Abstract
Abstract Fusarium head blight caused by Fusarium graminearum is a serious disease that affects cereal crops worldwide, leading to significant yield losses. The Gβ-like protein Cpc2 has been documented as a key regulator of growth, development, and virulence in pathogenic fungi such as Cryptococcus neoformans and Aspergillus fumigatus . However, the specific biological functions of its ortholog, FgCpc2, in F. graminearum remain unexplored. Phylogenetic analysis reveals that FgCpc2 is highly conserved across various species and shares notable amino acid sequence similarity with Gβ-like proteins. Using the gene deletion approach, we found that FgCpc2 is critical for vegetative growth, conidiation, and sexual development in F. graminearum . The Δ Fgcpc2 mutant showed increased sensitivity to a range of stress factors, including osmotic, oxidative, and cell wall-disrupting agents. Furthermore, FgCpc2 is central to the pathogenicity of F. graminearum , as the Δ Fgcpc2 mutant exhibits reduced disease symptoms on wheat coleoptiles and flowering heads. Importantly, FgCpc2 is required for deoxynivalenol (DON) production, as the Δ FgCpc2 mutants produce significantly fewer toxisomes compared to the wild type. Transcriptomic analysis showed that FgCpc2 affects mycelial growth by regulating amino acid metabolism, the biosynthesis of secondary metabolites ( TRI gene family) and ABC transporters. Subcellular localization experiments revealed that FgCpc2 is uniformly distributed in the cytoplasm throughout different stages of fungal development. Overall, these findings highlight the crucial roles of FgCpc2 and lay a foundation for developing targeted strategies against Fusarium head blight.
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Claims 8
Claim · model-written summary
FgCpc2 is critical for vegetative growth, conidiation and sexual development in F. graminearum.
- Taxon
- wheat
Triticum aestivume2 - Interactor
- F. graminearum
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearume1pathogen - Gene
- FgCpc2e1
- Outcome
- vegetative growth, conidiation, and sexual developmente1
- Qualifier
- criticale1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
e1“we found that FgCpc2 is critical for vegetative growth, conidiation, and sexual development in F. graminearum”
e2“reduced disease symptoms on wheat coleoptiles and flowering heads”
Claim · model-written summary
The Δ Fgcpc2 mutant showed increased sensitivity to osmotic, oxidative and cell wall-disrupting agents.
- Taxon
- wheatTriticum aestivume3
- Material
- Δ Fgcpc2 mutante1
- Treatment
- osmotic, oxidative, and cell wall-disrupting agentse2
- Outcome
- sensitivity to a range of stress factorse1
- Direction
- increasede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The Δ Fgcpc2 mutant showed increased sensitivity to a range of stress factors”
e2“including osmotic, oxidative, and cell wall-disrupting agents”
e3“reduced disease symptoms on wheat coleoptiles and flowering heads”
Claim · model-written summary
The Δ Fgcpc2 mutant reduced disease symptoms on wheat coleoptiles and flowering heads.
- Taxon
- wheatTriticum aestivume1
- Organ / stage
- coleoptiles and flowering headse1
- Treatment
- Δ Fgcpc2 mutante1
- Outcome
- disease symptomse1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the Δ Fgcpc2 mutant exhibits reduced disease symptoms on wheat coleoptiles and flowering heads”
e2“FgCpc2 is central to the pathogenicity of F. graminearum”
Claim · model-written summary
FgCpc2 is required for deoxynivalenol (DON) production.
- Taxon
- wheatTriticum aestivume2
- Gene
- FgCpc2e1
- Outcome
- deoxynivalenol (DON) productione1
- Qualifier
- requirede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Importantly, FgCpc2 is required for deoxynivalenol (DON) production”
e2“reduced disease symptoms on wheat coleoptiles and flowering heads”
Claim · model-written summary
Δ FgCpc2 mutants produced significantly fewer toxisomes than the wild type.
- Taxon
- wheatTriticum aestivume2
- Treatment
- Δ FgCpc2 mutantse1
- Outcome
- toxisomese1
- Direction
- fewere1
- Comparator
- wild typee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the Δ FgCpc2 mutants produce significantly fewer toxisomes compared to the wild type”
e2“reduced disease symptoms on wheat coleoptiles and flowering heads”
Claim · model-written summary
FgCpc2 is suggested to affect mycelial growth by regulating amino acid metabolism.
- Taxon
- wheatTriticum aestivume2
- Gene
- FgCpc2e1
- Outcome
- mycelial growthe1
- Qualifier
- regulating amino acid metabolisme1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Transcriptomic analysis showed that FgCpc2 affects mycelial growth by regulating amino acid metabolism, the biosynthesis of secondary metabolites ( TRI gene family) and ABC transporters”
e2“reduced disease symptoms on wheat coleoptiles and flowering heads”
Claim · model-written summary
FgCpc2 is uniformly distributed in the cytoplasm throughout fungal development.
- Taxon
- wheatTriticum aestivume2
- Organ / stage
- different stages of fungal developmente1
- Gene
- FgCpc2e1
- Outcome
- uniformly distributed in the cytoplasme1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“FgCpc2 is uniformly distributed in the cytoplasm throughout different stages of fungal development”
e2“reduced disease symptoms on wheat coleoptiles and flowering heads”
Claim · model-written summary
FgCpc2 is highly conserved across species and shares notable amino acid similarity with Gβ-like proteins.
- Taxon
- wheatTriticum aestivume3
- Gene
- FgCpc2e1
- Outcome
- highly conservede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Phylogenetic analysis reveals that FgCpc2 is highly conserved across various species”
e2“shares notable amino acid sequence similarity with Gβ-like proteins”
e3“reduced disease symptoms on wheat coleoptiles and flowering heads”