Abstract
Mature Nicotiana benthamiana shows strong resistance to the potato late blight pathogen Phytophthora infestans. By screening using virus-induced random gene silencing, we isolated a gene for plant-specific calreticulin NbCRT3a as a required gene for resistance of N. benthamiana against P. infestans. NbCRT3a encodes an endoplasmic reticulum quality-control (ERQC) chaperone for the maturation of glycoproteins, including glycosylated cell-surface receptors. NbCRT3a-silenced plants showed no detectable growth defects but resistance to P. infestans was significantly compromised. Defense responses induced by the treatment with INF1 (a secretory protein of P. infestans), such as production of reactive oxygen species and accumulation of phytoalexins, were suppressed in NbCRT3a-silenced N. benthamiana. Expression of an ethylene-regulated gene for phytoalexin biosynthesis, NbEAS, was reduced in NbCRT3a-silenced plants, whereas the expression of salicylic acid-regulated NbPR-1a was not affected. Consistently, induction of ethylene production by INF1 was suppressed in NbCRT3a-silenced plants. Resistance reactions induced by a hyphal wall components elicitor prepared from P. infestans were also impaired in NbCRT3a-silenced plants. However, cell death induced by active mitogen-activated protein kinase kinase (NbMEK2(DD)) was not affected by the silencing of NbCRT3a. Thus, NbCRT3a is required for the initiation of resistance reactions of N. benthamiana in response to elicitor molecules derived from P. infestans.
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Claims 13
Claim · model-written summary
Mature Nicotiana benthamiana strongly resisted P. infestans.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Interactor
- Phytophthora infestans
Phytophthora infestanse0pathogen - Outcome
- strong resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mature Nicotiana benthamiana shows strong resistance to the potato late blight pathogen Phytophthora infestans.”
Claim · model-written summary
The silencing screen identified NbCRT3a as required for resistance against P. infestans.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- NbCRT3ae0
- Outcome
- required gene for resistancee0
- Value
- required genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated a gene for plant-specific calreticulin NbCRT3a as a required gene for resistance of N. benthamiana against P. infestans.”
Claim · model-written summary
NbCRT3a encodes an ER quality-control chaperone for glycoprotein maturation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NbCRT3ae0
- Outcome
- maturation of glycoproteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbCRT3a encodes an endoplasmic reticulum quality-control (ERQC) chaperone for the maturation of glycoproteins, including glycosylated cell-surface receptors.”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbCRT3a silencing caused no detectable growth defects.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbCRT3a-silencede0
- Outcome
- growth defectse0
- Direction
- no detectablee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbCRT3a-silenced plants showed no detectable growth defects”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbCRT3a silencing significantly compromised resistance to P. infestans.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbCRT3a-silencede1
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Outcome
- resistance to P. infestanse0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“resistance to P. infestans was significantly compromised.”
e1“NbCRT3a-silenced plants showed no detectable growth defects but resistance”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbCRT3a silencing suppressed INF1-induced reactive oxygen species production.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- NbCRT3a-silencede0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Outcome
- production of reactive oxygen speciese0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Defense responses induced by the treatment with INF1 (a secretory protein of P. infestans), such as production of reactive oxygen species and accumulation of phytoalexins, were suppressed in NbCRT3a-silenced N. benthamiana.”
Claim · model-written summary
NbCRT3a silencing suppressed INF1-induced phytoalexin accumulation.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- NbCRT3a-silencede0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Outcome
- accumulation of phytoalexinse0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Defense responses induced by the treatment with INF1 (a secretory protein of P. infestans), such as production of reactive oxygen species and accumulation of phytoalexins, were suppressed in NbCRT3a-silenced N. benthamiana.”
Claim · model-written summary
NbCRT3a silencing reduced NbEAS expression.
- Taxon
- plantsViridiplantaee0
- Treatment
- NbCRT3a-silencede0
- Gene
- NbEASe0
- Outcome
- Expressione0
- Direction
- reducede0
Not reportedComparator
Verbatim
e0“Expression of an ethylene-regulated gene for phytoalexin biosynthesis, NbEAS, was reduced in NbCRT3a-silenced plants”
Claim · model-written summary
NbCRT3a silencing did not affect NbPR-1a expression.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbCRT3a-silencede1
- Gene
- NbPR-1ae0
- Outcome
- expressione0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of salicylic acid-regulated NbPR-1a was not affected.”
e1“NbEAS, was reduced in NbCRT3a-silenced plants”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbCRT3a silencing suppressed INF1-induced ethylene production.
- Taxon
- plantsViridiplantaee0
- Treatment
- NbCRT3a-silencede0
- Outcome
- ethylene productione0
- Direction
- suppressede0
Not reportedComparator
Verbatim
e0“induction of ethylene production by INF1 was suppressed in NbCRT3a-silenced plants.”
Claim · model-written summary
NbCRT3a silencing impaired resistance reactions induced by P. infestans wall components.
- Taxon
- plantsViridiplantaee0
- Treatment
- NbCRT3a-silencede0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Outcome
- Resistance reactionse0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Resistance reactions induced by a hyphal wall components elicitor prepared from P. infestans were also impaired in NbCRT3a-silenced plants.”
Claim · model-written summary
NbCRT3a silencing did not affect cell death induced by active NbMEK2(DD).
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- silencing of NbCRT3ae0
- Gene
- NbMEK2(DD)e0
- Outcome
- cell deathe0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cell death induced by active mitogen-activated protein kinase kinase (NbMEK2(DD)) was not affected by the silencing of NbCRT3a.”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbCRT3a was inferred to be necessary to initiate resistance to P. infestans elicitors.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- NbCRT3ae0
- Outcome
- initiation of resistance reactionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbCRT3a is required for the initiation of resistance reactions of N. benthamiana in response to elicitor molecules derived from P. infestans.”
The same questions in other papers
loss of function · cytology · Nicotiana benthamianaPositive and negative results
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See all 33 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.