Abstract
The global warming and its effect on sea level raise is an undisputed fact that has been supported by numerous studies. Countries such as Belize with large area of land just above sea level will face problems due to increased soil salinity. The sea level raise will also affect our fresh water supplies due to back flow in to our rivers. These all will have effect on agriculture since many crops cannot tolerate the increased salinity level. Development of resistant varieties of crops that can withstand moderate level of salinity can help to mitigate the problems of food shortage in the future. Tissue culture or micropropagation can be an effective technique for the in vitro development of salt resistant varieties of crops. Initially an experiment was carried out to test the effect of hormones on callus formation of red kidney beans (Phaseolus vulgaris L.). Callus tissue was initiated on MS medium supplemented with various hormones (IAA, IBA and 2,4 D) by using explants from epicotyle. Based on the fresh weight produced during the incubation period, 2,4-D was identified as the best hormone for callus growth. After choosing the best hormone for callus generation under in vitro conditions, the effect of salinity on callus regeneration and organogenesis of P. vulgaris was tested under in vitro conditions. Although callus regeneration occurred in all of the salt concentration used in the experiment, the callus regeneration decreased as the concentration of the salt increased. Microscopic evidence of organogenesis was observed as the callus tissue has differentiated in to roots, root hairs and vascular tissues under in vitro saline conditions.
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Claims 4
Claim · model-written summary
2,4-D was the best hormone for red kidney bean callus growth, based on fresh weight, compared with IAA and IBA.
Verbatim
e1“Based on the fresh weight produced during the incubation period, 2,4-D was identified as the best hormone for callus growth”
e2“Callus tissue was initiated on MS medium supplemented with various hormones (IAA, IBA and 2,4 D) by using explants from epicotyle”
e3“The effect of hormones on callus formation of red kidney beans (Phaseolus vulgaris L.)”
Claim · model-written summary
Salinity decreased callus regeneration of P. vulgaris as the salt concentration increased.
- Taxon
- P. vulgaris
Phaseolus vulgarise5 - Treatment
- salte4
- Outcome
- callus regeneratione4
- Direction
- decreasede4
- Qualifier
- as the concentration of the salt increasede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“the callus regeneration decreased as the concentration of the salt increased”
e5“the effect of salinity on callus regeneration and organogenesis of P. vulgaris was tested”
Claim · model-written summary
Callus of P. vulgaris regenerated at all salt concentration used in the experiment.
- Taxon
- P. vulgarisPhaseolus vulgarise5
- Outcome
- callus regeneratione6
- Qualifier
- in all of the salt concentration used in the experimente6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“the effect of salinity on callus regeneration and organogenesis of P. vulgaris was tested”
e6“Although callus regeneration occurred in all of the salt concentration used in the experiment”
Claim · model-written summary
Callus of P. vulgaris differentiated into roots, root hairs and vascular tissues under in vitro saline conditions, with microscopic evidence of organogenesis.
- Taxon
- P. vulgarisPhaseolus vulgarise5
- Organ / stage
- roots, root hairs and vascular tissuese7
- Treatment
- saline conditionse7
- Outcome
- organogenesise7
- Setting
- in vitro saline conditionse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“the effect of salinity on callus regeneration and organogenesis of P. vulgaris was tested”
e7“Microscopic evidence of organogenesis was observed as the callus tissue has differentiated in to roots, root hairs and vascular tissues under in vitro saline conditions”
The same questions in other papers
2,4-D · growth · Phaseolus vulgaris (common bean)Results differ
8 papers in the same direction as this paper · 9 in the opposite direction
- negative
- negative
- negative
14 papers more in this group
See all 18 papers →salinity · growth · Phaseolus vulgaris (common bean)Results differ
63 papers in the same direction as this paper · 7 in the opposite direction
- positive
- positive
- positive
67 papers more in this group
See all 71 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.