Abstract
The present study was designated to explore the effects and physiological mechanism of cold resistance enhanced by different pH buffer in winter wheat.Using Jimai 22 as the material,a pot experiment was conducted to investigate the effects of different pH buffer on MDA content,electrical conductivity,antioxidative enzymes activity,relative water content and stomatal conductance of winter wheat seedlings under low temperature stress for 9 d.The results showed that the electrical conductivity and malondialdehyde(MDA) content in the seedlings increased with the prolongation of low temperature stress.But in the pH 6.5 and pH 6.0-treated winter wheat seedlings,the MDA content and electrical conductivity decreased under low temperature stress comparing with the control,the effects of pH 6.0 treatment was better after 9 d stress.The activities of SOD,POD and CAT increased at the first 1 or 4 days of low temperature stress,and then decreased.The decline of the activities of SOD,POD and CAT in the pH 6.0 treatment was obviously less than that of CK with the most significant differences.The relative water content and stomatal conductance at 6 d after spraying pH 6.0 buffer was 33.19% and 49.49% higher than that of CK,respectively.These suggested that exogenous pH 6.0 buffer could enhance antioxidant ability of enzymatic and non-enzymatic defense system and provided an optimal internal environment for intracellular material metabolism and photosynthesis to maintain the higher level of cold resistance under the followed durative low temperature stress.
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Claims 8
Claim · model-written summary
Seedling electrical conductivity and MDA content increased with prolonged cold stress.
- Taxon
- winter wheat
Triticum aestivumt0 - Treatment
- low temperature stresse0
- Outcome
- electrical conductivity and malondialdehyde(MDA) contente0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the electrical conductivity and malondialdehyde(MDA) content in the seedlings increased with the prolongation of low temperature stress”
t0“winter wheat”
Claim · model-written summary
pH 6.5 buffer reduced MDA content and electrical conductivity under cold stress relative to control.
- Taxon
- winter wheatTriticum aestivume0
- Treatment
- pH 6.5e0
- Outcome
- MDA content and electrical conductivitye0
- Direction
- decreasede0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the pH 6.5 and pH 6.0-treated winter wheat seedlings,the MDA content and electrical conductivity decreased under low temperature stress comparing with the control”
Claim · model-written summary
pH 6.0 buffer reduced MDA content and electrical conductivity under cold stress relative to control.
- Taxon
- winter wheatTriticum aestivume0
- Treatment
- pH 6.0e0
- Outcome
- MDA content and electrical conductivitye0
- Direction
- decreasede0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the pH 6.5 and pH 6.0-treated winter wheat seedlings,the MDA content and electrical conductivity decreased under low temperature stress comparing with the control”
Claim · model-written summary
pH 6.0 treatment performed better after nine days of stress.
- Taxon
- winter wheatTriticum aestivumt0
- Treatment
- pH 6.0 treatmente0
- Outcome
- effects of pH 6.0 treatment was bettere0
- Direction
- bettere0
- Qualifier
- after 9 d stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the effects of pH 6.0 treatment was better after 9 d stress”
t0“winter wheat”
Claim · model-written summary
SOD, POD and CAT activities first increased then decreased under cold stress.
- Taxon
- winter wheatTriticum aestivumt0
- Treatment
- low temperature stresse0
- Gene
- SOD,POD and CATe0
- Outcome
- activities of SOD,POD and CATe0
- Direction
- increased at the first 1 or 4 days of low temperature stress,and then decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activities of SOD,POD and CAT increased at the first 1 or 4 days of low temperature stress,and then decreased.”
t0“winter wheat”
Claim · model-written summary
The decline in SOD, POD and CAT activities was less with pH 6.0 than CK.
- Taxon
- winter wheatTriticum aestivumt0
- Treatment
- pH 6.0 treatmente0
- Outcome
- activities of SOD,POD and CATe0
- Direction
- lesse0
- Comparator
- CKe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The decline of the activities of SOD,POD and CAT in the pH 6.0 treatment was obviously less than that of CK with the most significant differences.”
t0“winter wheat”
Claim · model-written summary
Relative water content was 33.19 percent higher six days after pH 6.0 spraying than CK.
- Taxon
- winter wheatTriticum aestivumt0
- Treatment
- spraying pH 6.0 buffere0
- Outcome
- relative water contente0
- Direction
- highere0
- Value
- 33.19%e0
- Comparator
- CKe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The relative water content and stomatal conductance at 6 d after spraying pH 6.0 buffer was 33.19% and 49.49% higher than that of CK,respectively.”
t0“winter wheat”
Claim · model-written summary
Stomatal conductance was 49.49 percent higher six days after pH 6.0 spraying than CK.
- Taxon
- winter wheatTriticum aestivumt0
- Treatment
- spraying pH 6.0 buffere0
- Outcome
- stomatal conductancee0
- Direction
- highere0
- Value
- 49.49%e0
- Comparator
- CKe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The relative water content and stomatal conductance at 6 d after spraying pH 6.0 buffer was 33.19% and 49.49% higher than that of CK,respectively.”
t0“winter wheat”
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