Abstract
Received: 2015-10-03 | Accepted: 2016-03-03 | Available online: 2016-05-30 dx.doi.org/10.15414/afz.2016.19.02.45-50 Effect of split application of nitrogen and nitrogen+sulphur nutrition in comparison with one shot application of nitrogen + sulphur + inhibitors of nitrification (dikyandiamid, DCD and 1, 2, 4 triazol,TZ) on the yield and quality of maize grain was examined in three-year small-plot experiment with maize (hybrid ,Chapalu FAO 350) on medium heavy degraded brownsoil. On the average of three experimental years one-shot application of nitrogen-sulphur nutrition in the form of ENSIN fertilizer containing nitrification inhibitors applied before maize seeding at the rate of 160 kg.ha -1 N and 80 kg.ha -1 S showed the most considerable effect on all investigated parameters. Yield of grain was increased highly significantly by 1.56 t.ha -1 (+21.8%) against control treatment. Both parameters TKW (thousand kernel weight) and content of crude protein were elevated by 7.9% and 8.3%, respectively. Production of protein per hectare was increased by 253 kg, that is by 31.9% and natural effectiveness of fertilization achieved the highest value, namely 9.8 kg of grain per 1 kg of applied nitrogen. When fertilizer ENSIN (contains inhibitors of nitrification) was applied in maize nutrition the achieved results were insignificantly better in all examined parameters in comparison with the split application of DASA 26/13 fertilizer under the same rate of both nitrogen and sulphur. There was found out positive tendency of favourable effect on yield and quality of grain of maize and decrease of application costs as a consequence of one shot application in this treatment. Addition of sulphur to nitrogen as well as an addition of inhibitors + sulphur to nitrogen significantly increased only grain yield of maize. Content of crude protein and TKW were increased insignificantly. Natural effectiveness of nitrogen fertilization was increasing in accordance with raising yields of maize grain as follows: nitrogen < nitrogen + sulphur < nitrogen + sulphur + inhibitors. Keywords: growth stage, inhibitors, nitrification, nitrogen, split References Asing, J. et al. (2008) Assessment of nitrogen losses from urea and an organic manure with and without nitrification inhibitor, dicyandiamide, applied to lettuce under glasshouse conditions. Australian Journal of Soil Research, vol. 46, pp. 535–541. Beever, D. et al. (2007) Sustainable Management of the Nitrogen Cycle in Agriculture and Mitigation of Reactive Nitrogen Side Effects. International Fertilizer Industry Association , ISBN 2-9523139-1-1, www.fertilizer.org/ifa/publicat/pdf/2007_ifa_reactive%20nitrogen.pdf Bergmann, W. and Neubert, P. (1976) Pflanzendiagnose und Pflanzenanalyze. Jena: VEBC Fischer, 711 p. Cassmann, K.G., Dobermann, A. and Walters, D.T. (2002) Agroecosystems, nitrogen use efficiency, and nitrogen management. Ambio , vol. 31, no. 2 , pp. 132-140. COHEN, J. B. (1910) Practical Organic Chemistry. London: MacMillan, 356 p. Di, H.J. and Cameron, K.C. (2002) Nitrate leaching in temperate agroecosystems: sources, factors and mitigating strategies. Nutrient Cycling in Agroecosystems , vol. 46 , pp. 237-256. Di, H.J. and Cameron, K.C. (2004) Effects of the nitrification inhibitor dicyandiamide on potassium, magnesium and calcium leaching in grazed grassland . Soil use and management . vol. 20, pp. 2-7 Di, H.J. et al. (2010) Ammonia oxidizing bacteria and archaea grow under contrasting soil nitrogen conditions. FEMS Microbiology Ecology , vol. 72 , pp. 386–394. Dobermann, A. (2005) Nitrogen Use Efficiency – State of the Art. IFA International Workshop on Enhanced–Efficiency Fertilizers, www.fertilizer.org/ifa/publicat/PDF/2005_ag_frankfurt_doberman.pdf Fecenko, J. and Ložek, O. (2000) Nutrition and fertilization of field crops . Nitra: SPU v Nitre and Duslo, a.s. Šaľa. 442 p. (in Slovak) Galloway, J.N. et al. ( 2003) The Nitrogen Cascade. BioScience, vol. 53, no. 4, pp. 341-356. Hanáčková, E. and Žembery, J. (2015) Effect of selected factors on the grain maize yield. Agrochémia (Agrochemistry ), vol. 19(55), no. 1, pp. 6-13. (in Slovak) Ivanič, J., Havelka, B. and Knop, K. (1984) Nutrition and fertilization of plants . Príroda Bratislava, SZN Praha, 255 p.(in Slovak). Khalil, M. I., Gutser, R. and Schmidhalter, U.(2009) Effects of urease and nitrification inhibitors added to urea on nitrous oxide emissions from a loess soil . J. Plant Nutr. Soil Sci. vol. 172 , pp. 651–660. Kramer, S.B. et al. (2007) Reduced nitrate leaching and enhanced denitrifier activity and efficiency in organically fertilizer soils. Proceedings of National Academy of Science , vol. 103 , pp. 4522-4527. Laboski, C. (2006) Does it pay to use nitrification and urease inhibitors?: Proc. of the 2006 Wisconsin Fertilizer, Aglime & Pest Management Conference , vol. 45, pp . 44-50 Ladha, J.K., et al. (2005) Efficiency of fertilizer nitrogen in cereal production: Retrospects and prospects. Advances in Agronomy , vol. 87, pp . 85-156. Ledgard, S. F. (2001) Nitrogen cycling in low input legume-based agriculture, withemphasis on legume/grass pastures. Plant and Soil , vol. 228 , pp . 43-59 Macadam, X.M.B. et al. (2003) Dicyandiamide and 3,4-dimethyl pyrazole phosphate decrease N 2 O emissions from grassland but dicyandiamide produces deleterious effects in clover. Journal of plant physiology , vol. 160 , pp . 1517-1523. Malý, S., Šarapatka, B. and Kršková, M. (2002) Seasonal variability in soil nitrogen mineralization and nitrification as influenced by N fertilization. Rostlinná výroba , vol. 48 , no . 9, pp.389-396. (in Slovak) Marendiak, P., Kopčanová, Ľ. and Leistgeb, S. (1987) Agrocultural microbiology . Príroda Bratislava, pp. 433.(in Slovak) Merino, P. et al.(2002) Mitigation of N 2 O emissions from grassland by nitrification inhibitor and Actilith F2 applied with fertilizer and cattle slurry. Soil use and management . vol. 18 , pp . 135-141. Richter, R. and Hlušek, J. (1994) Nutrition and fertilization of plants. Brno: MZLU, 177 p. (in Slovak) Richter, R. and Ryant, P. (2008) In. Zimolka, J. a kol. 2008. Maize. Proffi Press, s.r.o. Praha, pp. 111-134. (in Czech) Slamka, P., Ložek, O. and Hanáčková, E. (2014) Effect of urea containing inhibitors on nitrate portion in soil and grain yield of winter whear. Research Journal of Agricultural Science , vol. 46, no. 3, pp. 79-89 (in Slovak) Trenkel, M.E. (1997) Improving Fertilizer Use Efficiency-Controlled-Release and Stabilized Fertilizers in Agriculture . Paris: International Fertilizer Industry Association. VANĚK, V. et al. (2007) Nutrition and fertilization of field and garden crops. Praha: Profi Press, 167 p. (in Czech). VaNĚk, V. et al. (2013) Nutrition of field and garden crops. Nitra: Profi Press,175 p. (in Czech) Zimolka J. et al. (2008) Maize-main and alternative utilization. Praha: Profi Press. 200 p. (in Czech).
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Claims 10
Claim · model-written summary
One-shot ENSIN fertilization increased maize grain yield by 1.56 t.ha -1 (+21.8%) relative to control.
- Treatment
- ENSIN fertilizer containing nitrification inhibitorse0
- Outcome
- Yield of graine0
- Direction
- increasede0
- Value
- 1.56 t.ha -1 (+21.8%)e0
- Comparator
- control treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“one-shot application of nitrogen-sulphur nutrition in the form of ENSIN fertilizer containing nitrification inhibitors applied before maize seeding at the rate of 160 kg.ha -1 N and 80 kg.ha -1 S showed the most considerable effect on all investigated parameters. Yield of grain was increased highly significantly by 1.56 t.ha -1 (+21.8%) against control treatment.”
Claim · model-written summary
ENSIN fertilization elevated maize thousand kernel weight by 7.9%.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Both parameters TKW (thousand kernel weight) and content of crude protein were elevated by 7.9% and 8.3%, respectively.”
e1“ENSIN fertilizer”
t0“maize grain”
Claim · model-written summary
ENSIN fertilization elevated maize crude protein content by 8.3%.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Both parameters TKW (thousand kernel weight) and content of crude protein were elevated by 7.9% and 8.3%, respectively.”
e1“ENSIN fertilizer”
t0“maize grain”
Claim · model-written summary
ENSIN fertilization increased maize protein production by 253 kg, or 31.9%.
- Treatment
- ENSIN fertilizere1
- Outcome
- Production of protein per hectaree0
- Direction
- increasede0
- Value
- 253 kge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Production of protein per hectare was increased by 253 kg, that is by 31.9%”
e1“ENSIN fertilizer”
t0“maize grain”
Claim · model-written summary
ENSIN fertilization had the highest nitrogen-use effectiveness, 9.8 kg grain per 1 kg nitrogen.
- Treatment
- ENSIN fertilizere1
- Outcome
- natural effectiveness of fertilizatione0
- Direction
- higheste0
- Value
- 9.8 kg of grain per 1 kg of applied nitrogene0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“natural effectiveness of fertilization achieved the highest value, namely 9.8 kg of grain per 1 kg of applied nitrogen”
e1“ENSIN fertilizer”
t0“maize grain”
Claim · model-written summary
ENSIN results were insignificantly better than split DASA at equal nitrogen and sulphur rates.
- Treatment
- fertilizer ENSINe1
- Outcome
- all examined parameterse0
- Direction
- insignificantly bettere0
- Comparator
- split application of DASA 26/13 fertilizere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the achieved results were insignificantly better in all examined parameters in comparison with the split application of DASA 26/13 fertilizer under the same rate of both nitrogen and sulphur”
e1“fertilizer ENSIN (contains inhibitors of nitrification) was applied in maize nutrition”
Claim · model-written summary
Adding sulphur to nitrogen significantly increased maize grain yield.
- Treatment
- Addition of sulphur to nitrogene0
- Outcome
- grain yielde0
- Direction
- increasede0
- Comparator
- nitrogene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Addition of sulphur to nitrogen as well as an addition of inhibitors + sulphur to nitrogen significantly increased only grain yield of maize.”
Claim · model-written summary
Adding inhibitors and sulphur to nitrogen significantly increased maize grain yield.
- Treatment
- addition of inhibitors + sulphur to nitrogene0
- Outcome
- grain yielde0
- Direction
- increasede0
- Comparator
- nitrogene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Addition of sulphur to nitrogen as well as an addition of inhibitors + sulphur to nitrogen significantly increased only grain yield of maize.”
Claim · model-written summary
Sulphur and inhibitor additions increased maize crude protein and thousand kernel weight without statistical significance.
- Treatment
- Addition of sulphur to nitrogene1
- Outcome
- Content of crude protein and TKWe0
- Direction
- increased insignificantlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Content of crude protein and TKW were increased insignificantly.”
e1“Addition of sulphur to nitrogen as well as an addition of inhibitors + sulphur to nitrogen”
e2“grain yield of maize”
Claim · model-written summary
Nitrogen fertilization effectiveness rose in the order nitrogen, nitrogen plus sulphur, then nitrogen plus sulphur plus inhibitors.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Natural effectiveness of nitrogen fertilization was increasing in accordance with raising yields of maize grain as follows: nitrogen < nitrogen + sulphur < nitrogen + sulphur + inhibitors.”
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