Abstract
To investigate whether the transcriptional response to carbon (C) depletion and sucrose resupply depends on the duration and severity of the C depletion, Arabidopsis seedlings were grown in liquid culture and harvested 3, 6, 12, 24, 48 and 72 h after removing sucrose from the medium and 30 min after resupplying sucrose at each time. Expression profiling revealed early transcriptional inhibition of cell wall synthesis and remodelling of signalling, followed by induction of C recycling and photosynthesis and general inhibition of growth. The temporal sequence differed from the published response to progressive exhaustion of C during a night and extended night in vegetatively growing plants. The response to sucrose readdition was conserved across the C-depletion time course. Intriguingly, the vast majority of rapidly responding transcripts decreased rather than increased. The majority of transcripts that respond rapidly to sucrose and many transcripts that respond during C depletion also decrease after treating seedlings with the transcriptional inhibitor cordycepin A. Comparison with published responses to overexpression of otsA, AKIN10 and bZIP11 revealed that many genes that respond to C depletion, and especially sucrose resupply, respond to one or more of these C-signalling components. Thus, multiple factors contribute to C responsiveness, including many signalling components, transcriptional regulation and transcript turnover.
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Claims 8
Claim · model-written summary
Carbon depletion early inhibited transcription of genes for cell wall synthesis and remodelling of signalling in Arabidopsis seedlings.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- removing sucrose from the mediume0
- Outcome
- cell wall synthesis and remodelling of signallinge1
- Direction
- inhibitione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis seedlings were grown in liquid culture and harvested 3, 6, 12, 24, 48 and 72 h after removing sucrose from the medium”
e1“Expression profiling revealed early transcriptional inhibition of cell wall synthesis and remodelling of signalling”
Claim · model-written summary
After the early phase, carbon depletion induced C recycling and photosynthesis transcripts in Arabidopsis seedlings.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- removing sucrose from the mediume0
- Outcome
- C recycling and photosynthesise2
- Direction
- inductione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis seedlings were grown in liquid culture and harvested 3, 6, 12, 24, 48 and 72 h after removing sucrose from the medium”
e2“followed by induction of C recycling and photosynthesis”
Claim · model-written summary
Carbon depletion generally inhibited growth of Arabidopsis seedlings.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- removing sucrose from the mediume0
- Outcome
- growthe3
- Direction
- inhibitione3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis seedlings were grown in liquid culture and harvested 3, 6, 12, 24, 48 and 72 h after removing sucrose from the medium”
e3“general inhibition of growth”
Claim · model-written summary
The transcriptional response of Arabidopsis seedlings to sucrose readdition was conserved across the carbon-depletion time course.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- response to sucrose readditione4
- Qualifier
- conserved across the C-depletion time coursee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis seedlings were grown in liquid culture and harvested 3, 6, 12, 24, 48 and 72 h after removing sucrose from the medium”
e4“The response to sucrose readdition was conserved across the C-depletion time course”
Claim · model-written summary
The vast majority of rapidly responding transcripts decreased rather than increased after sucrose readdition.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- rapidly responding transcriptse5
- Direction
- decreasede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis seedlings were grown in liquid culture and harvested 3, 6, 12, 24, 48 and 72 h after removing sucrose from the medium”
e5“Intriguingly, the vast majority of rapidly responding transcripts decreased rather than increased”
Claim · model-written summary
Cordycepin A decreased the transcripts that respond rapidly to sucrose and many transcripts that respond during carbon depletion.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- cordycepin Ae6
- Outcome
- transcripts that respond rapidly to sucrosee6
- Direction
- decreasee6
- Qualifier
- many transcripts that respond during C depletione6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis seedlings were grown in liquid culture and harvested 3, 6, 12, 24, 48 and 72 h after removing sucrose from the medium”
e6“The majority of transcripts that respond rapidly to sucrose and many transcripts that respond during C depletion also decrease after treating seedlings with the transcriptional inhibitor cordycepin A”
Claim · model-written summary
Many genes that respond to C depletion respond to one or more of the C-signalling components otsA, AKIN10 and bZIP11.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- otsA, AKIN10 and bZIP11e1
- Outcome
- genes that respond to C depletione7
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis seedlings were grown in liquid culture and harvested 3, 6, 12, 24, 48 and 72 h after removing sucrose from the medium”
e7“many genes that respond to C depletion, and especially sucrose resupply, respond to one or more of these C-signalling components”
e1“overexpression of otsA, AKIN10 and bZIP11”
Claim · model-written summary
Multiple factors contribute to Arabidopsis C responsiveness, including signalling components, transcriptional regulation and transcript turnover.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- C responsivenesse1
- Qualifier
- including many signalling components, transcriptional regulation and transcript turnovere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis seedlings were grown in liquid culture and harvested 3, 6, 12, 24, 48 and 72 h after removing sucrose from the medium”
e1“multiple factors contribute to C responsiveness, including many signalling components, transcriptional regulation and transcript turnover”
The same questions in other papers
sucrose · growth · ArabidopsisPositive and negative results
7 papers in the same direction as this paper · 9 in the opposite direction
- positive
- positive
- positive
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See all 17 papers →in vitro · growth · ArabidopsisPositive and negative results
5 papers in the same direction as this paper · 11 in the opposite direction
- positive
- positive
- positive
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See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.