Abstract
To determine which components of the plant defense response make important contributions to limiting pathogen attack, an M(2) mutagenized population of a transgenic Arabidopsis line was screened for mutants showing constitutive expression of beta-glucuronidase activity driven by the promoter region of the CEVI-1 gene. The CEVI-1 gene originally was isolated from tomato plants and has been shown to be induced in susceptible varieties of tomato plants by virus infection in a salicylic acid-independent manner. We report here the characterization of a recessive mutant, detachment9 (dth9). This mutant is more susceptible to both virulent and avirulent forms of the oomycete Peronospora and also exhibits increased susceptibility to the moderately virulent bacterial pathogen Pseudomonas syringae pv maculicola ES4326. However, this mutant is not affected in salicylic acid metabolism and shows normal expression of pathogenesis-related (PR) genes after pathogen attack. Furthermore, after inoculation with avirulent pathogens, the dth9 mutant shows a compromised systemic acquired resistance response that cannot be complemented by exogenous application of salicylic acid, although this molecule is able to promote normal activation of PR genes. Therefore, the dth9 mutation defines a regulator of disease susceptibility that operates upstream or independently of salicylic acid. Pleiotropy is also evident in the dth9 mutant in the sense that the shoots of dth9 plants are insensitive to the exogenously applied auxin analog 2,4-dichlorophenoxyacetic acid.
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Claims 9
Claim · model-written summary
dth9 was more susceptible to virulent Peronospora.
- Taxon
- Arabidopsis
Arabidopsist0 - Material
- dth9t0
- Interactor
- Peronospora
Peronosporae0pathogen - Outcome
- susceptiblee0
- Direction
- moree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This mutant is more susceptible to both virulent and avirulent forms of the oomycete Peronospora”
t0“Arabidopsis dth9”
Claim · model-written summary
dth9 was more susceptible to avirulent Peronospora.
- Taxon
- ArabidopsisArabidopsist0
- Material
- dth9t0
- Interactor
- PeronosporaPeronosporae0pathogen
- Outcome
- susceptiblee0
- Direction
- moree0
- Qualifier
- avirulente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This mutant is more susceptible to both virulent and avirulent forms of the oomycete Peronospora”
t0“Arabidopsis dth9”
Claim · model-written summary
dth9 had increased susceptibility to Pseudomonas syringae pv maculicola ES4326.
- Taxon
- ArabidopsisArabidopsist0
- Material
- dth9t0
- Interactor
- Pseudomonas syringae pv maculicola ES4326
Pseudomonas syringaee0pathogen - Outcome
- susceptibilitye0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“also exhibits increased susceptibility to the moderately virulent bacterial pathogen Pseudomonas syringae pv maculicola ES4326.”
t0“Arabidopsis dth9”
Claim · model-written summary
dth9 salicylic acid metabolism was unaffected.
- Taxon
- ArabidopsisArabidopsist0
- Material
- dth9t0
- Outcome
- salicylic acid metabolisme0
- Direction
- not affectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this mutant is not affected in salicylic acid metabolism”
t0“Arabidopsis dth9”
Claim · model-written summary
dth9 retained normal PR gene expression after pathogen attack.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- pathogenesis-related (PR) genese0
- Outcome
- expression of pathogenesis-related (PR) genese0
- Direction
- normale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shows normal expression of pathogenesis-related (PR) genes after pathogen attack.”
t0“Arabidopsis dth9”
Claim · model-written summary
dth9 had compromised systemic acquired resistance after avirulent pathogen inoculation.
- Taxon
- ArabidopsisArabidopsist0
- Material
- dth9e0
- Treatment
- inoculation with avirulent pathogense0
- Outcome
- systemic acquired resistance responsee0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after inoculation with avirulent pathogens, the dth9 mutant shows a compromised systemic acquired resistance response”
t0“Arabidopsis dth9”
Claim · model-written summary
Exogenous salicylic acid did not complement compromised systemic acquired resistance in dth9.
- Taxon
- ArabidopsisArabidopsist0
- Material
- dth9e0
- Treatment
- exogenous application of salicylic acide0
- Outcome
- systemic acquired resistance responsee0
- Direction
- cannot be complementede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the dth9 mutant shows a compromised systemic acquired resistance response that cannot be complemented by exogenous application of salicylic acid”
t0“Arabidopsis dth9”
Claim · model-written summary
Exogenous salicylic acid promoted normal PR gene activation in dth9.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- salicylic acide0
- Gene
- PR genese0
- Outcome
- activation of PR genese0
- Direction
- promotee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous application of salicylic acid, although this molecule is able to promote normal activation of PR genes.”
t0“Arabidopsis dth9”
Claim · model-written summary
dth9 shoots were insensitive to exogenously applied 2,4-dichlorophenoxyacetic acid.
- Taxon
- ArabidopsisArabidopsist0
- Material
- dth9e0
- Organ / stage
- shootse0
- Treatment
- exogenously applied auxin analog 2,4-dichlorophenoxyacetic acide0
- Outcome
- insensitivee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the shoots of dth9 plants are insensitive to the exogenously applied auxin analog 2,4-dichlorophenoxyacetic acid.”
t0“Arabidopsis dth9”
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