Abstract
The NRC immune receptor network has evolved in asterid plants from a pair of linked genes into a genetically dispersed and phylogenetically structured network of sensor and helper NLR (nucleotide-binding domain and leucine-rich repeat-containing) proteins. In some species, such as the model plant Nicotiana benthamiana and other Solanaceae, the NRC (NLR-REQUIRED FOR CELL DEATH) network forms up to half of the NLRome, and NRCs are scattered throughout the genome in gene clusters of varying complexities. Here, we describe NRCX, an atypical member of the NRC family that lacks canonical features of these NLR helper proteins, such as a functional N-terminal MADA motif and the capacity to trigger autoimmunity. In contrast to other NRCs, systemic gene silencing of NRCX in N. benthamiana markedly impairs plant growth resulting in a dwarf phenotype. Remarkably, dwarfism of NRCX silenced plants is partially dependent on NRCX paralogs NRC2 and NRC3, but not NRC4. Despite its negative impact on plant growth when silenced systemically, spot gene silencing of NRCX in mature N. benthamiana leaves doesn't result in visible cell death phenotypes. However, alteration of NRCX expression modulates the hypersensitive response mediated by NRC2 and NRC3 in a manner consistent with a negative role for NRCX in the NRC network. We conclude that NRCX is an atypical member of the NRC network that has evolved to contribute to the homeostasis of this genetically unlinked NLR network.
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Claims 8
Claim · model-written summary
NRCX lacks a functional N-terminal MADA motif and capacity to trigger autoimmunity.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Gene
- NRCXe0
- Outcome
- lacks canonical features of these NLR helper proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NRCX, an atypical member of the NRC family that lacks canonical features of these NLR helper proteins, such as a functional N-terminal MADA motif and the capacity to trigger autoimmunity”
t0“Nicotiana benthamiana”
Claim · model-written summary
Systemic NRCX silencing markedly impairs growth and produces dwarf plants.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- systemic gene silencing of NRCXe0
- Gene
- NRCXe0
- Outcome
- plant growthe0
- Direction
- impairse0
- Qualifier
- dwarf phenotypee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“systemic gene silencing of NRCX in N. benthamiana markedly impairs plant growth resulting in a dwarf phenotype”
Claim · model-written summary
NRC2 and NRC3 partially determine dwarfism following NRCX silencing.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NRC2 and NRC3e0
- Outcome
- dwarfisme0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dwarfism of NRCX silenced plants is partially dependent on NRCX paralogs NRC2 and NRC3, but not NRC4”
t0“Nicotiana benthamiana”
Claim · model-written summary
NRC4 does not contribute to dwarfism following NRCX silencing.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NRC4e0
- Outcome
- dwarfisme0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dwarfism of NRCX silenced plants is partially dependent on NRCX paralogs NRC2 and NRC3, but not NRC4”
t0“Nicotiana benthamiana”
Claim · model-written summary
Spot NRCX silencing in mature leaves did not yield visible cell death.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Organ / stage
- mature N. benthamiana leavese0
- Treatment
- spot gene silencing of NRCXe0
- Gene
- NRCXe0
- Outcome
- visible cell death phenotypese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“spot gene silencing of NRCX in mature N. benthamiana leaves doesn't result in visible cell death phenotypes”
Claim · model-written summary
Altered NRCX expression modulates NRC2-mediated hypersensitive response.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NRCXe0
- Outcome
- hypersensitive response mediated by NRC2e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“alteration of NRCX expression modulates the hypersensitive response mediated by NRC2 and NRC3”
t0“Nicotiana benthamiana”
Claim · model-written summary
Altered NRCX expression modulates NRC3-mediated hypersensitive response.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NRCXe0
- Outcome
- hypersensitive response mediated by NRC2 and NRC3e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“alteration of NRCX expression modulates the hypersensitive response mediated by NRC2 and NRC3”
t0“Nicotiana benthamiana”
Claim · model-written summary
The authors infer NRCX has a negative regulatory role in the NRC network.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NRCXe0
- Outcome
- NRC networke0
- Qualifier
- negative rolee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a negative role for NRCX in the NRC network”
t0“Nicotiana benthamiana”
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