Abstract
C2H2-type zinc finger transcription factor sensitive to proton rhizotoxicity 1 (STOP1) plays an essential role in aluminium (Al) resistance in Arabidopsis thaliana by controlling the expression of a set of Al-resistance genes, including the malate transporter-encoding gene A. thaliana aluminium activated malate transporter 1 (AtALMT1) that is critically required for Al resistance. STOP1 is suggested to be modulated by Al at post-transcriptional and/or post-translational levels. However, the underlying molecular mechanisms remain to be demonstrated. We carried out a forward genetic screen on an ethyl methanesulphonate mutagenized population, which contains the AtALMT1 promoter-driven luciferase reporter gene (pAtALMT1:LUC), and identified hyperrecombination protein 1 (HPR1), which encodes a subunit of the THO/TREX complex. We investigate the effect of hpr1 mutations on the expression of Al-resistance genes and Al resistance, and we also examined the regulatory role of HPR1 in nuclear messenger RNA (mRNA) and protein accumulation of STOP1 gene. Mutation of HPR1 reduces the expression of STOP1-regulated genes and the associated Al resistance. The hpr1 mutations increase STOP1 mRNA retention in the nucleus and consequently decrease STOP1 protein abundance. Mutation of regulation of AtALMT1 expression 1 (RAE1) that mediates STOP1 degradation in the hpr1 mutant background can partially rescue the deficient phenotypes of hpr1 mutants. Our results demonstrate that HPR1 modulates Al resistance partly through the regulation of nucleocytoplasmic STOP1 mRNA export.
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Claims 7
Claim · model-written summary
A forward genetic screen identified HPR1, encoding a THO/TREX subunit.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Gene
- hyperrecombination protein 1 (HPR1)e0
- Outcome
- encodes a subunit of the THO/TREX complexe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified hyperrecombination protein 1 (HPR1), which encodes a subunit of the THO/TREX complex”
t0“Arabidopsis thaliana”
Claim · model-written summary
HPR1 mutation reduced STOP1-regulated gene expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Mutation of HPR1e0
- Gene
- HPR1e0
- Outcome
- expression of STOP1-regulated genese0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation of HPR1 reduces the expression of STOP1-regulated genes and the associated Al resistance.”
t0“Arabidopsis thaliana”
Claim · model-written summary
HPR1 mutation reduced aluminium resistance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Mutation of HPR1e0
- Gene
- HPR1e0
- Outcome
- Al resistancee0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation of HPR1 reduces the expression of STOP1-regulated genes and the associated Al resistance.”
t0“Arabidopsis thaliana”
Claim · model-written summary
hpr1 mutations increased nuclear retention of STOP1 mRNA.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- hpr1 mutationse0
- Gene
- STOP1e0
- Outcome
- STOP1 mRNA retention in the nucleuse0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The hpr1 mutations increase STOP1 mRNA retention in the nucleus and consequently decrease STOP1 protein abundance.”
t0“Arabidopsis thaliana”
Claim · model-written summary
hpr1 mutations consequently decreased STOP1 protein abundance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- hpr1 mutationse0
- Gene
- STOP1e0
- Outcome
- STOP1 protein abundancee0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The hpr1 mutations increase STOP1 mRNA retention in the nucleus and consequently decrease STOP1 protein abundance.”
t0“Arabidopsis thaliana”
Claim · model-written summary
RAE1 mutation in hpr1 partially rescued deficient phenotypes.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Mutation of regulation of AtALMT1 expression 1 (RAE1)e0
- Gene
- regulation of AtALMT1 expression 1 (RAE1)e0
- Outcome
- deficient phenotypese0
- Direction
- rescuee0
- Qualifier
- partiallye0
- Comparator
- hpr1 mutantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation of regulation of AtALMT1 expression 1 (RAE1) that mediates STOP1 degradation in the hpr1 mutant background can partially rescue the deficient phenotypes of hpr1 mutants.”
t0“Arabidopsis thaliana”
Claim · model-written summary
HPR1 partly modulates aluminium resistance through nucleocytoplasmic STOP1 mRNA export.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- HPR1e0
- Outcome
- Al resistancee0
- Qualifier
- partly through the regulation of nucleocytoplasmic STOP1 mRNA exporte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our results demonstrate that HPR1 modulates Al resistance partly through the regulation of nucleocytoplasmic STOP1 mRNA export.”
t0“Arabidopsis thaliana”
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