Abstract
Aquaporins play a major role in plant water uptake at both optimal and environmentally stressed conditions. However, the functional specificity of aquaporins under cold remains obscure. To get a better insight to the role of aquaporins in cold acclimation and freezing tolerance, we took an integrated approach of physiology, transcript profiling and cell biology in Arabidopsis thaliana. Cold acclimation resulted in specific upregulation of PIP1;4 and PIP2;5 aquaporin (plasma membrane intrinsic proteins) expression, and immunoblotting analysis confirmed the increase in amount of PIP2;5 protein and total amount of PIPs during cold acclimation, suggesting that PIP2;5 plays a major role in tackling the cold milieu. Although single mutants of pip1;4 and pip2;5 or their double mutant showed no phenotypic changes in freezing tolerance, they were more sensitive in root elongation and cell survival response under freezing stress conditions compared with the wild type. Consistently, a single mutation in either PIP1;4 or PIP2;5 altered the expression of a number of aquaporins both at the transcriptional and translational levels. Collectively, our results suggest that aquaporin members including PIP1;4 and PIP2;5 function in concert to regulate cold acclimation and freezing tolerance responses.
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Claims 13
Claim · model-written summary
Cold acclimation upregulated PIP1;4 expression.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Treatment
- Cold acclimatione0
- Gene
- PIP1;4e0
- Outcome
- PIP1;4 and PIP2;5 aquaporin (plasma membrane intrinsic proteins) expressione0
- Direction
- upregulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cold acclimation resulted in specific upregulation of PIP1;4 and PIP2;5 aquaporin (plasma membrane intrinsic proteins) expression”
t0“Arabidopsis thaliana”
Claim · model-written summary
Cold acclimation upregulated PIP2;5 expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Cold acclimatione0
- Gene
- PIP2;5e0
- Outcome
- PIP1;4 and PIP2;5 aquaporin (plasma membrane intrinsic proteins) expressione0
- Direction
- upregulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cold acclimation resulted in specific upregulation of PIP1;4 and PIP2;5 aquaporin (plasma membrane intrinsic proteins) expression”
t0“Arabidopsis thaliana”
Claim · model-written summary
Cold acclimation increased PIP2;5 protein abundance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- cold acclimatione0
- Gene
- PIP2;5 proteine0
- Outcome
- amount of PIP2;5 proteine0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“immunoblotting analysis confirmed the increase in amount of PIP2;5 protein and total amount of PIPs during cold acclimation”
t0“Arabidopsis thaliana”
Claim · model-written summary
Cold acclimation increased total PIP abundance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- cold acclimatione0
- Gene
- PIPse0
- Outcome
- total amount of PIPse0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increase in amount of PIP2;5 protein and total amount of PIPs during cold acclimation”
t0“Arabidopsis thaliana”
Claim · model-written summary
Single pip1;4 mutants showed no phenotypic change in freezing tolerance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- pip1;4e0
- Outcome
- freezing tolerancee0
- Direction
- no phenotypic changese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“single mutants of pip1;4 and pip2;5 or their double mutant showed no phenotypic changes in freezing tolerance”
t0“Arabidopsis thaliana”
Claim · model-written summary
Single pip2;5 mutants showed no phenotypic change in freezing tolerance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- pip2;5e0
- Outcome
- freezing tolerancee0
- Direction
- no phenotypic changese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“single mutants of pip1;4 and pip2;5 or their double mutant showed no phenotypic changes in freezing tolerance”
t0“Arabidopsis thaliana”
Claim · model-written summary
The pip1;4 pip2;5 double mutant showed no phenotypic change in freezing tolerance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- double mutante0
- Outcome
- freezing tolerancee0
- Direction
- no phenotypic changese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“single mutants of pip1;4 and pip2;5 or their double mutant showed no phenotypic changes in freezing tolerance”
t0“Arabidopsis thaliana”
Claim · model-written summary
The pip1;4 mutant had more sensitive root elongation and cell survival under freezing than wild type.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- pip1;4e1
- Treatment
- freezing stress conditionse0
- Outcome
- root elongation and cell survival responsee0
- Direction
- more sensitivee0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“they were more sensitive in root elongation and cell survival response under freezing stress conditions compared with the wild type”
e1“single mutants of pip1;4 and pip2;5 or their double mutant”
t0“Arabidopsis thaliana”
Claim · model-written summary
The pip2;5 mutant had more sensitive root elongation and cell survival under freezing than wild type.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- pip2;5e1
- Treatment
- freezing stress conditionse0
- Outcome
- root elongation and cell survival responsee0
- Direction
- more sensitivee0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“they were more sensitive in root elongation and cell survival response under freezing stress conditions compared with the wild type”
e1“single mutants of pip1;4 and pip2;5 or their double mutant”
t0“Arabidopsis thaliana”
Claim · model-written summary
The double mutant had more sensitive root elongation and cell survival under freezing than wild type.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- double mutante1
- Treatment
- freezing stress conditionse0
- Outcome
- root elongation and cell survival responsee0
- Direction
- more sensitivee0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“they were more sensitive in root elongation and cell survival response under freezing stress conditions compared with the wild type”
e1“single mutants of pip1;4 and pip2;5 or their double mutant”
t0“Arabidopsis thaliana”
Claim · model-written summary
PIP1;4 mutation altered other aquaporin expression at transcript and protein levels.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- PIP1;4e0
- Outcome
- expression of a number of aquaporinse0
- Qualifier
- both at the transcriptional and translational levelse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a single mutation in either PIP1;4 or PIP2;5 altered the expression of a number of aquaporins both at the transcriptional and translational levels”
t0“Arabidopsis thaliana”
Claim · model-written summary
PIP2;5 mutation altered other aquaporin expression at transcript and protein levels.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- PIP2;5e0
- Outcome
- expression of a number of aquaporinse0
- Qualifier
- both at the transcriptional and translational levelse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a single mutation in either PIP1;4 or PIP2;5 altered the expression of a number of aquaporins both at the transcriptional and translational levels”
t0“Arabidopsis thaliana”
Claim · model-written summary
The authors infer that PIP1;4 and PIP2;5 act together in cold acclimation and freezing tolerance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- PIP1;4 and PIP2;5e0
- Outcome
- cold acclimation and freezing tolerance responsese0
- Qualifier
- function in concerte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“aquaporin members including PIP1;4 and PIP2;5 function in concert to regulate cold acclimation and freezing tolerance responses”
t0“Arabidopsis thaliana”
The same questions in other papers
cold · quality: protein · Arabidopsis thaliana (thale cress)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
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18 papers in the same direction as this paper · 14 in the opposite direction
- no effect
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29 papers more in this group
See all 33 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.