Paper · 2025 · Applied Food Research

Lemon verbena nanoemulsion: A natural solution for improving quality and shelf life of Thomson Navel orange (Citrus sinensis L.)

Houshmand Sharafi; Elham Rezaei; Nashmil Farhadi; et al.

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Open access · cc-by-nc-nd

Abstract

• A lemon verbena essential oil nanoemulsion was prepared using the phase-inversion method. • Nanoemulsions have been used to improve the postharvest quality of Thamson fruits. • NL coating, especially at 2 %, positively affected fruit quality. • Nanoemulsions are natural preservatives for the postharvest storage of fruits. The aim of the current study was to evaluate the effect of Lemon verbena essential oil (LVEO) nanoemulsion coating on the postharvest quality of Thomson Navel orange ( Citrus sinensis cv. Thomson Navel). Nanoemulsions were prepared using the emulsion phase-inversion method. The effects of nanoemulsion (NL) solutions containing 0.25 % (NL 0.25 ), 0.50 % (NL 0.50 ), 0.75 % (NL 0.75 ), 1.00 % (NL 1.00 ), 1.50 % (NL 1.50 ), and 2.00 % (NL 2.00 ) of LVEO on Thomson Navel fruits were investigated during a 100-day storage under cold conditions. The following results are the notable impact of coating treatments on the phytochemical and antioxidant properties of the Thomson fruit at the end of storage. NL 2.00 coated fruits had the lowest pH (3.90 ± 0.03), and highest titratable acidity (0.79 ± 0.05 %), and vitamin C content [45.4 ± 1.6 mg ascorbic acid/100 g fresh weight (FW)] compared to the other treatments. Coated fruits with NL 0.75 had the best effect on tannins and carotenoid content, with 1.7 ± 0.1 mg tannic acid equivalent/mL Juice and 227 ± 7 mg/100 mL Juice concentration, respectively. NL 0.50 -coated fruits showed the highest antioxidant capacity (48.39 %) and total phenolic content [11.6 ± 0.3 mg gallic acid equivalent/100 g fresh weight]. The control group showed higher total soluble solids (16 ± 0.0 %), and NL 1.00 treatment was the most beneficial for preserving total flavonoid content (9.1 ± 0.6 mg quercetin equivalent/100 g fresh weight) in coated fruits. Principal component analysis outputs were consistent with our results, indicating that the NL 1.00 treatment exhibited the highest total flavonoid content. The hierarchical cluster analysis on day 100 showed fruits treated with NL 0.25 and NL 0.75 had similar phytochemical and antioxidant parameters to those of the control during the 20-day storage period. The results of this study indicate the potential of LVEO nanoemulsion as a natural preservative for the postharvest storage of fruits.

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Claims 11

Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

NL 2.00 coated oranges had the lowest pH, 3.90 ± 0.03.

Taxon
oranget0
Treatment
NL 2.00e0
Outcome
pHe0
Direction
loweste0
Value
3.90 ± 0.03e0
Comparator
other treatmentse0

Verbatim

e0

“NL 2.00 coated fruits had the lowest pH (3.90 ± 0.03), and highest titratable acidity (0.79 ± 0.05 %), and vitamin C content [45.4 ± 1.6 mg ascorbic acid/100 g fresh weight (FW)] compared to the other treatments”

✓ located verbatim in the source · abstract, characters 1029–1240 · show in text ↑
t0

“orange”

✓ located verbatim in the source · title, characters 101–107 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

NL 2.00 coated oranges had the highest titratable acidity, 0.79 ± 0.05 %.

Taxon
oranget0
Treatment
NL 2.00e0
Outcome
titratable aciditye0
Direction
higheste0
Value
0.79 ± 0.05 %e0
Comparator
other treatmentse0

Verbatim

e0

“NL 2.00 coated fruits had the lowest pH (3.90 ± 0.03), and highest titratable acidity (0.79 ± 0.05 %), and vitamin C content [45.4 ± 1.6 mg ascorbic acid/100 g fresh weight (FW)] compared to the other treatments”

✓ located verbatim in the source · abstract, characters 1029–1240 · show in text ↑
t0

“orange”

✓ located verbatim in the source · title, characters 101–107 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

NL 2.00 coated oranges had the highest vitamin C content, 45.4 ± 1.6 mg per 100 g fresh weight.

Taxon
oranget0
Treatment
NL 2.00e0
Outcome
vitamin C contente0
Direction
higheste0
Value
45.4 ± 1.6 mg ascorbic acid/100 g fresh weighte0
Comparator
other treatmentse0

Verbatim

e0

“NL 2.00 coated fruits had the lowest pH (3.90 ± 0.03), and highest titratable acidity (0.79 ± 0.05 %), and vitamin C content [45.4 ± 1.6 mg ascorbic acid/100 g fresh weight (FW)] compared to the other treatments”

✓ located verbatim in the source · abstract, characters 1029–1240 · show in text ↑
t0

“orange”

✓ located verbatim in the source · title, characters 101–107 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

NL 0.75 coated oranges had the best tannin content, 1.7 ± 0.1 mg tannic acid equivalent/mL Juice.

Taxon
oranget0
Treatment
NL 0.75e0
Outcome
tanninse0
Direction
beste0
Value
1.7 ± 0.1 mg tannic acid equivalent/mL Juicee0

Not reportedComparator

Verbatim

e0

“Coated fruits with NL 0.75 had the best effect on tannins and carotenoid content, with 1.7 ± 0.1 mg tannic acid equivalent/mL Juice and 227 ± 7 mg/100 mL Juice concentration, respectively”

✓ located verbatim in the source · abstract, characters 1242–1429 · show in text ↑
t0

“orange”

✓ located verbatim in the source · title, characters 101–107 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

NL 0.75 coated oranges had the best carotenoid content, 227 ± 7 mg/100 mL Juice.

Taxon
oranget0
Treatment
NL 0.75e0
Outcome
carotenoid contente0
Direction
beste0
Value
227 ± 7 mg/100 mL Juicee0

Not reportedComparator

Verbatim

e0

“Coated fruits with NL 0.75 had the best effect on tannins and carotenoid content, with 1.7 ± 0.1 mg tannic acid equivalent/mL Juice and 227 ± 7 mg/100 mL Juice concentration, respectively”

✓ located verbatim in the source · abstract, characters 1242–1429 · show in text ↑
t0

“orange”

✓ located verbatim in the source · title, characters 101–107 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

NL 0.50 coated oranges had the highest antioxidant capacity, 48.39 %.

Taxon
oranget0
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NL 0.50e0
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antioxidant capacitye0
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higheste0
Value
48.39 %e0

Not reportedComparator

Verbatim

e0

“NL 0.50 -coated fruits showed the highest antioxidant capacity (48.39 %) and total phenolic content [11.6 ± 0.3 mg gallic acid equivalent/100 g fresh weight]”

✓ located verbatim in the source · abstract, characters 1431–1588 · show in text ↑
t0

“orange”

✓ located verbatim in the source · title, characters 101–107 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

NL 0.50 coated oranges had the highest total phenolic content, 11.6 ± 0.3 mg gallic acid equivalent/100 g fresh weight.

Taxon
oranget0
Treatment
NL 0.50e0
Outcome
total phenolic contente0
Direction
higheste0
Value
11.6 ± 0.3 mg gallic acid equivalent/100 g fresh weighte0

Not reportedComparator

Verbatim

e0

“NL 0.50 -coated fruits showed the highest antioxidant capacity (48.39 %) and total phenolic content [11.6 ± 0.3 mg gallic acid equivalent/100 g fresh weight]”

✓ located verbatim in the source · abstract, characters 1431–1588 · show in text ↑
t0

“orange”

✓ located verbatim in the source · title, characters 101–107 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Control oranges had higher total soluble solids, 16 ± 0.0 %.

Taxon
oranget0
Outcome
total soluble solidse0
Direction
highere0
Value
16 ± 0.0 %e0

Verbatim

e0

“The control group showed higher total soluble solids (16 ± 0.0 %)”

✓ located verbatim in the source · abstract, characters 1590–1655 · show in text ↑
t0

“orange”

✓ located verbatim in the source · title, characters 101–107 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

NL 1.00 best preserved total flavonoids, 9.1 ± 0.6 mg quercetin equivalent/100 g fresh weight.

Taxon
oranget0
Treatment
NL 1.00e0
Outcome
total flavonoid contente0
Direction
most beneficiale0
Value
9.1 ± 0.6 mg quercetin equivalent/100 g fresh weighte0

Not reportedComparator

Verbatim

e0

“NL 1.00 treatment was the most beneficial for preserving total flavonoid content (9.1 ± 0.6 mg quercetin equivalent/100 g fresh weight) in coated fruits”

✓ located verbatim in the source · abstract, characters 1661–1813 · show in text ↑
t0

“orange”

✓ located verbatim in the source · title, characters 101–107 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

At day 100, NL 0.25 fruit phytochemical and antioxidant parameters resembled those of controls during the 20-day storage period.

Taxon
oranget0
Treatment
NL 0.25e0
Outcome
phytochemical and antioxidant parameterse0
Direction
similare0
Comparator
controle0

Verbatim

e0

“The hierarchical cluster analysis on day 100 showed fruits treated with NL 0.25 and NL 0.75 had similar phytochemical and antioxidant parameters to those of the control during the 20-day storage period”

✓ located verbatim in the source · abstract, characters 1971–2172 · show in text ↑
t0

“orange”

✓ located verbatim in the source · title, characters 101–107 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

At day 100, NL 0.75 fruit phytochemical and antioxidant parameters resembled those of controls during the 20-day storage period.

Taxon
oranget0
Treatment
NL 0.75e0
Outcome
phytochemical and antioxidant parameterse0
Direction
similare0
Comparator
controle0

Verbatim

e0

“The hierarchical cluster analysis on day 100 showed fruits treated with NL 0.25 and NL 0.75 had similar phytochemical and antioxidant parameters to those of the control during the 20-day storage period”

✓ located verbatim in the source · abstract, characters 1971–2172 · show in text ↑
t0

“orange”

✓ located verbatim in the source · title, characters 101–107 · show in text ↑

nanoparticle · quality: sugar starch · Citrus sinensis (sweet orange)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
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nanoparticle · quality: phytochemicals · Citrus sinensis (sweet orange)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
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nanoparticle · physiology: pigments · Citrus sinensis (sweet orange)Results agree

5 papers: 5 positive, 0 negative or no effect

4 papers in the same direction as this paper

  1. positive
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.