Abstract
Abstract Roses are globally important ornamental crops worldwide and are widely used as landscape plants in outdoor greening. However, cold stress limits their growth, development, and cultivation range, especially in northern and high-altitude regions. Nevertheless, the transcriptional networks mediating cold stress responses in roses have yet to be fully understood. In this study, we identified and characterized RcWRKY71, a cold-induced WRKY transcription factor from rose (Rosa chinensis). Our findings systematically establish that RcWRKY71 is a key positive regulator of cold hardiness in roses. Overexpressing of RcWRKY71 enhanced cold resistance, as indicated by improved growth performance of callus under low-temperature conditions. Conversely, silencing this gene reduced cold tolerance, resulting in reduced leaf photosynthetic capacity and lower soluble sugar content in roots. Furthermore, we provide conclusive evidence that RcWRKY71 directly binds to and transcriptionally activates its downstream gene RcGolS1. Virus-induced gene silencing (VIGS) of RcGolS1 led to reduced cold tolerance, while its overexpression increased cold resistance by activating raffinose family oligosaccharide (RFOs) biosynthesis, as evidenced by the accumulation of myo-inositol, galactinol, and raffinose. Our study reveals a novel RcWRKY71-RcGolS1 regulatory module within the cold hardiness signaling pathway and provides key molecular evidence explaining how cryoprotective oligosaccharides accumulate to facilitate overwintering adaptation in perennial woody plants. These findings provide valuable genetic resources and a theoretical foundation for breeding new, cold-resistant rose germplasm through genetic engineering.
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Claims 8
Claim · model-written summary
RcWRKY71 was identified as a cold-induced transcription factor in rose.
Verbatim
e0“RcWRKY71, a cold-induced WRKY transcription factor from rose (Rosa chinensis)”
Claim · model-written summary
RcWRKY71 overexpression improved callus growth and cold resistance at low temperature.
- Taxon
- rosee1
- Organ / stage
- calluse0
- Treatment
- Overexpressing of RcWRKY71e0
- Gene
- RcWRKY71e0
- Outcome
- growth performance of calluse0
- Direction
- improvede0
- Qualifier
- low-temperature conditionse0
Not reportedComparator
Verbatim
e0“Overexpressing of RcWRKY71 enhanced cold resistance, as indicated by improved growth performance of callus under low-temperature conditions”
e1“rose (Rosa chinensis)”
Claim · model-written summary
RcWRKY71 silencing reduced leaf photosynthetic capacity under cold stress.
- Taxon
- rosee2
- Organ / stage
- leafe0
- Treatment
- silencing this genee0
- Gene
- RcWRKY71e1
- Outcome
- leaf photosynthetic capacitye0
- Direction
- reducede0
Not reportedComparator
Verbatim
e0“silencing this gene reduced cold tolerance, resulting in reduced leaf photosynthetic capacity and lower soluble sugar content in roots”
e1“characterized RcWRKY71”
e2“rose (Rosa chinensis)”
Claim · model-written summary
RcWRKY71 silencing lowered root soluble sugar content.
- Taxon
- rosee2
- Organ / stage
- rootse0
- Treatment
- silencing this genee0
- Gene
- RcWRKY71e1
- Outcome
- soluble sugar contente0
- Direction
- lowere0
Not reportedComparator
Verbatim
e0“silencing this gene reduced cold tolerance, resulting in reduced leaf photosynthetic capacity and lower soluble sugar content in roots”
e1“characterized RcWRKY71”
e2“rose (Rosa chinensis)”
Claim · model-written summary
RcWRKY71 directly binds and activates RcGolS1.
Verbatim
e0“RcWRKY71 directly binds to and transcriptionally activates its downstream gene RcGolS1”
e1“rose (Rosa chinensis)”
Claim · model-written summary
RcGolS1 silencing reduced rose cold tolerance.
- Taxon
- rosee1
- Treatment
- Virus-induced gene silencing (VIGS) of RcGolS1e0
- Gene
- RcGolS1e0
- Outcome
- cold tolerancee0
- Direction
- reducede0
Not reportedComparator
Verbatim
e0“Virus-induced gene silencing (VIGS) of RcGolS1 led to reduced cold tolerance”
e1“rose (Rosa chinensis)”
Claim · model-written summary
RcGolS1 overexpression increased cold resistance through raffinose-family oligosaccharide biosynthesis.
- Taxon
- rosee2
- Treatment
- its overexpressione0
- Gene
- RcGolS1e1
- Outcome
- cold resistancee0
- Direction
- increasede0
- Qualifier
- activating raffinose family oligosaccharide (RFOs) biosynthesise0
Not reportedComparator
Verbatim
e0“its overexpression increased cold resistance by activating raffinose family oligosaccharide (RFOs) biosynthesis”
e1“silencing (VIGS) of RcGolS1”
e2“rose (Rosa chinensis)”
Claim · model-written summary
RcGolS1 overexpression accumulated myo-inositol, galactinol, and raffinose.
- Taxon
- rosee3
- Treatment
- its overexpressione1
- Gene
- RcGolS1e2
- Outcome
- accumulation of myo-inositol, galactinol, and raffinosee0
- Direction
- accumulatione0
Not reportedComparator
Verbatim
e0“as evidenced by the accumulation of myo-inositol, galactinol, and raffinose”
e1“its overexpression increased cold resistance by activating raffinose family oligosaccharide (RFOs) biosynthesis”
e2“silencing (VIGS) of RcGolS1”
e3“rose (Rosa chinensis)”
The same questions in other papers
loss of function · tolerance: abiotic · RosaResults agree
3 papers in the same direction as this paper
- negative
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.