez; Margarita García‐Calderón; David Barbosa Medeiros; et al. Plant Biotechnology Journal 2026. doi:10.1111/pbi.70637

Other claims from this paper (9)

Intervention effect Ljasn1 mutants grown under NS conditions produced much higher biomass than WT.
Observation Metabolomic profiles of Ljasn1 mutants were quite distinct from WT under NS conditions but not under S conditions.
Intervention effect Asn levels were considerably reduced in Ljasn1 mutants compared with WT under NS conditions.
Intervention effect Asn levels did not differ between Ljasn1 mutants and WT under S conditions.
Intervention effect Amino acids decreased in NS roots of Ljasn1 mutants.
Intervention effect Carbon compounds such as sugars and oxo-acids increased in NS shoots.
Observation RNAseq showed changes related to oxidative metabolism under NS conditions.
Observation RNAseq showed changes related to C/N metabolism under S conditions.
Mechanism LjASN1 deficiency produces changes in the C/N balance and metabolite allocation of L. japonicus.

Observationobservedoriginal resultmeasured

Claim · model-written summary

Metabolomic profiles of Ljasn1 mutants were quite distinct from WT under NS conditions but not under S conditions.

Taxon: Lotus japonicusLotus japonicust0
Material: Ljasn1 mutant plantse1
Treatment: NS conditionse1
Outcome: Metabolomic analysise1

Verbatim

t0
“Lotus japonicus”
✓ located verbatim in the source · title, characters 74–89

e1
“Metabolomic analysis revealed that Ljasn1 mutant plants are quite distinct to WT plants when grown under NS conditions, but not under S conditions”
✓ located verbatim in the source · abstract, characters 662–808

Title

Disruption of Asparagine Synthetase Is Associated to Increased Biomass in Lotus japonicus

Sara Rosa‐Téllez; Margarita García‐Calderón; David Barbosa Medeiros; et al. Plant Biotechnology Journal 2026. doi:10.1111/pbi.70637

Other claims from this paper (9)

Intervention effect Ljasn1 mutants grown under NS conditions produced much higher biomass than WT.
Intervention effect Ljasn1 mutant biomass showed little difference from WT under S conditions.
Intervention effect Asn levels were considerably reduced in Ljasn1 mutants compared with WT under NS conditions.
Intervention effect Asn levels did not differ between Ljasn1 mutants and WT under S conditions.
Intervention effect Amino acids decreased in NS roots of Ljasn1 mutants.
Intervention effect Carbon compounds such as sugars and oxo-acids increased in NS shoots.
Observation RNAseq showed changes related to oxidative metabolism under NS conditions.
Observation RNAseq showed changes related to C/N metabolism under S conditions.
Mechanism LjASN1 deficiency produces changes in the C/N balance and metabolite allocation of L. japonicus.

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Asn levels were considerably reduced in Ljasn1 mutants compared with WT under NS conditions.

Taxon: Lotus japonicusLotus japonicust0
Material: Ljasn1 mutant plantse1
Treatment: NSe1
Outcome: Asn levelse1
Direction: reducede1
Comparator: WTe1

Verbatim

t0
“Lotus japonicus”
✓ located verbatim in the source · title, characters 74–89

e1
“Asn levels were considerably reduced in Ljasn1 mutant plants compared to the WT when plants were grown under NS but not under S conditions”
✓ located verbatim in the source · abstract, characters 810–948

Title

Disruption of Asparagine Synthetase Is Associated to Increased Biomass in Lotus japonicus

Sara Rosa‐Téllez; Margarita García‐Calderón; David Barbosa Medeiros; et al. Plant Biotechnology Journal 2026. doi:10.1111/pbi.70637

Other claims from this paper (9)

Intervention effect Ljasn1 mutants grown under NS conditions produced much higher biomass than WT.
Intervention effect Ljasn1 mutant biomass showed little difference from WT under S conditions.
Observation Metabolomic profiles of Ljasn1 mutants were quite distinct from WT under NS conditions but not under S conditions.
Intervention effect Asn levels did not differ between Ljasn1 mutants and WT under S conditions.
Intervention effect Amino acids decreased in NS roots of Ljasn1 mutants.
Intervention effect Carbon compounds such as sugars and oxo-acids increased in NS shoots.
Observation RNAseq showed changes related to oxidative metabolism under NS conditions.
Observation RNAseq showed changes related to C/N metabolism under S conditions.
Mechanism LjASN1 deficiency produces changes in the C/N balance and metabolite allocation of L. japonicus.

Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Asn levels did not differ between Ljasn1 mutants and WT under S conditions.

Taxon: Lotus japonicusLotus japonicust0
Material: Ljasn1 mutant plantse1
Treatment: S conditionse1
Outcome: Asn levelse1
Direction: no_changeinferrede1
Comparator: WTe1

Verbatim

t0
“Lotus japonicus”
✓ located verbatim in the source · title, characters 74–89

e1
“Asn levels were considerably reduced in Ljasn1 mutant plants compared to the WT when plants were grown under NS but not under S conditions”
✓ located verbatim in the source · abstract, characters 810–948

Title

Disruption of Asparagine Synthetase Is Associated to Increased Biomass in Lotus japonicus

Sara Rosa‐Téllez; Margarita García‐Calderón; David Barbosa Medeiros; et al. Plant Biotechnology Journal 2026. doi:10.1111/pbi.70637

Other claims from this paper (9)

Intervention effect Ljasn1 mutants grown under NS conditions produced much higher biomass than WT.
Intervention effect Ljasn1 mutant biomass showed little difference from WT under S conditions.
Observation Metabolomic profiles of Ljasn1 mutants were quite distinct from WT under NS conditions but not under S conditions.
Intervention effect Asn levels were considerably reduced in Ljasn1 mutants compared with WT under NS conditions.
Intervention effect Amino acids decreased in NS roots of Ljasn1 mutants.
Intervention effect Carbon compounds such as sugars and oxo-acids increased in NS shoots.
Observation RNAseq showed changes related to oxidative metabolism under NS conditions.
Observation RNAseq showed changes related to C/N metabolism under S conditions.
Mechanism LjASN1 deficiency produces changes in the C/N balance and metabolite allocation of L. japonicus.

Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Amino acids decreased in NS roots of Ljasn1 mutants.

Taxon: Lotus japonicusLotus japonicust0
Organ / stage: rootse1
Treatment: NSe1
Outcome: amino acidse1
Direction: decreasee1

Not reportedComparator

Verbatim

t0
“Lotus japonicus”
✓ located verbatim in the source · title, characters 74–89

e1
“A general decrease in amino acids and an increase in carbon compounds, such as sugars and oxo-acids, was detected in NS roots and shoots, respectively”
✓ located verbatim in the source · abstract, characters 950–1100

Title

Disruption of Asparagine Synthetase Is Associated to Increased Biomass in Lotus japonicus

Sara Rosa‐Téllez; Margarita García‐Calderón; David Barbosa Medeiros; et al. Plant Biotechnology Journal 2026. doi:10.1111/pbi.70637

Other claims from this paper (9)

Intervention effect Ljasn1 mutants grown under NS conditions produced much higher biomass than WT.
Intervention effect Ljasn1 mutant biomass showed little difference from WT under S conditions.
Observation Metabolomic profiles of Ljasn1 mutants were quite distinct from WT under NS conditions but not under S conditions.
Intervention effect Asn levels were considerably reduced in Ljasn1 mutants compared with WT under NS conditions.
Intervention effect Asn levels did not differ between Ljasn1 mutants and WT under S conditions.
Intervention effect Carbon compounds such as sugars and oxo-acids increased in NS shoots.
Observation RNAseq showed changes related to oxidative metabolism under NS conditions.
Observation RNAseq showed changes related to C/N metabolism under S conditions.
Mechanism LjASN1 deficiency produces changes in the C/N balance and metabolite allocation of L. japonicus.

Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Carbon compounds such as sugars and oxo-acids increased in NS shoots.

Taxon: Lotus japonicusLotus japonicust0
Organ / stage: shootse1
Treatment: NSe1
Outcome: carbon compoundse1
Direction: increasee1

Not reportedComparator

Verbatim

t0
“Lotus japonicus”
✓ located verbatim in the source · title, characters 74–89

e1
“A general decrease in amino acids and an increase in carbon compounds, such as sugars and oxo-acids, was detected in NS roots and shoots, respectively”
✓ located verbatim in the source · abstract, characters 950–1100

Title

Disruption of Asparagine Synthetase Is Associated to Increased Biomass in Lotus japonicus

Sara Rosa‐Téllez; Margarita García‐Calderón; David Barbosa Medeiros; et al. Plant Biotechnology Journal 2026. doi:10.1111/pbi.70637

Other claims from this paper (9)

Intervention effect Ljasn1 mutants grown under NS conditions produced much higher biomass than WT.
Intervention effect Ljasn1 mutant biomass showed little difference from WT under S conditions.
Observation Metabolomic profiles of Ljasn1 mutants were quite distinct from WT under NS conditions but not under S conditions.
Intervention effect Asn levels were considerably reduced in Ljasn1 mutants compared with WT under NS conditions.
Intervention effect Asn levels did not differ between Ljasn1 mutants and WT under S conditions.
Intervention effect Amino acids decreased in NS roots of Ljasn1 mutants.
Observation RNAseq showed changes related to oxidative metabolism under NS conditions.
Observation RNAseq showed changes related to C/N metabolism under S conditions.
Mechanism LjASN1 deficiency produces changes in the C/N balance and metabolite allocation of L. japonicus.

Observationobservedoriginal resultmeasured

Claim · model-written summary

RNAseq showed changes related to oxidative metabolism under NS conditions.

Taxon: Lotus japonicusLotus japonicust0
Treatment: NS conditionse1
Outcome: changes related to oxidative metabolisme1

Verbatim

t0
“Lotus japonicus”
✓ located verbatim in the source · title, characters 74–89

e1
“RNAseq analysis showed changes related to oxidative metabolism under NS conditions”
✓ located verbatim in the source · abstract, characters 1152–1234

Title

Disruption of Asparagine Synthetase Is Associated to Increased Biomass in Lotus japonicus

Sara Rosa‐Téllez; Margarita García‐Calderón; David Barbosa Medeiros; et al. Plant Biotechnology Journal 2026. doi:10.1111/pbi.70637

Other claims from this paper (9)

Intervention effect Ljasn1 mutants grown under NS conditions produced much higher biomass than WT.
Intervention effect Ljasn1 mutant biomass showed little difference from WT under S conditions.
Observation Metabolomic profiles of Ljasn1 mutants were quite distinct from WT under NS conditions but not under S conditions.
Intervention effect Asn levels were considerably reduced in Ljasn1 mutants compared with WT under NS conditions.
Intervention effect Asn levels did not differ between Ljasn1 mutants and WT under S conditions.
Intervention effect Amino acids decreased in NS roots of Ljasn1 mutants.
Intervention effect Carbon compounds such as sugars and oxo-acids increased in NS shoots.
Observation RNAseq showed changes related to C/N metabolism under S conditions.
Mechanism LjASN1 deficiency produces changes in the C/N balance and metabolite allocation of L. japonicus.

Lotus japonicus 4,666 claims

4,666 claims