Abstract
A zinc finger-homeodomain (ZFHD) gene was isolated from Triticum aestivum using in silico cloning and reverse transcription (RT-PCR) approaches. The full length cDNA of T. aestivum, designed as TaZFHD1 (GenBank accession No. KF697362), was 1345 bp long. The open reading frame (ORF) of TaZFHD1 was 1164 bp and was predicted to encode 387 amino-acid protein that contained putative zinc finger domain in the N-terminus and a DNA binding homeodomain in the C-terminus. Analysis of the deduced amino acid sequence of TaZFHD1 revealed that it had high homology to other plants ZFHD proteins. The expression of TaZFHD1 was analyzed during spike development and after exposure to different hormonal stimuli using semi-quantitative RT-PCR analysis. TaZFHD1 was differentially expressed during spike development, with a preferential expression during ‘half emerged’, ‘completely emerged’, and ‘half anthesis’ stages. Methyl jasmonate (MeJA), abcissic acid (ABA) and ethylene (ET) upregulated the expression of TaZFHD1. However, there was no remarkable difference in TaZFHD1 expression after treatment with salicylic acid (SA). These results might indicate that TaZFHD1 may be involved in wheat anthesis and pollination and in JA-, ABA-, and ET-mediated signaling pathways.
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Claims 9
Claim · model-written summary
TaZFHD1 cDNA was 1345 bp long.
- Taxon
- T. aestivum
Triticum aestivume0 - Gene
- TaZFHD1e0
- Outcome
- full length cDNAe0
- Value
- 1345 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The full length cDNA of T. aestivum, designed as TaZFHD1 (GenBank accession No. KF697362), was 1345 bp long.”
Claim · model-written summary
TaZFHD1 ORF was 1164 bp.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaZFHD1e0
- Outcome
- open reading frame (ORF)e0
- Value
- 1164 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“The open reading frame (ORF) of TaZFHD1 was 1164 bp”
Claim · model-written summary
TaZFHD1 was predicted to encode a 387 amino-acid protein with zinc finger and DNA binding homeodomains.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaZFHD1t0
- Outcome
- putative zinc finger domain in the N-terminus and a DNA binding homeodomain in the C-terminuse0
- Value
- 387 amino-acide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“TaZFHD1 in wheat”
e0“was predicted to encode 387 amino-acid protein that contained putative zinc finger domain in the N-terminus and a DNA binding homeodomain in the C-terminus”
Claim · model-written summary
TaZFHD1 had high homology to other plant ZFHD proteins.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaZFHD1e0
- Outcome
- high homology to other plants ZFHD proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“Analysis of the deduced amino acid sequence of TaZFHD1 revealed that it had high homology to other plants ZFHD proteins.”
Claim · model-written summary
TaZFHD1 was preferentially expressed during half emerged, completely emerged, and half anthesis spike stages.
- Taxon
- wheatTriticum aestivumt0
- Organ / stage
- spikee0
- Gene
- TaZFHD1e0
- Outcome
- preferential expressione0
- Qualifier
- ‘half emerged’, ‘completely emerged’, and ‘half anthesis’ stagese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“TaZFHD1 was differentially expressed during spike development, with a preferential expression during ‘half emerged’, ‘completely emerged’, and ‘half anthesis’ stages.”
Claim · model-written summary
Methyl jasmonate upregulated TaZFHD1 expression.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Methyl jasmonate (MeJA)e0
- Gene
- TaZFHD1e0
- Outcome
- expression of TaZFHD1e0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“Methyl jasmonate (MeJA), abcissic acid (ABA) and ethylene (ET) upregulated the expression of TaZFHD1.”
Claim · model-written summary
Abcissic acid upregulated TaZFHD1 expression.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- abcissic acid (ABA)e0
- Gene
- TaZFHD1e0
- Outcome
- expression of TaZFHD1e0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“Methyl jasmonate (MeJA), abcissic acid (ABA) and ethylene (ET) upregulated the expression of TaZFHD1.”
Claim · model-written summary
Ethylene upregulated TaZFHD1 expression.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- ethylene (ET)e0
- Gene
- TaZFHD1e0
- Outcome
- expression of TaZFHD1e0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“Methyl jasmonate (MeJA), abcissic acid (ABA) and ethylene (ET) upregulated the expression of TaZFHD1.”
Claim · model-written summary
Salicylic acid treatment showed no remarkable difference in TaZFHD1 expression.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- salicylic acid (SA)e0
- Gene
- TaZFHD1e0
- Outcome
- TaZFHD1 expressione0
- Direction
- no remarkable differencee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“there was no remarkable difference in TaZFHD1 expression after treatment with salicylic acid (SA).”
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See all 43 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.