Abstract
Abstract Background Global warming threatens rice growth and reduces yields. Proline plays important roles in plant abiotic stress tolerance. Previous research demonstrated that engineering proline metabolism-related genes can enhance tolerance to freezing and salinity in Arabidopsis . OsProDH encodes a putative proline dehydrogenase and is a single copy gene in rice. However, whether OsProDH plays roles in abiotic stress in rice remains unknown. Findings Quantitative RT-PCR analysis revealed that OsProDH transcript contents were relatively higher in leaf blade and root tissues and the high temperature treatment repressed expression of OsProDH . The predicted OsProDH protein localized in mitochondria. Using the Oryza sativa ssp. japonica cultivar KY131, we generated OsProDH overexpression (OE) lines and knockout mutant lines using the CRISPR/Cas9 (CRI) system. Overexpression of OsProDH decreased proline content, while mutation of OsProDH increased proline content compared with that of KY131. The CRI and OE lines were respectively more resistant and sensitive to heat stress than KY131. Heat stress induced proline accumulation and mutation of OsProDH led to proline overproduction which reduced H 2 O 2 accumulation in the seedlings. Conclusions OsProDH negatively regulates thermotolerance in rice. Our study provides a strategy to improve heat tolerance in rice via manipulating proline metabolism.
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Claims 11
Claim · model-written summary
OsProDH transcript contents were relatively higher in leaf blade and root tissues.
- Taxon
- rice
Oryza sativae0 - Organ / stage
- leaf blade and root tissuese1
- Gene
- OsProDHe1
- Outcome
- OsProDH transcript contentse1
- Direction
- relatively highere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Global warming threatens rice growth and reduces yields”
e1“Quantitative RT-PCR analysis revealed that OsProDH transcript contents were relatively higher in leaf blade and root tissues”
Claim · model-written summary
High temperature treatment repressed expression of OsProDH.
- Taxon
- riceOryza sativae0
- Treatment
- high temperaturee1
- Gene
- OsProDHe1
- Outcome
- expression of OsProDHe1
- Direction
- repressede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Global warming threatens rice growth and reduces yields”
e1“the high temperature treatment repressed expression of OsProDH”
Claim · model-written summary
The predicted OsProDH protein is localized in mitochondria.
- Taxon
- riceOryza sativae0
- Gene
- OsProDH proteine1
- Outcome
- localized in mitochondriae1
- Qualifier
- predictede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Global warming threatens rice growth and reduces yields”
e1“The predicted OsProDH protein localized in mitochondria”
Claim · model-written summary
Overexpression of OsProDH decreased proline content compared with KY131.
- Taxon
- riceOryza sativae0
- Material
- KY131e1
- Treatment
- Overexpression of OsProDHe1
- Gene
- OsProDHe1
- Outcome
- proline contente1
- Direction
- decreasede1
- Comparator
- KY131e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Global warming threatens rice growth and reduces yields”
e1“Overexpression of OsProDH decreased proline content, while mutation of OsProDH increased proline content compared with that of KY131”
Claim · model-written summary
Mutation of OsProDH increased proline content compared with KY131.
- Taxon
- riceOryza sativae0
- Material
- KY131e1
- Treatment
- mutation of OsProDHe1
- Gene
- OsProDHe1
- Outcome
- proline contente1
- Direction
- increasede1
- Comparator
- KY131e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Global warming threatens rice growth and reduces yields”
e1“Overexpression of OsProDH decreased proline content, while mutation of OsProDH increased proline content compared with that of KY131”
Claim · model-written summary
CRI lines were more resistant to heat stress than KY131.
- Taxon
- riceOryza sativae0
- Material
- CRIe1
- Treatment
- heat stresse1
- Outcome
- resistante1
- Direction
- more resistante1
- Comparator
- KY131e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Global warming threatens rice growth and reduces yields”
e1“The CRI and OE lines were respectively more resistant and sensitive to heat stress than KY131”
Claim · model-written summary
OE lines were more sensitive to heat stress than KY131.
- Taxon
- riceOryza sativae0
- Material
- OEe1
- Treatment
- heat stresse1
- Outcome
- sensitivee1
- Direction
- sensitivee1
- Comparator
- KY131e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Global warming threatens rice growth and reduces yields”
e1“The CRI and OE lines were respectively more resistant and sensitive to heat stress than KY131”
Claim · model-written summary
Heat stress induced proline accumulation in rice seedlings.
- Taxon
- riceOryza sativae0
- Treatment
- Heat stresse1
- Compound
- prolinee1
- Outcome
- proline accumulatione1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Global warming threatens rice growth and reduces yields”
e1“Heat stress induced proline accumulation”
Claim · model-written summary
Mutation of OsProDH led to proline overproduction, which reduced H 2 O 2 accumulation in seedlings.
- Taxon
- riceOryza sativae0
- Organ / stage
- seedlingse1
- Treatment
- mutation of OsProDHe1
- Gene
- OsProDHe1
- Compound
- prolinee1
- Outcome
- H 2 O 2 accumulatione1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Global warming threatens rice growth and reduces yields”
e1“mutation of OsProDH led to proline overproduction which reduced H 2 O 2 accumulation in the seedlings”
Claim · model-written summary
OsProDH negatively regulates thermotolerance in rice.
- Taxon
- riceOryza sativae0
- Gene
- OsProDHe0
- Outcome
- thermotolerancee0
- Qualifier
- negatively regulatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsProDH negatively regulates thermotolerance in rice”
Claim · model-written summary
Manipulating proline metabolism is a strategy to improve heat tolerance in rice.
- Taxon
- riceOryza sativae0
- Treatment
- manipulating proline metabolisme0
- Outcome
- heat tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our study provides a strategy to improve heat tolerance in rice via manipulating proline metabolism”
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