Paper · 2025 · Scientific Reports

Bio and chemical fertilizers integration supplemented by biostimulants a sustainable way to reduce chemical inputs in rainfed wheat systems

Layegh Moradi; Adel Siosemardeh

18
claims
46
verbatim quotes
3
citations
2
research areas
✓
Open access · cc-by-nc-nd

Abstract

A sustainable solution to reduce chemical inputs in rainfed wheat systems is to replace part of the chemical fertilizers with biofertilizers, combined with foliar application. During 2023–2024, a field experiment was conducted at two sites at research farms in Javanroud and Dehgolan, Iran. Five integrated fertilizer levels [100% chemical fertilizer (CF100), 75% CF + biofertilizers (CF75 + BF), 50% CF + BF (CF50 + BF), 25% CF + BF (CF25 + BF), and 0% CF + BF (CF0 + BF)] with three foliar applications (control, seaweed, and amino acids) were tested. A complementary pot experiment was conducted to assess treatment effects on root growth parameters specifically. Overall, in comparison to other treatments, CF75 + BF and CF100 improved root growth, physiological attributes, and grain yield and quality parameters. In all fertilizer integration strategies, foliar application of seaweed extracts and amino acids improved plant performance indicators. The CF75 + BF treatment, supplemented with seaweed foliar application, achieved maximum chlorophyll levels (2.35 mg g ─ 1 FW), membrane stability index (82.69%), relative water content (84.50%), peroxidase activity (0.307 unit mg ─ 1 protein), biological yield (10,183.7 kg ha ─ 1 ), grain yield (3,343.4 kg ha ─ 1 ), and grain Zn content (36.43 g g ─ 1 ), as well as minimum malondialdehyde (1.72 g g ─ 1 FW) and H 2 O 2 (141.4 nmol g ─1 FW). Furthermore, the greatest root length (91.90 cm), root dry mass (4.86 g pot ─ 1 ), grain Fe content (43.34 µg g ─ 1 ), and grain phosphorus content (3.27%) were obtained for CF75 + BF. Based on the results, a 25% reduced chemical fertilizer dose, combined with biofertilizers and foliar seaweed extract application, appears to be the optimal approach.

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Claims 18

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

CF75 + BF improved root growth, physiology, grain yield and quality relative to other treatments.

Treatment
CF75 + BFe0
Outcome
root growth, physiological attributes, and grain yield and quality parameterse0
Direction
improvede0
Comparator
other treatmentse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“in comparison to other treatments, CF75 + BF and CF100 improved root growth, physiological attributes, and grain yield and quality parameters.”

✓ located verbatim in the source · abstract, characters 676–818 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 126–131 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

CF100 improved root growth, physiology, grain yield and quality relative to other treatments.

Treatment
CF100e0
Outcome
root growth, physiological attributes, and grain yield and quality parameterse0
Direction
improvede0
Comparator
other treatmentse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“in comparison to other treatments, CF75 + BF and CF100 improved root growth, physiological attributes, and grain yield and quality parameters.”

✓ located verbatim in the source · abstract, characters 676–818 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 126–131 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Seaweed foliar application improved plant performance indicators across fertilizer strategies.

Treatment
foliar application of seaweed extractse0
Outcome
plant performance indicatorse0
Direction
improvede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“In all fertilizer integration strategies, foliar application of seaweed extracts and amino acids improved plant performance indicators.”

✓ located verbatim in the source · abstract, characters 819–954 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 126–131 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Amino acid foliar application improved plant performance indicators across fertilizer strategies.

Treatment
amino acidse0
Outcome
plant performance indicatorse0
Direction
improvede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“In all fertilizer integration strategies, foliar application of seaweed extracts and amino acids improved plant performance indicators.”

✓ located verbatim in the source · abstract, characters 819–954 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 126–131 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

CF75 + BF with seaweed produced maximum chlorophyll of 2.35 mg g ─ 1 FW.

Treatment
CF75 + BF treatment, supplemented with seaweed foliar applicatione0
Outcome
chlorophyll levelse0
Direction
maximume0
Value
2.35 mg g ─ 1 FWe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The CF75 + BF treatment, supplemented with seaweed foliar application, achieved maximum chlorophyll levels (2.35 mg g ─ 1 FW)”

✓ located verbatim in the source · abstract, characters 955–1080 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 126–131 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

CF75 + BF with seaweed produced maximum membrane stability index of 82.69%.

Treatment
seaweed foliar applicatione1
Outcome
membrane stability indexe0
Direction
maximume0
Value
82.69%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“maximum chlorophyll levels (2.35 mg g ─ 1 FW), membrane stability index (82.69%), relative water content (84.50%)”

✓ located verbatim in the source · abstract, characters 1035–1148 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 126–131 · show in text ↑
e1

“The CF75 + BF treatment, supplemented with seaweed foliar application, achieved maximum chlorophyll levels”

✓ located verbatim in the source · abstract, characters 955–1061 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

CF75 + BF with seaweed produced maximum relative water content of 84.50%.

Treatment
seaweed foliar applicatione1
Outcome
relative water contente0
Direction
maximume0
Value
84.50%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“maximum chlorophyll levels (2.35 mg g ─ 1 FW), membrane stability index (82.69%), relative water content (84.50%)”

✓ located verbatim in the source · abstract, characters 1035–1148 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 126–131 · show in text ↑
e1

“The CF75 + BF treatment, supplemented with seaweed foliar application, achieved maximum chlorophyll levels”

✓ located verbatim in the source · abstract, characters 955–1061 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

CF75 + BF with seaweed produced maximum peroxidase activity of 0.307 unit mg ─ 1 protein.

Treatment
seaweed foliar applicatione1
Outcome
peroxidase activitye0
Direction
increaseinferrede0
Value
0.307 unit mg ─ 1 proteine0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“peroxidase activity (0.307 unit mg ─ 1 protein), biological yield (10,183.7 kg ha ─ 1 )”

✓ located verbatim in the source · abstract, characters 1150–1237 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 126–131 · show in text ↑
e1

“The CF75 + BF treatment, supplemented with seaweed foliar application, achieved maximum chlorophyll levels”

✓ located verbatim in the source · abstract, characters 955–1061 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

CF75 + BF with seaweed produced maximum biological yield of 10,183.7 kg ha ─ 1.

Treatment
seaweed foliar applicatione1
Outcome
biological yielde0
Direction
increaseinferrede0
Value
10,183.7 kg ha ─ 1e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“biological yield (10,183.7 kg ha ─ 1 ), grain yield (3,343.4 kg ha ─ 1 )”

✓ located verbatim in the source · abstract, characters 1199–1271 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 126–131 · show in text ↑
e1

“The CF75 + BF treatment, supplemented with seaweed foliar application, achieved maximum chlorophyll levels”

✓ located verbatim in the source · abstract, characters 955–1061 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

CF75 + BF with seaweed produced maximum grain yield of 3,343.4 kg ha ─ 1.

Treatment
seaweed foliar applicatione1
Outcome
grain yielde0
Direction
increaseinferrede0
Value
3,343.4 kg ha ─ 1e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“grain yield (3,343.4 kg ha ─ 1 ), and grain Zn content (36.43 g g ─ 1 )”

✓ located verbatim in the source · abstract, characters 1239–1310 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 126–131 · show in text ↑
e1

“The CF75 + BF treatment, supplemented with seaweed foliar application, achieved maximum chlorophyll levels”

✓ located verbatim in the source · abstract, characters 955–1061 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

CF75 + BF with seaweed produced maximum grain zinc content of 36.43 g g ─ 1.

Treatment
seaweed foliar applicatione1
Outcome
grain Zn contente0
Direction
increaseinferrede0
Value
36.43 g g ─ 1e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“grain Zn content (36.43 g g ─ 1 ), as well as minimum malondialdehyde (1.72 g g ─ 1 FW)”

✓ located verbatim in the source · abstract, characters 1277–1364 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 126–131 · show in text ↑
e1

“The CF75 + BF treatment, supplemented with seaweed foliar application, achieved maximum chlorophyll levels”

✓ located verbatim in the source · abstract, characters 955–1061 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

CF75 + BF with seaweed produced minimum malondialdehyde of 1.72 g g ─ 1 FW.

Treatment
seaweed foliar applicatione1
Outcome
malondialdehydee0
Direction
minimume0
Value
1.72 g g ─ 1 FWe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“minimum malondialdehyde (1.72 g g ─ 1 FW) and H 2 O 2 (141.4 nmol g ─1 FW)”

✓ located verbatim in the source · abstract, characters 1323–1397 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 126–131 · show in text ↑
e1

“The CF75 + BF treatment, supplemented with seaweed foliar application, achieved maximum chlorophyll levels”

✓ located verbatim in the source · abstract, characters 955–1061 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

CF75 + BF with seaweed produced minimum hydrogen peroxide of 141.4 nmol g ─1 FW.

Treatment
seaweed foliar applicatione1
Outcome
H 2 O 2e0
Direction
minimume0
Value
141.4 nmol g ─1 FWe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“minimum malondialdehyde (1.72 g g ─ 1 FW) and H 2 O 2 (141.4 nmol g ─1 FW)”

✓ located verbatim in the source · abstract, characters 1323–1397 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 126–131 · show in text ↑
e1

“The CF75 + BF treatment, supplemented with seaweed foliar application, achieved maximum chlorophyll levels”

✓ located verbatim in the source · abstract, characters 955–1061 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

CF75 + BF gave greatest root length of 91.90 cm.

Treatment
CF75 + BFe1
Outcome
root lengthe0
Direction
greateste0
Value
91.90 cme0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the greatest root length (91.90 cm), root dry mass (4.86 g pot ─ 1 )”

✓ located verbatim in the source · abstract, characters 1412–1480 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 126–131 · show in text ↑
e1

“The CF75 + BF treatment, supplemented with seaweed foliar application, achieved maximum chlorophyll levels”

✓ located verbatim in the source · abstract, characters 955–1061 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

CF75 + BF gave greatest root dry mass of 4.86 g pot ─ 1.

Treatment
CF75 + BFe1
Outcome
root dry masse0
Direction
greateste0
Value
4.86 g pot ─ 1e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the greatest root length (91.90 cm), root dry mass (4.86 g pot ─ 1 )”

✓ located verbatim in the source · abstract, characters 1412–1480 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 126–131 · show in text ↑
e1

“The CF75 + BF treatment, supplemented with seaweed foliar application, achieved maximum chlorophyll levels”

✓ located verbatim in the source · abstract, characters 955–1061 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

CF75 + BF gave greatest grain iron content of 43.34 µg g ─ 1.

Treatment
CF75 + BFe0
Outcome
grain Fe contente0
Direction
higherinferrede0
Value
43.34 µg g ─ 1e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“grain Fe content (43.34 µg g ─ 1 ), and grain phosphorus content (3.27%) were obtained for CF75 + BF.”

✓ located verbatim in the source · abstract, characters 1482–1583 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 126–131 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

CF75 + BF gave greatest grain phosphorus content of 3.27%.

Treatment
CF75 + BFe0
Outcome
grain phosphorus contente0
Direction
higherinferrede0
Value
3.27%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“grain Fe content (43.34 µg g ─ 1 ), and grain phosphorus content (3.27%) were obtained for CF75 + BF.”

✓ located verbatim in the source · abstract, characters 1482–1583 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 126–131 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors recommend a 25% chemical fertilizer reduction with biofertilizers and foliar seaweed.

Outcome
25% reduced chemical fertilizer dose, combined with biofertilizers and foliar seaweed extract applicatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“a 25% reduced chemical fertilizer dose, combined with biofertilizers and foliar seaweed extract application, appears to be the optimal approach.”

✓ located verbatim in the source · abstract, characters 1606–1750 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 126–131 · show in text ↑

other · physiology: water · Triticum aestivum (wheat)Positive and negative results

17 papers: 16 positive, 1 negative or no effect

15 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. positive

13 papers more in this group

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application · physiology: oxidative · Triticum aestivum (wheat)Positive and negative results

45 papers: 44 positive, 1 negative or no effect

43 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. positive

41 papers more in this group

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application · physiology: water · Triticum aestivum (wheat)Positive and negative results

51 papers: 49 positive, 2 negative or no effect

48 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. negative
  3. positive

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application · physiology: antioxidant enzymes · Triticum aestivum (wheat)Positive and negative results

53 papers: 52 positive, 1 negative or no effect

51 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. positive

49 papers more in this group

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other · nutrient: content · Triticum aestivum (wheat)Positive and negative results

75 papers: 64 positive, 11 negative or no effect

63 papers in the same direction as this paper · 11 in the opposite direction

  1. no effect
  2. no effect
  3. no effect

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application · physiology: pigments · Triticum aestivum (wheat)Positive and negative results

106 papers: 98 positive, 8 negative or no effect

97 papers in the same direction as this paper · 6 in the opposite direction · 2 reporting no difference from an alternative

  1. positive
  2. negative
  3. negative

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other · yield · Triticum aestivum (wheat)Positive and negative results

223 papers: 206 positive, 17 negative or no effect

205 papers in the same direction as this paper · 14 in the opposite direction · 3 reporting no difference from an alternative

  1. positive
  2. positive
  3. no effect

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application · nutrient: content · Triticum aestivum (wheat)Positive and negative results

275 papers: 257 positive, 18 negative or no effect

256 papers in the same direction as this paper · 17 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. no effect
  3. no effect

271 papers more in this group

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8 of 12 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.