Abstract
The response of soybean transgenic plants, with suppressed synthesis of isoflavones, and nontransgenic plants to two common soybean pathogens, Macrophomina phaseolina and Phytophthora sojae, was studied. Transgenic soybean plants of one line used in this study were previously generated via bombardment of embryogenic cultures with the phenylalanine ammonia lyase, chalcone synthase, and isoflavone synthase (IFS2) genes in sense orientation driven by the cotyledon-preferable lectin promoter (to turn genes on in cotyledons), while plants of another line were newly produced using the IFS2 gene in sense orientation driven by the Cassava vein mosaic virus constitutive promoter (to turn genes on in all plant parts). Nearly complete inhibition of isoflavone synthesis was found in the cotyledons of young seedlings of transgenic plants transformed with the IFS2 transgene driven by the cotyledon-preferable lectin promoter compared with the untransformed control during the 10-day observation period, with the precursors of isoflavone synthesis being accumulated in the cotyledons of transgenic plants. These results indicated that the lectin promoter could be active not only during seed development but also during seed germination. Downregulation of isoflavone synthesis only in the seed or in the whole soybean plant caused a strong inhibition of the pathogen-inducible glyceollin in cotyledons after inoculation with P. sojae, which resulted in increased susceptibility of the cotyledons of both transgenic lines to this pathogen compared with inoculated cotyledons of untransformed plants. When stems were inoculated with M. phaseolina, suppression of glyceollin synthesis was found only in stems of transgenic plants expressing the transgene driven by a constitutive promoter, which developed more severe infection. These results provide further evidence that rapid glyceollin accumulation during infection contributes to the innate soybean defense response.
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Claims 7
Claim · model-written summary
IFS2 transgenic cotyledons had nearly complete inhibition of isoflavone synthesis relative to controls.
- Taxon
- Soybean
Glycine maxt0 - Organ / stage
- cotyledons of young seedlingse0
- Treatment
- transformed with the IFS2 transgene driven by the cotyledon-preferable lectin promotere0
- Gene
- IFS2e0
- Outcome
- isoflavone synthesise0
- Direction
- inhibitione0
- Comparator
- untransformed controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nearly complete inhibition of isoflavone synthesis was found in the cotyledons of young seedlings of transgenic plants transformed with the IFS2 transgene driven by the cotyledon-preferable lectin promoter compared with the untransformed control during the 10-day observation period”
t0“Soybean Plant Defense”
Claim · model-written summary
Precursors of isoflavone synthesis accumulated in transgenic cotyledons.
- Taxon
- SoybeanGlycine maxt0
- Organ / stage
- cotyledonse0
- Outcome
- precursors of isoflavone synthesise0
- Direction
- accumulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with the precursors of isoflavone synthesis being accumulated in the cotyledons of transgenic plants.”
t0“Soybean Plant Defense”
Claim · model-written summary
The lectin promoter was inferred to be active during seed germination.
- Taxon
- SoybeanGlycine maxt0
- Gene
- lectin promotere0
- Outcome
- active not only during seed development but also during seed germinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results indicated that the lectin promoter could be active not only during seed development but also during seed germination.”
t0“Soybean Plant Defense”
Claim · model-written summary
Downregulated isoflavone synthesis strongly inhibited pathogen-inducible glyceollin following P. sojae inoculation.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- cotyledonse0
- Treatment
- Downregulation of isoflavone synthesise0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Outcome
- pathogen-inducible glyceolline0
- Direction
- inhibitione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Downregulation of isoflavone synthesis only in the seed or in the whole soybean plant caused a strong inhibition of the pathogen-inducible glyceollin in cotyledons after inoculation with P. sojae”
Claim · model-written summary
Both transgenic lines had increased cotyledon susceptibility to P. sojae relative to untransformed plants.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- both transgenic linese0
- Interactor
- Phytophthora sojaePhytophthora sojaet0pathogen
- Outcome
- susceptibility of the cotyledonse0
- Direction
- increasede0
- Comparator
- untransformed plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased susceptibility of the cotyledons of both transgenic lines to this pathogen compared with inoculated cotyledons of untransformed plants”
t0“Soybean Plant Defense Against Phytophthora sojae”
Claim · model-written summary
Constitutive-transgene stems showed suppressed glyceollin synthesis after M. phaseolina inoculation.
- Taxon
- SoybeanGlycine maxt0
- Organ / stage
- stemse0
- Treatment
- transgenic plants expressing the transgene driven by a constitutive promotere0
- Interactor
- M. phaseolina
Macrophomina phaseolinae0pathogen - Outcome
- glyceollin synthesise0
- Direction
- suppressione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When stems were inoculated with M. phaseolina, suppression of glyceollin synthesis was found only in stems of transgenic plants expressing the transgene driven by a constitutive promoter”
t0“Soybean Plant Defense”
Claim · model-written summary
Constitutive-transgene stems developed more severe M. phaseolina infection.
- Taxon
- SoybeanGlycine maxt0
- Interactor
- Macrophomina phaseolinaMacrophomina phaseolinat0pathogen
- Outcome
- more severe infectione0
- Direction
- more severee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which developed more severe infection.”
t0“Soybean Plant Defense Against Phytophthora sojae and Macrophomina phaseolina”
The same questions in other papers
isoflavone · Phytophthora sojae · Glycine max (soybean)Results agree
2 papers in the same direction as this paper
- negative
- negative
loss of function · Phytophthora sojae · Glycine max (soybean)Positive and negative results
14 papers in the same direction as this paper · 9 in the opposite direction
- positive
- positive
- positive
20 papers more in this group
See all 24 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.