Abstract
Drought, the most unfavorable and widespread environmental factor among abiotic stresses, has become a significant global issue that severely crop growth and yield. Nuclear factor-Y (NF-Y) transcription factors have well-documented functions in regulating stress tolerance in plants. However, the specific functions and molecular mechanisms of NF-YS in conferring drought tolerance in apples remain largely unexplored. Here, we reported that the NF-Y transcription factor MhNF-YA1-like positively modulates drought stress tolerance in apple (Malus domestica Borkh.). MhNF-YA1-like is transcriptionally upregulated in response to drought and abscisic acid (ABA) treatments. Overexpression of MhNF-YA1-like enhances drought tolerance in apples, whereas its silencing results in weakened drought tolerance. Further investigation reveals that MhNF-YA1-like directly binds to the promoter of MhAAO3 and activates its expression. Additionally, we identified MhDnaJC76 as an interacting protein of MhNF-YA1, and MhDnaJC76 positively modulates apple drought tolerance. Furthermore, the interaction between MhNF-YA1-like and MhDnaJC76 significantly amplifies the regulatory effect of MhNF-YA1-like on MhAAO3, thereby promoting ABA accumulation and consequently enhancing apple drought tolerance. Taken together, our findings elucidate a molecular framework by which the MhNF-YA1-like-MhDnaJC76 complex modulates drought tolerance in apples, thereby providing targets for developing drought-tolerant crops.
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Claims 8
Claim · model-written summary
MhNF-YA1-like expression increased after drought and ABA treatments.
- Taxon
- apple
Malus domesticae1 - Treatment
- drought and abscisic acid (ABA) treatmentse0
- Gene
- MhNF-YA1-likee0
- Outcome
- MhNF-YA1-likee0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MhNF-YA1-like is transcriptionally upregulated in response to drought and abscisic acid (ABA) treatments.”
e1“apple (Malus domestica Borkh.)”
Claim · model-written summary
Overexpression of MhNF-YA1-like enhanced apple drought tolerance.
- Taxon
- appleMalus domesticae1
- Treatment
- Overexpression of MhNF-YA1-likee0
- Gene
- MhNF-YA1-likee0
- Outcome
- drought tolerancee0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of MhNF-YA1-like enhances drought tolerance in apples”
e1“apple (Malus domestica Borkh.)”
Claim · model-written summary
Silencing MhNF-YA1-like weakened apple drought tolerance.
- Taxon
- appleMalus domesticae1
- Treatment
- silencinge0
- Gene
- MhNF-YA1-likee2
- Outcome
- drought tolerancee0
- Direction
- weakenede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its silencing results in weakened drought tolerance”
e1“apple (Malus domestica Borkh.)”
e2“MhNF-YA1-like directly binds”
Claim · model-written summary
MhNF-YA1-like bound the MhAAO3 promoter and activated its expression.
- Taxon
- appleMalus domesticae1
- Gene
- MhAAO3e0
- Outcome
- its expressione0
- Direction
- activatese0
- Qualifier
- MhNF-YA1-like directly binds to the promotere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MhNF-YA1-like directly binds to the promoter of MhAAO3 and activates its expression”
e1“apple (Malus domestica Borkh.)”
Claim · model-written summary
MhDnaJC76 was identified as an interacting protein of MhNF-YA1.
- Taxon
- appleMalus domesticae1
- Gene
- MhDnaJC76e0
- Outcome
- interacting protein of MhNF-YA1e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified MhDnaJC76 as an interacting protein of MhNF-YA1”
e1“apple (Malus domestica Borkh.)”
Claim · model-written summary
MhDnaJC76 positively regulated apple drought tolerance.
- Taxon
- appleMalus domesticae0
- Gene
- MhDnaJC76e0
- Outcome
- drought tolerancee0
- Direction
- positivelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MhDnaJC76 positively modulates apple drought tolerance”
Claim · model-written summary
The MhNF-YA1-like–MhDnaJC76 interaction amplified regulation of MhAAO3.
- Taxon
- appleMalus domesticae1
- Gene
- MhAAO3e0
- Outcome
- regulatory effect of MhNF-YA1-like on MhAAO3e0
- Direction
- amplifiese0
- Qualifier
- interaction between MhNF-YA1-like and MhDnaJC76e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the interaction between MhNF-YA1-like and MhDnaJC76 significantly amplifies the regulatory effect of MhNF-YA1-like on MhAAO3”
e1“apple (Malus domestica Borkh.)”
Claim · model-written summary
The interaction promoted ABA accumulation and apple drought tolerance.
- Taxon
- appleMalus domesticae0
- Compound
- ABAe0
- Outcome
- ABA accumulation and consequently enhancing apple drought tolerancee0
- Direction
- promotinge0
- Qualifier
- interaction between MhNF-YA1-like and MhDnaJC76e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“thereby promoting ABA accumulation and consequently enhancing apple drought tolerance”
e1“interaction between MhNF-YA1-like and MhDnaJC76”
The same questions in other papers
loss of function · tolerance: abiotic · Malus domestica (apple)Positive and negative results
2 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.