Abstract
Micro propagation protocol from selected plants of Rosa hybrida using nodal segments as explant was standardized. Shoot cultures were initiated on MS medium supplemented with 6-benzyle adenine (BA, 2.5 μM). For shoot proliferation, BA and Kinetin (KIN) either alone or in combination with giberellic acid (GA3) were used. BA was found to be better for shoot proliferation and elongation as compared with KIN. Maximum shoot proliferation was observed on MS medium containing 10.0 μM BA in combination with 0.5 μM GA3. Indole 3-butyric acid (IBA) was found to be better auxin for induction of roots and maximum rooting frequency was observed on ¼ MS medium supplemented with 5.0 μM of IBA. Micro propagated plants showed 85 % survival during polyhouse and greenhouse conditions.
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Claims 6
Claim · model-written summary
A nodal segment micropropagation protocol for Rosa hybrida was standardized.
- Taxon
- Rosa hybrida
Rosa hybrid cultivare0 - Organ / stage
- nodal segmentse0
- Outcome
- Micro propagation protocole0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Micro propagation protocol from selected plants of Rosa hybrida using nodal segments as explant was standardized.”
Claim · model-written summary
BA outperformed KIN for shoot proliferation and elongation.
- Taxon
- Rosa hybrida L.Rosa hybrid cultivart0
- Treatment
- BAe0
- Outcome
- shoot proliferation and elongatione0
- Direction
- bettere0
- Comparator
- KINe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BA was found to be better for shoot proliferation and elongation as compared with KIN.”
t0“Rosa hybrida L.”
Claim · model-written summary
10.0 μM BA with 0.5 μM GA3 gave maximum shoot proliferation.
- Taxon
- Rosa hybrida L.Rosa hybrid cultivart0
- Treatment
- MS medium containing 10.0 μM BA in combination with 0.5 μM GA3e0
- Outcome
- Maximum shoot proliferatione0
- Direction
- Maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum shoot proliferation was observed on MS medium containing 10.0 μM BA in combination with 0.5 μM GA3.”
t0“Rosa hybrida L.”
Claim · model-written summary
IBA was the better auxin for root induction.
- Taxon
- Rosa hybrida L.Rosa hybrid cultivart0
- Treatment
- Indole 3-butyric acid (IBA)e0
- Outcome
- induction of rootse0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Indole 3-butyric acid (IBA) was found to be better auxin for induction of roots”
t0“Rosa hybrida L.”
Claim · model-written summary
Quarter strength MS medium with 5.0 μM IBA gave maximum rooting frequency.
- Taxon
- Rosa hybrida L.Rosa hybrid cultivart0
- Treatment
- ¼ MS medium supplemented with 5.0 μM of IBAe0
- Outcome
- rooting frequencye0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum rooting frequency was observed on ¼ MS medium supplemented with 5.0 μM of IBA.”
t0“Rosa hybrida L.”
Claim · model-written summary
Micropropagated Rosa plants showed 85% survival in polyhouse and greenhouse conditions.
- Taxon
- Rosa hybrida L.Rosa hybrid cultivart0
- Outcome
- survivale0
- Value
- 85 %e0
- Setting
- polyhouse and greenhouse conditionse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Micro propagated plants showed 85 % survival during polyhouse and greenhouse conditions.”
t0“Rosa hybrida L.”
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