Paper · 1952 · Annals of Applied Biology

SOME EFFECTS OF NITROGEN NUTRITION ON THE MORPHOLOGY AND ANATOMY OF MARROW‐STEM KALE

S. E. ARNEY

14
claims
23
verbatim quotes
2
citations
3
research areas
—
Not open access

Abstract

Marrow‐stem kale plants grown on plots receiving frequent additions of sulphate of ammonia showed a 40% increase in length of internode and a 25% increase in number of nodes per plant, and the leaf size was increased by between 50 and 70% over plants in plots receiving no N fertilizer. Leaves of kale continue to increase in area until they turn yellow, and the high N leaves showed a greater rate of increase in area at every stage in the life of the leaf. Various features of leaf structure, such as stomatal index, and thickness of palisade and mesophyll, were unaffected by N treatment. The size of the epidermal cells of the leaves was very variable, and although the high N leaves showed a 12% increase in area per epidermal cell over the low N leaves, this difference is not statistically significant. The increased area of the high N leaves can therefore be attributed mainly to increased cell division during the life of the leaf. Only a very slight increase in rate of cell division is necessary to produce the observed effect. The greater leaf area of the high N plants can be attributed mainly to increased size of individual leaves, but there was also a significantly greater number of living functional leaves per plant on the high N plants; at 23 weeks from sowing the high N plants had an average of 13.4 living leaves, while the low N plants had only 11.7 living leaves per plant. There was an appreciable degree of N succulence in the high N kale leaves, which showed a 2% greater moisture content than the low N leaves. A seasonal drift in epidermal cell size, palisade thickness, and total leaf thickness, is shown to be fully significant, statistically. Marked variations in stomatal frequency are barely significant at the 5% level.

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Claims 14

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Sulphate of ammonia increased internode length by 40%.

Taxon
Marrow‐stem kaleBrassica oleracea var. medullosae0
Treatment
frequent additions of sulphate of ammoniae0
Outcome
length of internodee0
Direction
increasee0
Value
40%e0
Comparator
no N fertilizere0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. medullosa
Brassica oleracea var. medullosa · TaxonMPF · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Marrow‐stem kale plants grown on plots receiving frequent additions of sulphate of ammonia showed a 40% increase in length of internode and a 25% increase in number of nodes per plant, and the leaf size was increased by between 50 and 70% over plants in plots receiving no N fertilizer.”

✓ located verbatim in the source · abstract, characters 0–286 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Sulphate of ammonia increased nodes per plant by 25%.

Taxon
Marrow‐stem kaleBrassica oleracea var. medullosae0
Treatment
frequent additions of sulphate of ammoniae0
Outcome
number of nodes per plante0
Direction
increasee0
Value
25%e0
Comparator
no N fertilizere0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. medullosa
Brassica oleracea var. medullosa · TaxonMPF · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Marrow‐stem kale plants grown on plots receiving frequent additions of sulphate of ammonia showed a 40% increase in length of internode and a 25% increase in number of nodes per plant, and the leaf size was increased by between 50 and 70% over plants in plots receiving no N fertilizer.”

✓ located verbatim in the source · abstract, characters 0–286 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Sulphate of ammonia increased leaf size by 50 to 70%.

Taxon
Marrow‐stem kaleBrassica oleracea var. medullosae0
Treatment
frequent additions of sulphate of ammoniae0
Outcome
leaf sizee0
Direction
increasede0
Value
between 50 and 70%e0
Comparator
no N fertilizere0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. medullosa
Brassica oleracea var. medullosa · TaxonMPF · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Marrow‐stem kale plants grown on plots receiving frequent additions of sulphate of ammonia showed a 40% increase in length of internode and a 25% increase in number of nodes per plant, and the leaf size was increased by between 50 and 70% over plants in plots receiving no N fertilizer.”

✓ located verbatim in the source · abstract, characters 0–286 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Kale leaves kept increasing in area until yellowing.

Outcome
increase in areae0
Direction
increasee0
Qualifier
until they turn yellowe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. viridis
Brassica oleracea var. viridis · TaxonAnn Perry · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Leaves of kale continue to increase in area until they turn yellow”

✓ located verbatim in the source · abstract, characters 287–353 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

High N leaves increased in area faster at every leaf stage.

Treatment
high Ne0
Outcome
rate of increase in areae0
Direction
greatere0
Qualifier
at every stage in the life of the leafe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. viridis
Brassica oleracea var. viridis · TaxonAnn Perry · Public domain · Wikimedia Commons ↗

Verbatim

e0

“the high N leaves showed a greater rate of increase in area at every stage in the life of the leaf.”

✓ located verbatim in the source · abstract, characters 359–458 · show in text ↑
t0

“MARROW‐STEM KALE”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Nitrogen treatment did not affect stomatal index or palisade and mesophyll thickness.

Treatment
N treatmente0
Outcome
stomatal index, and thickness of palisade and mesophylle0
Direction
unaffectede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. viridis
Brassica oleracea var. viridis · TaxonAnn Perry · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Various features of leaf structure, such as stomatal index, and thickness of palisade and mesophyll, were unaffected by N treatment.”

✓ located verbatim in the source · abstract, characters 459–591 · show in text ↑
t0

“MARROW‐STEM KALE”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
Intervention effectobservedoriginal resultpositivenot significant (as stated)measured

Claim · model-written summary

High N epidermal cell area was 12% higher, but the difference was not significant.

Treatment
high Ne0
Outcome
area per epidermal celle0
Direction
increasee0
Value
12%e0
Comparator
low Ne0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. viridis
Brassica oleracea var. viridis · TaxonAnn Perry · Public domain · Wikimedia Commons ↗

Verbatim

e0

“although the high N leaves showed a 12% increase in area per epidermal cell over the low N leaves, this difference is not statistically significant.”

✓ located verbatim in the source · abstract, characters 661–809 · show in text ↑
t0

“MARROW‐STEM KALE”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Authors attribute greater leaf area chiefly to cell division.

Outcome
increased areae0
Qualifier
increased cell division during the life of the leafe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. viridis
Brassica oleracea var. viridis · TaxonAnn Perry · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The increased area of the high N leaves can therefore be attributed mainly to increased cell division during the life of the leaf.”

✓ located verbatim in the source · abstract, characters 810–940 · show in text ↑
t0

“MARROW‐STEM KALE”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Authors say a slight increase in cell division rate suffices for the observed effect.

Outcome
observed effecte0
Qualifier
very slight increase in rate of cell divisione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. viridis
Brassica oleracea var. viridis · TaxonAnn Perry · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Only a very slight increase in rate of cell division is necessary to produce the observed effect.”

✓ located verbatim in the source · abstract, characters 941–1038 · show in text ↑
t0

“MARROW‐STEM KALE”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Authors attribute greater leaf area mainly to larger individual leaves.

Outcome
greater leaf areae0
Qualifier
increased size of individual leavese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. viridis
Brassica oleracea var. viridis · TaxonAnn Perry · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The greater leaf area of the high N plants can be attributed mainly to increased size of individual leaves”

✓ located verbatim in the source · abstract, characters 1039–1145 · show in text ↑
t0

“MARROW‐STEM KALE”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

High N plants had significantly more living functional leaves, averaging 13.4 versus 11.7 at 23 weeks.

Treatment
high Ne0
Outcome
number of living functional leaves per plante0
Direction
greatere0
Value
13.4 living leavese0
Qualifier
11.7 living leaves per plante0
Comparator
low Ne0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. viridis
Brassica oleracea var. viridis · TaxonAnn Perry · Public domain · Wikimedia Commons ↗

Verbatim

e0

“there was also a significantly greater number of living functional leaves per plant on the high N plants; at 23 weeks from sowing the high N plants had an average of 13.4 living leaves, while the low N plants had only 11.7 living leaves per plant.”

✓ located verbatim in the source · abstract, characters 1151–1398 · show in text ↑
t0

“MARROW‐STEM KALE”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

High N leaves had 2% greater moisture than low N leaves.

Treatment
high Ne0
Outcome
moisture contente0
Direction
greatere0
Value
2%e0
Comparator
low Ne0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. viridis
Brassica oleracea var. viridis · TaxonAnn Perry · Public domain · Wikimedia Commons ↗

Verbatim

e0

“high N kale leaves, which showed a 2% greater moisture content than the low N leaves.”

✓ located verbatim in the source · abstract, characters 1454–1539 · show in text ↑
Associationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Epidermal cell size, palisade thickness and total leaf thickness varied significantly seasonally.

Outcome
epidermal cell size, palisade thickness, and total leaf thicknesse0
Direction
drifte0
Setting
seasonale0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. viridis
Brassica oleracea var. viridis · TaxonAnn Perry · Public domain · Wikimedia Commons ↗

Verbatim

e0

“A seasonal drift in epidermal cell size, palisade thickness, and total leaf thickness, is shown to be fully significant, statistically.”

✓ located verbatim in the source · abstract, characters 1540–1675 · show in text ↑
t0

“MARROW‐STEM KALE”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
Observationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Stomatal frequency variation was barely significant at 5%.

Outcome
variations in stomatal frequencye0
Value
5%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. viridis
Brassica oleracea var. viridis · TaxonAnn Perry · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Marked variations in stomatal frequency are barely significant at the 5% level.”

✓ located verbatim in the source · abstract, characters 1676–1755 · show in text ↑
t0

“MARROW‐STEM KALE”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑

ammonium sulfate · yield · Brassica oleracea (wild cabbage)Positive and negative results

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5 papers in the same direction as this paper · 1 reporting no difference from an alternative

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ammonium sulfate · growth · Brassica oleracea (wild cabbage)Results agree

2 papers: 2 positive, 0 negative or no effect

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nitrogen · quality: grain · Brassica oleracea (wild cabbage)Positive and negative results

7 papers: 5 positive, 2 negative or no effect

4 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. negative
  3. positive

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nitrogen · growth · Brassica oleracea (wild cabbage)Positive and negative results

103 papers: 90 positive, 13 negative or no effect

89 papers in the same direction as this paper · 12 in the opposite direction · 1 reporting no difference from an alternative

  1. no effect
  2. no effect
  3. no effect

99 papers more in this group

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.