Abstract
The effects of combined application of 3, 4-dimethylpyrazole phosphate (DMPP) and corn-stalk biochar on nitrogen transformation and nitrogen use efficiency in acid and neutral paddy soils were investigated by pot experiments. Four treatments were set up as follows: control (CK), DMPP, DMPP+300 ℃ biochar (DMPP+300BC), DMPP+500 ℃ biochar (DMPP+500BC). The results showed that compared with the DMPP treatment, DMPP combined with 500 ℃ biochar significantly increased rice grain yield and nitrogen use efficiency in the neutral paddy soil by 8.5% and 10.6% (P<0.05), respectively, but had no significant effect in the acid paddy soil. DMPP combined with biochar could effectively prolong the duration of nitrification inhibition. After 42 d of rice culture, the ammonium nitrogen (<alternatives>NH4+<inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/43C56AB2-4347-40ec-A968-18C889111740-M004.jpg"/></alternatives>-N) content in the two paddy soils was higher than that of the DMPP treatment. DMPP combined with biochar could effectively inhibit the transformation of <alternatives>NH4+<inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/43C56AB2-4347-40ec-A968-18C889111740-M005.jpg"/></alternatives>-N to nitrate nitrogen (<alternatives>NO3-<inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/43C56AB2-4347-40ec-A968-18C889111740-M006.jpg"/></alternatives>-N), and the effect in the neutral paddy soil was better than that in the acid paddy soil, and the effect of 500 ℃ biochar was better than that of 300 ℃ biochar. After 21 d of rice culture, DMPP combined with 300 ℃ and 500 ℃ biochars reduced the denitrification activities of acid paddy soil by 45.4% and 80.9%, respectively, which were better than those of neutral paddy soil. Within 21 d of rice culture, DMPP combined with biochar had no significant effect on the abundance of ammonia oxidizing archaea (AOA) in the two paddy soils, but increased the abundance of ammonia oxidizing bacteria (AOB) in the acid paddy soil, while decreased the abundance of AOB in the neutral paddy soil, as compared with the DMPP treatment. In conclusion, DMPP combined with 500 ℃ biochar can inhibit nitrification by prolonging the duration of nitrification inhibition and reducing the abundance of AOB in the neutral paddy soil, thus improve rice grain yield and nitrogen use efficiency.
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Claims 11
Claim · model-written summary
500 ℃ biochar with DMPP raised rice grain yield 8.5% against DMPP in neutral soil.
- Taxon
- rice
Oryza sativae0
- Treatment
- DMPP combined with 500 ℃ biochare0
- Outcome
- rice grain yielde0
- Direction
- increasede0
- Value
- 8.5%e0
- Comparator
- DMPPe0
- Setting
- neutral paddy soile0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DMPP combined with 500 ℃ biochar significantly increased rice grain yield and nitrogen use efficiency in the neutral paddy soil by 8.5% and 10.6% (P<0.05), respectively”
t0“paddy soil”
Claim · model-written summary
500 ℃ biochar with DMPP raised rice nitrogen use efficiency 10.6% against DMPP in neutral soil.
- Taxon
- rice
Oryza sativae0
- Treatment
- DMPP combined with 500 ℃ biochare0
- Outcome
- nitrogen use efficiencye0
- Direction
- increasede0
- Value
- 10.6%e0
- Comparator
- DMPPe0
- Setting
- neutral paddy soile0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DMPP combined with 500 ℃ biochar significantly increased rice grain yield and nitrogen use efficiency in the neutral paddy soil by 8.5% and 10.6% (P<0.05), respectively”
Claim · model-written summary
DMPP with biochar prolonged nitrification inhibition.
- Treatment
- DMPP combined with biochare0
- Outcome
- duration of nitrification inhibitione0
- Direction
- prolonge0
Not reportedTaxonComparator
Verbatim
e0“DMPP combined with biochar could effectively prolong the duration of nitrification inhibition”
t0“paddy soil”
Claim · model-written summary
At 42 days, soil ammonium nitrogen was higher under the combination than DMPP alone.
- Taxon
- rice
Oryza sativae0
- Outcome
- ammonium nitrogene0
- Direction
- highere1
- Qualifier
- After 42 d of rice culturee0
- Comparator
- DMPP treatmente1
Not reportedTreatment
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After 42 d of rice culture, the ammonium nitrogen”
e1“-N) content in the two paddy soils was higher than that of the DMPP treatment”
Claim · model-written summary
The combination inhibited conversion of ammonium to nitrate, more in neutral than acid soil.
- Treatment
- DMPP combined with biochare0
- Outcome
- transformatione0
- Direction
- inhibite0
- Comparator
- acid paddy soile1
Not reportedTaxon
Verbatim
e0“DMPP combined with biochar could effectively inhibit the transformation of”
e1“and the effect in the neutral paddy soil was better than that in the acid paddy soil”
t0“paddy soil”
Claim · model-written summary
500 ℃ biochar had a stronger inhibition effect than 300 ℃ biochar.
Not reportedTaxon
Verbatim
e0“the effect of 500 ℃ biochar was better than that of 300 ℃ biochar”
t0“paddy soil”
Claim · model-written summary
300 ℃ biochar with DMPP reduced acid-soil denitrification activity by 45.4%.
- Treatment
- DMPP combined with 300 ℃e0
- Outcome
- denitrification activities of acid paddy soile0
- Direction
- reducede0
- Value
- 45.4%e0
Not reportedTaxonComparator
Verbatim
e0“DMPP combined with 300 ℃ and 500 ℃ biochars reduced the denitrification activities of acid paddy soil by 45.4% and 80.9%, respectively”
t0“paddy soil”
Claim · model-written summary
500 ℃ biochar with DMPP reduced acid-soil denitrification activity by 80.9%.
- Treatment
- DMPP combined with 300 ℃ and 500 ℃ biocharse0
- Outcome
- denitrification activities of acid paddy soile0
- Direction
- reducede0
- Value
- 80.9%e0
Not reportedTaxonComparator
Verbatim
e0“DMPP combined with 300 ℃ and 500 ℃ biochars reduced the denitrification activities of acid paddy soil by 45.4% and 80.9%, respectively”
t0“paddy soil”
Claim · model-written summary
The combination had no significant effect on ammonia oxidizing archaea abundance.
- Treatment
- DMPP combined with biochare0
- Outcome
- abundance of ammonia oxidizing archaea (AOA)e0
- Direction
- no significant effecte0
Not reportedTaxonComparator
Verbatim
e0“DMPP combined with biochar had no significant effect on the abundance of ammonia oxidizing archaea (AOA) in the two paddy soils”
Claim · model-written summary
The combination increased ammonia oxidizing bacteria in acid soil.
Not reportedTaxonTreatmentComparator
Verbatim
e0“increased the abundance of ammonia oxidizing bacteria (AOB) in the acid paddy soil”
Claim · model-written summary
The combination decreased ammonia oxidizing bacteria in neutral soil.
Not reportedTaxonTreatment
Verbatim
e0“decreased the abundance of AOB in the neutral paddy soil, as compared with the DMPP treatment”
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