Abstract
Mediator is a multiprotein complex integral to the transcription machinery, mediated by RNA polymerase II. Some Mediator subunits have been found to have critical functions in plants’ responses to abiotic stresses. However, the role of plant Mediator subunits in drought responses remains largely enigmatic. Here, we identified a Mediator subunit, RhMED15a, in roses (Rosa hybrida). Its expression was greatly and swiftly induced by dehydration treatment in the root. The promoter sequence of RhMED15a contains cis-acting elements that respond to abscisic acid (ABA) and methyl jasmonate (MeJA). In addition, the expression of RhMED15a was significantly up-regulated with ABA treatment and inversely down-regulated with MeJA treatment. Silencing RhMED15a using virus-induced gene silencing (VIGS) in roses significantly reduced drought tolerance in rose plants. This resulted in a significant increase in the malondialdehyde (MDA) level and a decreased survival rate in comparison to TRV controls. Moreover, we found that the expression of five drought-related genes, including dehydration responsive element binding factor 1B (DREB1B), responsive to desiccation stress 29A (RD29A), responsive to desiccation stress 29B (RD29B), early response to dehydration 14 (ERD14), and 9-cis-epoxycarotenoid dioxygenase 1 (NCED1), was considerably suppressed in RhMED15a-silenced plants during drought stress. Taken together, our results present that the Mediator tail module subunit RhMED15a serves as an enhancer of drought tolerance in rose, probably through the modulation of the expression of some drought-related genes.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
RhMED15a was identified in roses.
Verbatim
e0“we identified a Mediator subunit, RhMED15a, in roses (Rosa hybrida)”
Claim · model-written summary
Dehydration rapidly induced root RhMED15a expression.
- Taxon
- Roset0
- Organ / stage
- roote0
- Treatment
- dehydration treatmente0
- Gene
- RhMED15at0
- Outcome
- expressione0
- Direction
- inducede0
Not reportedComparator
Verbatim
e0“Its expression was greatly and swiftly induced by dehydration treatment in the root”
t0“RhMED15a Regulates Drought Tolerance in Rose”
Claim · model-written summary
The RhMED15a promoter contains ABA and MeJA responsive elements.
Verbatim
e0“The promoter sequence of RhMED15a contains cis-acting elements that respond to abscisic acid (ABA) and methyl jasmonate (MeJA)”
t0“Rose”
Claim · model-written summary
ABA treatment increased RhMED15a expression.
Not reportedComparator
Verbatim
e0“the expression of RhMED15a was significantly up-regulated with ABA treatment”
t0“Rose”
Claim · model-written summary
MeJA treatment decreased RhMED15a expression.
Not reportedComparator
Verbatim
e0“the expression of RhMED15a was significantly up-regulated with ABA treatment and inversely down-regulated with MeJA treatment”
t0“Rose”
Claim · model-written summary
RhMED15a silencing reduced drought tolerance.
- Taxon
- rosese0
- Treatment
- Silencing RhMED15a using virus-induced gene silencing (VIGS)e0
- Gene
- RhMED15ae0
- Outcome
- drought tolerancee0
- Direction
- reducede0
Not reportedComparator
Verbatim
e0“Silencing RhMED15a using virus-induced gene silencing (VIGS) in roses significantly reduced drought tolerance in rose plants”
Claim · model-written summary
RhMED15a silencing increased malondialdehyde during drought relative to TRV controls.
Verbatim
e0“This resulted in a significant increase in the malondialdehyde (MDA) level and a decreased survival rate in comparison to TRV controls”
t0“RhMED15a Regulates Drought Tolerance in Rose”
Claim · model-written summary
RhMED15a silencing decreased survival relative to TRV controls.
Verbatim
e0“a decreased survival rate in comparison to TRV controls”
t0“RhMED15a Regulates Drought Tolerance in Rose”
Claim · model-written summary
RhMED15a silencing suppressed expression of five drought-related genes during drought.
- Taxon
- Roset0
- Treatment
- RhMED15a-silencede0
- Outcome
- expression of five drought-related genese0
- Direction
- suppressede0
- Qualifier
- during drought stresse0
Not reportedComparator
Verbatim
e0“the expression of five drought-related genes, including dehydration responsive element binding factor 1B (DREB1B), responsive to desiccation stress 29A (RD29A), responsive to desiccation stress 29B (RD29B), early response to dehydration 14 (ERD14), and 9-cis-epoxycarotenoid dioxygenase 1 (NCED1), was considerably suppressed in RhMED15a-silenced plants during drought stress”
t0“Rose”
Claim · model-written summary
The authors interpret RhMED15a as enhancing rose drought tolerance, probably via drought-gene expression.
Verbatim
e0“RhMED15a serves as an enhancer of drought tolerance in rose, probably through the modulation of the expression of some drought-related genes”
The same questions in other papers
drought · molecular: expression · RosaPositive and negative results
3 in the opposite direction
- negative
- negative
- no effect
loss of function · tolerance: abiotic · RosaResults agree
3 papers in the same direction as this paper
- negative
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.