Abstract
Efficient uptake, translocation, and distribution of Cu to rice (Oryza sativa) spikelets is crucial for flowering and yield production. However, the regulatory factors involved in this process remain unidentified. In this study, we isolated a WRKY transcription factor gene induced by Cu deficiency, OsWRKY37, and characterized its regulatory role in Cu uptake and transport in rice. OsWRKY37 was highly expressed in rice roots, nodes, leaf vascular bundles, and anthers. Overexpression of OsWRKY37 promoted the uptake and root-to-shoot translocation of Cu in rice under -Cu condition but not under +Cu condition. While mutation of OsWRKY37 significantly decreased Cu concentrations in the stamen, the root-to-shoot translocation and distribution ratio in brown rice affected pollen development, delayed flowering time, decreased fertility, and reduced grain yield under -Cu condition. yeast one-hybrid, transient co-expression and EMSAs, together with in situ RT-PCR and RT-qPCR analysis, showed that OsWRKY37 could directly bind to the upstream promoter region of OsCOPT6 (copper transporter) and OsYSL16 (yellow stripe-like protein) and positively activate their expression levels. Analyses of oscopt6 mutants further validated its important role in Cu uptake in rice. Our study demonstrated that OsWRKY37 acts as a positive regulator involved in the uptake, root-to-shoot translocation, and distribution of Cu through activating the expression of OsCOPT6 and OsYSL16, which is important for pollen development, flowering, fertility, and grain yield in rice under Cu deficient conditions. Our results provide a genetic strategy for improving rice yield under Cu deficient condition.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 17
Claim · model-written summary
OsWRKY37 is a WRKY transcription factor gene induced by copper deficiency.
- Taxon
- Rice
Oryza sativat0 - Treatment
- Cu deficiencye0
- Gene
- OsWRKY37e0
- Outcome
- WRKY transcription factor genee0
- Direction
- inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice transcription factor OsWRKY37”
e0“a WRKY transcription factor gene induced by Cu deficiency, OsWRKY37”
Claim · model-written summary
OsWRKY37 is highly expressed in rice roots, nodes and leaf vascular bundles.
- Taxon
- RiceOryza sativat0
- Organ / stage
- roots, nodes, leaf vascular bundlese1
- Gene
- OsWRKY37e1
- Outcome
- highly expressede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice transcription factor OsWRKY37”
e1“OsWRKY37 was highly expressed in rice roots, nodes, leaf vascular bundles, and anthers”
Claim · model-written summary
OsWRKY37 is highly expressed in rice anthers.
- Taxon
- RiceOryza sativat0
- Organ / stage
- antherse1
- Gene
- OsWRKY37e1
- Outcome
- highly expressede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice transcription factor OsWRKY37”
e1“OsWRKY37 was highly expressed in rice roots, nodes, leaf vascular bundles, and anthers”
Claim · model-written summary
Overexpression of OsWRKY37 promoted copper uptake and root-to-shoot translocation under -Cu condition.
- Taxon
- RiceOryza sativat0
- Treatment
- Overexpression of OsWRKY37e2
- Gene
- OsWRKY37e2
- Outcome
- uptake and root-to-shoot translocatione2
- Direction
- promotede2
- Qualifier
- under -Cu conditione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice transcription factor OsWRKY37”
e2“Overexpression of OsWRKY37 promoted the uptake and root-to-shoot translocation of Cu in rice under -Cu condition but not under +Cu condition”
Claim · model-written summary
Overexpression of OsWRKY37 did not change copper uptake or root-to-shoot translocation under +Cu condition.
- Taxon
- RiceOryza sativat0
- Treatment
- Overexpression of OsWRKY37e2
- Gene
- OsWRKY37e2
- Outcome
- uptake and root-to-shoot translocatione2
- Direction
- no_changeinferrede2
- Qualifier
- under +Cu conditione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice transcription factor OsWRKY37”
e2“Overexpression of OsWRKY37 promoted the uptake and root-to-shoot translocation of Cu in rice under -Cu condition but not under +Cu condition”
Claim · model-written summary
Mutation of OsWRKY37 significantly decreased copper concentrations in the stamen.
- Taxon
- RiceOryza sativat0
- Organ / stage
- stamene3
- Treatment
- mutation of OsWRKY37e3
- Gene
- OsWRKY37e3
- Outcome
- Cu concentrations in the stamene3
- Direction
- decreasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice transcription factor OsWRKY37”
e3“While mutation of OsWRKY37 significantly decreased Cu concentrations in the stamen, the root-to-shoot translocation and distribution ratio in brown rice affected pollen development, delayed flowering time, decreased fertility, and reduced grain yield under -Cu condition”
Claim · model-written summary
Mutation of OsWRKY37 affected pollen development under -Cu condition.
- Taxon
- RiceOryza sativat0
- Treatment
- mutation of OsWRKY37e3
- Outcome
- pollen developmente3
- Qualifier
- under -Cu conditione3
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice transcription factor OsWRKY37”
e3“While mutation of OsWRKY37 significantly decreased Cu concentrations in the stamen, the root-to-shoot translocation and distribution ratio in brown rice affected pollen development, delayed flowering time, decreased fertility, and reduced grain yield under -Cu condition”
Claim · model-written summary
Mutation of OsWRKY37 delayed flowering time under -Cu condition.
- Taxon
- RiceOryza sativat0
- Treatment
- mutation of OsWRKY37e3
- Outcome
- flowering timee3
- Direction
- delayede3
- Qualifier
- under -Cu conditione3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice transcription factor OsWRKY37”
e3“While mutation of OsWRKY37 significantly decreased Cu concentrations in the stamen, the root-to-shoot translocation and distribution ratio in brown rice affected pollen development, delayed flowering time, decreased fertility, and reduced grain yield under -Cu condition”
Claim · model-written summary
Mutation of OsWRKY37 decreased fertility under -Cu condition.
- Taxon
- RiceOryza sativat0
- Treatment
- mutation of OsWRKY37e3
- Outcome
- fertilitye3
- Direction
- decreasede3
- Qualifier
- under -Cu conditione3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice transcription factor OsWRKY37”
e3“While mutation of OsWRKY37 significantly decreased Cu concentrations in the stamen, the root-to-shoot translocation and distribution ratio in brown rice affected pollen development, delayed flowering time, decreased fertility, and reduced grain yield under -Cu condition”
Claim · model-written summary
Mutation of OsWRKY37 reduced grain yield under -Cu condition.
- Taxon
- RiceOryza sativat0
- Treatment
- mutation of OsWRKY37e3
- Outcome
- grain yielde3
- Direction
- reducede3
- Qualifier
- under -Cu conditione3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice transcription factor OsWRKY37”
e3“While mutation of OsWRKY37 significantly decreased Cu concentrations in the stamen, the root-to-shoot translocation and distribution ratio in brown rice affected pollen development, delayed flowering time, decreased fertility, and reduced grain yield under -Cu condition”
Claim · model-written summary
OsWRKY37 directly binds the upstream promoter region of OsCOPT6.
- Taxon
- RiceOryza sativat0
- Gene
- OsCOPT6e4
- Outcome
- directly bind to the upstream promoter regione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice transcription factor OsWRKY37”
e4“showed that OsWRKY37 could directly bind to the upstream promoter region of OsCOPT6 (copper transporter) and OsYSL16 (yellow stripe-like protein) and positively activate their expression levels”
Claim · model-written summary
OsWRKY37 positively activates the expression levels of OsCOPT6.
- Taxon
- RiceOryza sativat0
- Gene
- OsCOPT6e4
- Outcome
- expression levelse4
- Direction
- increaseinferrede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice transcription factor OsWRKY37”
e4“showed that OsWRKY37 could directly bind to the upstream promoter region of OsCOPT6 (copper transporter) and OsYSL16 (yellow stripe-like protein) and positively activate their expression levels”
Claim · model-written summary
OsWRKY37 directly binds the upstream promoter region of OsYSL16.
- Taxon
- RiceOryza sativat0
- Gene
- OsYSL16e4
- Outcome
- directly bind to the upstream promoter regione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice transcription factor OsWRKY37”
e4“showed that OsWRKY37 could directly bind to the upstream promoter region of OsCOPT6 (copper transporter) and OsYSL16 (yellow stripe-like protein) and positively activate their expression levels”
Claim · model-written summary
OsWRKY37 positively activates the expression levels of OsYSL16.
- Taxon
- RiceOryza sativat0
- Gene
- OsYSL16e4
- Outcome
- expression levelse4
- Direction
- increaseinferrede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice transcription factor OsWRKY37”
e4“showed that OsWRKY37 could directly bind to the upstream promoter region of OsCOPT6 (copper transporter) and OsYSL16 (yellow stripe-like protein) and positively activate their expression levels”
Claim · model-written summary
Analyses of oscopt6 mutants validated an important role of OsCOPT6 in copper uptake in rice.
- Taxon
- riceOryza sativae5
- Treatment
- oscopt6 mutantse5
- Gene
- oscopt6e5
- Outcome
- Cu uptakee5
- Direction
- decreaseinferrede5
- Qualifier
- important rolee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Analyses of oscopt6 mutants further validated its important role in Cu uptake in rice”
Claim · model-written summary
OsWRKY37 acts as a positive regulator of copper uptake, root-to-shoot translocation and distribution by activating OsCOPT6 and OsYSL16.
- Taxon
- RiceOryza sativat0
- Gene
- OsCOPT6 and OsYSL16e6
- Outcome
- uptake, root-to-shoot translocation, and distribution of Cue6
- Qualifier
- through activating the expression of OsCOPT6 and OsYSL16e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice transcription factor OsWRKY37”
e6“Our study demonstrated that OsWRKY37 acts as a positive regulator involved in the uptake, root-to-shoot translocation, and distribution of Cu through activating the expression of OsCOPT6 and OsYSL16”
Claim · model-written summary
A genetic strategy based on OsWRKY37 could improve rice yield under copper deficient conditions.
- Taxon
- riceOryza sativae7
- Outcome
- improving rice yielde7
- Qualifier
- under Cu deficient conditione7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Our results provide a genetic strategy for improving rice yield under Cu deficient condition”
The same questions in other papers
loss of function · phenology · Oryza sativa (rice)Positive and negative results
12 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
12 papers more in this group
See all 16 papers →loss of function · nutrient: content · Oryza sativa (rice)Positive and negative results
35 papers in the same direction as this paper · 20 in the opposite direction
- negative
- positive
- positive
52 papers more in this group
See all 56 papers →loss of function · growth · Oryza sativa (rice)Positive and negative results
103 papers in the same direction as this paper · 28 in the opposite direction
- positive
- positive
- positive
128 papers more in this group
See all 132 papers →loss of function · yield · Oryza sativa (rice)Positive and negative results
71 papers in the same direction as this paper · 62 in the opposite direction
- positive
- positive
- positive
130 papers more in this group
See all 134 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.