Abstract
P0 proteins encoded by the pepper vein yellow virus (PeVYV) are pathogenic factors that cause hypersensitive response (HR). However, the host gene expression related to PeVYV P0-induced HR has not been thoroughly studied. Transcriptomic technology was used to investigate the host pathways mediated by the PeVYV P0 protein to explore the molecular mechanisms underlying its function. We found 12,638 differentially expressed genes (DEGs); 6784 and 5854 genes were significantly upregulated and downregulated, respectively. Transcriptomic and reverse-transcription quantitative polymerase chain reaction (RT-qPCR) analyses revealed that salicylic acid (SA) and jasmonic acid (JA) synthesis-related gene expression was upregulated, and ethylene synthesis-related gene expression was downregulated. Ultrahigh performance liquid chromatography-tandem mass spectrometry was used to quantify SA and JA concentrations in Nicotiana benthamiana, and the P0 protein induced SA and JA biosynthesis. We then hypothesized that the pathogenic activity of the P0 protein might be owing to proteins related to host hormones in the SA and JA pathways, modulating host resistance at different times. Viral gene silencing suppression technology was used in N. benthamiana to characterize candidate proteins, and downregulating NbHERC3 (Homologous to E6-AP carboxy-terminus domain and regulator of choromosome condensation-1 dmain protein 3) accelerated cell necrosis in the host. The downregulation of NbCRR reduced cell death, while that of NbBax induced necrosis and curled heart leaves. Our findings indicate that NbHERC3, NbBax, and NbCRR are involved in P0 protein-driven cell necrosis.
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Claims 9
Claim · model-written summary
P0 exposure yielded 12,638 differentially expressed host genes.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae1 - Interactor
- pepper vein yellow virust0pathogen
- Outcome
- differentially expressed genese0
- Value
- 12,638e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We found 12,638 differentially expressed genes (DEGs); 6784 and 5854 genes were significantly upregulated and downregulated, respectively.”
t0“pepper vein yellow virus P0 protein triggers NbHERC3, NbBax, and NbCRR mediated hypersensitive response”
e1“in Nicotiana benthamiana”
Claim · model-written summary
P0 upregulated 6784 host genes.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Outcome
- genese0
- Direction
- upregulatede0
- Value
- 6784e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“6784 and 5854 genes were significantly upregulated and downregulated, respectively.”
t0“in Nicotiana benthamiana”
e1“in Nicotiana benthamiana”
Claim · model-written summary
P0 downregulated 5854 host genes.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Outcome
- genese0
- Direction
- downregulatede0
- Value
- 5854e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“6784 and 5854 genes were significantly upregulated and downregulated, respectively.”
t0“in Nicotiana benthamiana”
e1“in Nicotiana benthamiana”
Claim · model-written summary
Salicylic and jasmonic acid synthesis gene expression rose.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Outcome
- salicylic acid (SA) and jasmonic acid (JA) synthesis-related gene expressione0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“salicylic acid (SA) and jasmonic acid (JA) synthesis-related gene expression was upregulated”
t0“in Nicotiana benthamiana”
e1“in Nicotiana benthamiana”
Claim · model-written summary
Ethylene synthesis gene expression fell.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Outcome
- ethylene synthesis-related gene expressione0
- Direction
- downregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ethylene synthesis-related gene expression was downregulated.”
t0“in Nicotiana benthamiana”
e1“in Nicotiana benthamiana”
Claim · model-written summary
P0 induced salicylic and jasmonic acid biosynthesis.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- P0 proteine0
- Outcome
- SA and JA biosynthesise0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the P0 protein induced SA and JA biosynthesis.”
t0“in Nicotiana benthamiana”
e1“in Nicotiana benthamiana”
Claim · model-written summary
NbHERC3 downregulation accelerated host cell necrosis.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- downregulating NbHERC3e0
- Gene
- NbHERC3e0
- Outcome
- cell necrosis in the hoste0
- Direction
- acceleratede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“downregulating NbHERC3 (Homologous to E6-AP carboxy-terminus domain and regulator of choromosome condensation-1 dmain protein 3) accelerated cell necrosis in the host.”
t0“in Nicotiana benthamiana”
e1“in Nicotiana benthamiana”
Claim · model-written summary
NbCRR downregulation reduced cell death.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- downregulation of NbCRRe0
- Gene
- NbCRRe0
- Outcome
- cell deathe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The downregulation of NbCRR reduced cell death”
t0“in Nicotiana benthamiana”
e1“in Nicotiana benthamiana”
Claim · model-written summary
NbBax downregulation induced necrosis and curled heart leaves.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbBaxe0
- Gene
- NbBaxe0
- Outcome
- necrosis and curled heart leavese0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“that of NbBax induced necrosis and curled heart leaves.”
t0“in Nicotiana benthamiana”
e1“in Nicotiana benthamiana”
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