Abstract
The aim of the present research is to observe the effect of sucrose level (0 – 6%) in the nutrient medium at the elongation stage on the rooting ability of in vitro cultivated apple rootstock M 26 (Malus domestica Borkh). The source microplants are maintained on a modified MS multiplication medium (5 μM BAP, 30 g/l sucrose and 5.8 g/l agar). For the present experiment the plantlets are transferred for 14 days on an elongation (hormone-free medium) with the same mineral content with different sucrose concentrations 0, 1, 2, 4 and 6% w/v. They are cultivated in polypropylene vessels with gas-permeable closure (Combiness, Belgium, gas exchange rate – 10 GE/day) for 14 days. After that shoots 20 mm high are set for rooting in the same vessels on two types carbohydrate free medium – solidified with agar or liquid with perlite. Rooting is evaluated after 20 days. The content of glucose, fructose, sucrose, sorbitol and starch in the plants elongated on media with different sucrose concentrations is determined before transferring on rooting media. The increase in the sucrose concentration in the elongation medium (0 – 6%) leads to different content of soluble carbohydrates and starch, especially at the extreme concentrations of 0 and 6%. On a sugar-free nutritional medium (photoautotrophic variant) sorbitol predominates and this confirm the photosynthetic competence of the apple plants in vitro. At the highest sucrose concentration (6%) in the elongation medium accumulation of glucose, sucrose and starch in plantlets is observed, but these do not improve the rooting on carbohydrate – free medium. Elongation on the medium with 4% sucrose stimulates rooting on the agar based sucrose-free medium. When rooting is carried out in perlite based liquid medium, rooting percent is in the range of 50 – 65% and the sucrose concentration at elongation stage has no significant effect. The obtained results show some technological approaches for improving the rooting of micropropagated apple rootstock M 26 (Malus domestica Borkh).
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Claims 8
Claim · model-written summary
Sucrose concentration during elongation changed soluble carbohydrate and starch contents, especially at 0 and 6%.
- Taxon
- apple
Malus domesticat0 - Material
- M 26t0
- Treatment
- sucrose concentration in the elongation medium (0 – 6%)e0
- Outcome
- content of soluble carbohydrates and starche0
- Comparator
- 0 and 6%e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The increase in the sucrose concentration in the elongation medium (0 – 6%) leads to different content of soluble carbohydrates and starch, especially at the extreme concentrations of 0 and 6%.”
t0“apple rootstock M 26”
Claim · model-written summary
Sorbitol predominated in plantlets elongated on sugar-free medium.
- Taxon
- appleMalus domesticae0
- Treatment
- sugar-free nutritional mediume0
- Outcome
- sorbitol predominatese0
- Direction
- predominatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On a sugar-free nutritional medium (photoautotrophic variant) sorbitol predominates and this confirm the photosynthetic competence of the apple plants in vitro.”
Claim · model-written summary
The authors interpreted sorbitol predominance as confirmation of photosynthetic competence in vitro.
- Taxon
- appleMalus domesticae0
- Outcome
- photosynthetic competencee0
- Qualifier
- sorbitol predominatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On a sugar-free nutritional medium (photoautotrophic variant) sorbitol predominates and this confirm the photosynthetic competence of the apple plants in vitro.”
Claim · model-written summary
Plantlets accumulated glucose, sucrose and starch at 6% elongation sucrose.
- Taxon
- appleMalus domesticat0
- Treatment
- highest sucrose concentration (6%)e0
- Outcome
- accumulation of glucose, sucrose and starch in plantletse0
- Direction
- accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At the highest sucrose concentration (6%) in the elongation medium accumulation of glucose, sucrose and starch in plantlets is observed”
t0“apple rootstock M 26”
Claim · model-written summary
The carbohydrate accumulation at 6% sucrose did not improve rooting on carbohydrate-free medium.
- Taxon
- appleMalus domesticat0
- Treatment
- highest sucrose concentration (6%)e0
- Outcome
- rootinge0
- Direction
- do not improvee0
- Qualifier
- carbohydrate – free mediume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At the highest sucrose concentration (6%) in the elongation medium accumulation of glucose, sucrose and starch in plantlets is observed, but these do not improve the rooting on carbohydrate – free medium.”
t0“apple rootstock M 26”
Claim · model-written summary
Elongation at 4% sucrose stimulated rooting on agar-based sucrose-free medium.
- Taxon
- appleMalus domesticat0
- Treatment
- medium with 4% sucrosee0
- Outcome
- rootinge0
- Direction
- stimulatese0
- Qualifier
- agar based sucrose-free mediume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Elongation on the medium with 4% sucrose stimulates rooting on the agar based sucrose-free medium.”
t0“apple rootstock M 26”
Claim · model-written summary
Rooting in perlite-based liquid medium was 50 – 65%.
- Taxon
- appleMalus domesticat0
- Treatment
- perlite based liquid mediume0
- Outcome
- rooting percente0
- Value
- 50 – 65%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When rooting is carried out in perlite based liquid medium, rooting percent is in the range of 50 – 65%”
t0“apple rootstock M 26”
Claim · model-written summary
Elongation sucrose concentration had no significant effect on rooting in perlite-based liquid medium.
- Taxon
- appleMalus domesticat0
- Treatment
- sucrose concentration at elongation stagee0
- Outcome
- rooting percente0
- Direction
- no significant effecte0
- Qualifier
- perlite based liquid mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When rooting is carried out in perlite based liquid medium, rooting percent is in the range of 50 – 65% and the sucrose concentration at elongation stage has no significant effect.”
t0“apple rootstock M 26”
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sucrose · growth · Malus domestica (apple)Positive and negative results
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See all 38 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.