Abstract
NAC (NAM, ATAF1,2, and CUC2) transcription factors (TFs) play critical roles in controlling plant growth, development, and abiotic stress responses. However, few studies have examined NAC proteins related to drought stress tolerance in rose (Rosa chinensis). Here, we identified a drought- and abscisic acid (ABA)-induced NAC TF, RcNAC091, that localizes to the nucleus and has transcriptional activation activity. Virus-induced silencing of RcNAC091 resulted in decreased drought stress tolerance, and RcNAC091 overexpression had the opposite effect. Specifically, ABA mediated RcNAC091-regulated drought tolerance. A transcriptomic comparison showed altered expression of genes involved in ABA signaling and oxidase metabolism in RcNAC091-silenced plants. We further confirmed that RcNAC091 directly targets the promoter of RcWRKY71 in vivo and in vitro. Moreover, RcWRKY71-slienced rose plants were not sensitive to both ABA and drought stress, whereas RcWRKY71-overexpressing plants were hypersensitive to ABA, which resulted in drought-tolerant phenotypes. The expression of ABA biosynthesis- and signaling-related genes was impaired in RcWRKY71-slienced plants, suggesting that RcWRKY71 might facilitate the ABA-dependent pathway. Therefore, our results show that RcWRKY71 is transcriptionally activated by RcNAC091, which positively modulates ABA signaling and drought responses. The results of this study provide insights into the roles of TFs as functional links between RcNAC091 and RcWRKY71 in priming resistance; our findings also have implications for the approaches to enhance the drought resistance of roses.
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Claims 9
Claim · model-written summary
RcNAC091 is induced by drought and ABA and has nuclear localization and transcriptional activation activity.
Verbatim
e0“we identified a drought- and abscisic acid (ABA)-induced NAC TF, RcNAC091, that localizes to the nucleus and has transcriptional activation activity.”
t0“rose”
Claim · model-written summary
Silencing RcNAC091 decreased rose drought tolerance.
- Taxon
- roset0
- Treatment
- Virus-induced silencing of RcNAC091e0
- Gene
- RcNAC091e0
- Outcome
- drought stress tolerancee0
- Direction
- decreasede0
Not reportedComparator
Verbatim
e0“Virus-induced silencing of RcNAC091 resulted in decreased drought stress tolerance”
t0“rose”
Claim · model-written summary
Overexpressing RcNAC091 increased rose drought tolerance.
Not reportedComparatorDirection
Verbatim
e0“RcNAC091 overexpression had the opposite effect.”
t0“rose”
Claim · model-written summary
ABA mediated RcNAC091-regulated drought tolerance.
Verbatim
e0“Specifically, ABA mediated RcNAC091-regulated drought tolerance.”
t0“rose”
Claim · model-written summary
Silencing RcNAC091 altered expression of genes in ABA signaling and oxidase metabolism.
Verbatim
e0“A transcriptomic comparison showed altered expression of genes involved in ABA signaling and oxidase metabolism in RcNAC091-silenced plants.”
t0“rose”
Claim · model-written summary
RcNAC091 directly targets the RcWRKY71 promoter.
Verbatim
e0“RcNAC091 directly targets the promoter of RcWRKY71 in vivo and in vitro.”
t0“rose”
Claim · model-written summary
RcWRKY71-silenced rose plants were insensitive to ABA and drought stress.
Verbatim
e0“RcWRKY71-slienced rose plants were not sensitive to both ABA and drought stress”
Claim · model-written summary
RcWRKY71 overexpression made rose plants hypersensitive to ABA and drought tolerant.
Verbatim
e0“RcWRKY71-overexpressing plants were hypersensitive to ABA, which resulted in drought-tolerant phenotypes.”
t0“rose”
Claim · model-written summary
ABA biosynthesis and signaling gene expression was impaired in RcWRKY71-silenced plants.
Verbatim
e0“The expression of ABA biosynthesis- and signaling-related genes was impaired in RcWRKY71-slienced plants”
t0“rose”
The same questions in other papers
loss of function · tolerance: abiotic · RosaResults agree
3 papers in the same direction as this paper
- negative
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.