Abstract
The distribution of protochlorophyllide (Pchlide) and NADPH-Pchlide oxidoreductase (POR) was characterized in the epicotyls and roots of wild-type pea (Pisum sativum L. cv. Alaska) and lip1, a mutant with light-independent photomorphogenesis caused by a mutation in the COP1 locus. The upper part of the dark-grown lip1 mutant epicotyls had a high Pchlide content that decreased downward the organ. The elevated Pchlide level in lip1 seedlings was a result of the differentiation of more proplastids into Pchlide-containing plastids. The cortex cells in the lip1 epicotyl were filled with such plastids in contrast to the cortex cells of wild-type seedlings. The mutant also developed Pchlide-containing plastids in the roots, indicating the suppressing effect of the COP1 locus on development of plastids in the corresponding tissues in dark-grown wild-type plants. The distribution of Pchlide-containing plastids in dark-grown lip1 mutant stem and root was similar to the distribution of chloroplasts in irradiated wild-type plants. Both wild-type and lip1 epicotyls contained mostly short wavelength Pchlide fluorescing at 631 nm with only a small shoulder at 654 nm, which was transformed to a minute amount of chlorophyllide (Chlide) by flash irradiation. In contrast, with continuous irradiation a considerable amount of Chlide was formed especially in the lip1 epicotyls. Immunoblots indicated the presence of POR, as a 36 kDa band, in epicotyls of both dark-grown wild-type and lip1 mutant seedlings. However, lip1 stem tissue had a higher content of POR than the wild-type pea. The high content of POR was unexpected as lip1 lacked both the 654 nm fluorescing Pchlide form and the regular PLBs. In light, a significant amount of chlorophyll was formed also in the roots of the lip1 seedlings.
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Claims 12
Claim · model-written summary
Pchlide content was high at the top of dark-grown lip1 epicotyls and decreased downward.
- Taxon
- pea
Lathyrus oleraceust0 - Material
- lip1e0
- Organ / stage
- epicotylse0
- Outcome
- Pchlide contente0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The upper part of the dark-grown lip1 mutant epicotyls had a high Pchlide content that decreased downward the organ.”
t0“Pea”
Claim · model-written summary
Elevated Pchlide in lip1 seedlings resulted from more proplastids differentiating into Pchlide-containing plastids.
- Taxon
- peaLathyrus oleraceust0
- Material
- lip1e0
- Organ / stage
- seedlingse0
- Outcome
- elevated Pchlide levele0
- Qualifier
- differentiation of more proplastids into Pchlide-containing plastidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The elevated Pchlide level in lip1 seedlings was a result of the differentiation of more proplastids into Pchlide-containing plastids.”
t0“Pea”
Claim · model-written summary
lip1 epicotyl cortex cells contained Pchlide-containing plastids unlike wild-type cells.
- Taxon
- peaLathyrus oleraceust0
- Material
- lip1e0
- Organ / stage
- cortex cellse0
- Outcome
- plastidse0
- Comparator
- wild-type seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cortex cells in the lip1 epicotyl were filled with such plastids in contrast to the cortex cells of wild-type seedlings.”
t0“Pea”
Claim · model-written summary
lip1 roots developed Pchlide-containing plastids, consistent with COP1 suppression of plastid development in dark-grown wild-type plants.
- Taxon
- peaLathyrus oleraceust0
- Material
- mutante0
- Organ / stage
- rootse0
- Gene
- COP1 locuse0
- Outcome
- development of plastidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mutant also developed Pchlide-containing plastids in the roots, indicating the suppressing effect of the COP1 locus on development of plastids in the corresponding tissues in dark-grown wild-type plants.”
t0“Pea”
Claim · model-written summary
The plastid distribution in dark-grown lip1 stems and roots resembled chloroplast distribution in irradiated wild-type plants.
- Taxon
- peaLathyrus oleraceust0
- Material
- lip1e0
- Organ / stage
- stem and roote0
- Outcome
- distribution of Pchlide-containing plastidse0
- Comparator
- irradiated wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The distribution of Pchlide-containing plastids in dark-grown lip1 mutant stem and root was similar to the distribution of chloroplasts in irradiated wild-type plants.”
t0“Pea”
Claim · model-written summary
Both wild-type and lip1 epicotyls mostly contained short-wavelength Pchlide fluorescing at 631 nm, with a small 654 nm shoulder.
- Taxon
- peaLathyrus oleraceust0
- Organ / stage
- epicotylse0
- Outcome
- short wavelength Pchlidee0
- Value
- 631 nme0
- Qualifier
- small shoulder at 654 nme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both wild-type and lip1 epicotyls contained mostly short wavelength Pchlide fluorescing at 631 nm with only a small shoulder at 654 nm”
t0“Pea”
Claim · model-written summary
Flash irradiation transformed little Pchlide into chlorophyllide.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- flash irradiatione0
- Outcome
- chlorophyllide (Chlide)e0
- Direction
- transformede0
- Qualifier
- minute amounte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which was transformed to a minute amount of chlorophyllide (Chlide) by flash irradiation.”
t0“Pea”
Claim · model-written summary
Continuous irradiation formed considerable chlorophyllide, especially in lip1 epicotyls.
- Taxon
- peaLathyrus oleraceust0
- Material
- lip1e0
- Organ / stage
- epicotylse0
- Treatment
- continuous irradiatione0
- Outcome
- Chlidee0
- Direction
- formede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with continuous irradiation a considerable amount of Chlide was formed especially in the lip1 epicotyls.”
t0“Pea”
Claim · model-written summary
POR was present as a 36 kDa band in dark-grown wild-type and lip1 epicotyls.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Immunoblots indicated the presence of POR, as a 36 kDa band, in epicotyls of both dark-grown wild-type and lip1 mutant seedlings.”
t0“Pea”
Claim · model-written summary
lip1 stem tissue had more POR than wild-type pea.
- Taxon
- peaLathyrus oleraceuse0
- Material
- lip1e0
- Organ / stage
- stem tissuee0
- Gene
- PORe0
- Outcome
- content of PORe0
- Direction
- highere0
- Comparator
- wild-type peae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lip1 stem tissue had a higher content of POR than the wild-type pea.”
Claim · model-written summary
lip1 lacked the 654 nm fluorescing Pchlide form and regular PLBs.
- Taxon
- peaLathyrus oleraceust0
- Material
- lip1e0
- Outcome
- 654 nm fluorescing Pchlide form and the regular PLBse0
- Direction
- lackede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lip1 lacked both the 654 nm fluorescing Pchlide form and the regular PLBs.”
t0“Pea”
Claim · model-written summary
Light produced a significant amount of chlorophyll in lip1 roots.
- Taxon
- peaLathyrus oleraceust0
- Material
- lip1e0
- Organ / stage
- rootse0
- Treatment
- lighte0
- Outcome
- chlorophylle0
- Direction
- formede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In light, a significant amount of chlorophyll was formed also in the roots of the lip1 seedlings.”
t0“Pea”
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See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.