Abstract
Cytoplasmic male sterile (CMS) lines play a crucial role in utilization of heterosis in crop plants. However, the mechanism underlying the manipulation of male sterility in cotton by long non-coding RNA (lncRNA) and brassinosteroids (BRs) remains elusive. Here, using an integrative approach combining lncRNA transcriptomic profiles with virus-induced gene silencing experiments, we identify a flower bud-specific lncRNA in the maintainer line 2074B, lncRNA67, negatively modulating with male sterility in upland cotton (Gossypium hirsutum). lncRNA67 positively regulates cytochrome P274B (GhCYP724B), which acted as an eTM (endogenous target mimic) for miR3367. The suppression of GhCYP724B induced symptoms of BR deficiency and male semi-sterility in upland cotton as well as in tobacco, which resulted from a reduction in the endogenous BR contents. GhCYP724B regulates BRs synthesis by interacting with GhDIM and GhCYP90B, two BRs biosynthesis proteins. Additionally, GhCYP724B suppressed a unique chimeric open reading frame (Aorf27) in 2074A mitochondrial genome. Ectopic expression of Aorf27 in yeast inhibited cellular growth, and over expression of Aorf27 in tobacco showed male sterility. Overall, the results proved that the miR3367-lncRNA67-GhCYP724B module positively regulates male sterility by modulating BRs biosynthesis. The findings uncovered the function of lncRNA67-GhCYP724B in male sterility, providing a new mechanism for understanding male sterility in upland cotton.
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Claims 11
Claim · model-written summary
Flower bud-specific lncRNA67 negatively modulated male sterility in upland cotton.
- Taxon
- Gossypium hirsutum
Gossypium hirsutume0 - Material
- 2074Be0
- Organ / stage
- flower bude0
- Gene
- lncRNA67e0
- Outcome
- male sterilitye0
- Direction
- negativelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identify a flower bud-specific lncRNA in the maintainer line 2074B, lncRNA67, negatively modulating with male sterility in upland cotton (Gossypium hirsutum).”
Claim · model-written summary
lncRNA67 positively regulated GhCYP724B.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Gene
- lncRNA67e0
- Outcome
- positively regulates cytochrome P274B (GhCYP724B)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lncRNA67 positively regulates cytochrome P274B (GhCYP724B), which acted as an eTM (endogenous target mimic) for miR3367.”
t0“Gossypium hirsutum”
Claim · model-written summary
GhCYP724B acted as an endogenous target mimic for miR3367.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Gene
- GhCYP724Be0
- Outcome
- acted as an eTM (endogenous target mimic) for miR3367e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lncRNA67 positively regulates cytochrome P274B (GhCYP724B), which acted as an eTM (endogenous target mimic) for miR3367.”
t0“Gossypium hirsutum”
Claim · model-written summary
Suppressing GhCYP724B induced brassinosteroid deficiency and male semi-sterility in cotton.
- Taxon
- upland cottone0
- Treatment
- suppression of GhCYP724Be0
- Gene
- GhCYP724Be0
- Outcome
- symptoms of BR deficiency and male semi-sterilitye0
Not reportedComparatorDirection
Verbatim
e0“The suppression of GhCYP724B induced symptoms of BR deficiency and male semi-sterility in upland cotton as well as in tobacco, which resulted from a reduction in the endogenous BR contents.”
Claim · model-written summary
Suppressing GhCYP724B induced brassinosteroid deficiency and male semi-sterility in tobacco.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- suppression of GhCYP724Be0
- Gene
- GhCYP724Be0
- Outcome
- symptoms of BR deficiency and male semi-sterilitye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The suppression of GhCYP724B induced symptoms of BR deficiency and male semi-sterility in upland cotton as well as in tobacco, which resulted from a reduction in the endogenous BR contents.”
Claim · model-written summary
GhCYP724B suppression reduced endogenous BR content.
- Taxon
- upland cottone0
- Treatment
- suppression of GhCYP724Be0
- Gene
- GhCYP724Be0
- Outcome
- endogenous BR contentse0
- Direction
- reductione0
Not reportedComparator
Verbatim
e0“The suppression of GhCYP724B induced symptoms of BR deficiency and male semi-sterility in upland cotton as well as in tobacco, which resulted from a reduction in the endogenous BR contents.”
Claim · model-written summary
GhCYP724B interacted with GhDIM and GhCYP90B to regulate BR synthesis.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Gene
- GhCYP724Be0
- Outcome
- BRs synthesise0
- Qualifier
- interacting with GhDIM and GhCYP90Be0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhCYP724B regulates BRs synthesis by interacting with GhDIM and GhCYP90B, two BRs biosynthesis proteins.”
t0“Gossypium hirsutum”
Claim · model-written summary
GhCYP724B suppressed Aorf27 in the 2074A mitochondrial genome.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Material
- 2074Ae0
- Gene
- GhCYP724Be0
- Outcome
- suppressed a unique chimeric open reading frame (Aorf27)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhCYP724B suppressed a unique chimeric open reading frame (Aorf27) in 2074A mitochondrial genome.”
t0“Gossypium hirsutum”
Claim · model-written summary
Ectopic Aorf27 expression inhibited yeast cellular growth.
- Taxon
- yeaste0
- Treatment
- Ectopic expression of Aorf27e0
- Gene
- Aorf27e0
- Outcome
- cellular growthe0
- Direction
- inhibitede0
Not reportedComparator
Verbatim
e0“Ectopic expression of Aorf27 in yeast inhibited cellular growth, and over expression of Aorf27 in tobacco showed male sterility.”
Claim · model-written summary
Aorf27 overexpression caused male sterility in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- over expression of Aorf27e0
- Gene
- Aorf27e0
- Outcome
- male sterilitye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of Aorf27 in yeast inhibited cellular growth, and over expression of Aorf27 in tobacco showed male sterility.”
Claim · model-written summary
The miR3367-lncRNA67-GhCYP724B module positively regulated male sterility through BR biosynthesis.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Gene
- miR3367-lncRNA67-GhCYP724Be0
- Outcome
- male sterilitye0
- Qualifier
- modulating BRs biosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the miR3367-lncRNA67-GhCYP724B module positively regulates male sterility by modulating BRs biosynthesis.”
t0“Gossypium hirsutum”
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