Abstract
Several treatments were applied to the apple cv Gala to induce shoot regeneration. The influence of carbon source, combination and dosage of hormones, kind and age of the explants were evaluated. The leaves seemed to be the most competent for organogenesis. Both sorbitol and sucrose induced 50 % regeneration in leaves, while the internode sections were stimulated by the sorbitol. Glucose had a negative effect on regeneration except for the petioles. Good results were obtained with leaves in the presence of BAP 22.2 μM and NAA 1.34 μM, with optimal result from apical leaves (about 100 %). Organogenesis was strongly inhibited by TDZ, whereas the combination with BA had an intermediate effect on the explants. Molecular analysis through RAPDs showed that the regenerants were genetically stable.
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Claims 10
Claim · model-written summary
Leaves were the most competent explants for organogenesis.
- Taxon
- APPLE
Malus domesticat0 - Organ / stage
- leavese0
- Outcome
- most competent for organogenesise0
- Direction
- most competente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The leaves seemed to be the most competent for organogenesis”
t0“APPLE CULTIVAR GALA”
Claim · model-written summary
Sorbitol induced 50 % regeneration in leaves.
- Taxon
- APPLEMalus domesticat0
- Organ / stage
- leavese0
- Treatment
- sorbitole0
- Outcome
- regeneration in leavese0
- Direction
- inducede0
- Value
- 50 %e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both sorbitol and sucrose induced 50 % regeneration in leaves”
t0“APPLE CULTIVAR GALA”
Claim · model-written summary
Sucrose induced 50 % regeneration in leaves.
- Taxon
- APPLEMalus domesticat0
- Organ / stage
- leavese0
- Treatment
- sucrosee0
- Outcome
- regeneration in leavese0
- Direction
- inducede0
- Value
- 50 %e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both sorbitol and sucrose induced 50 % regeneration in leaves”
t0“APPLE CULTIVAR GALA”
Claim · model-written summary
Sorbitol stimulated regeneration of internode sections.
- Taxon
- APPLEMalus domesticat0
- Organ / stage
- internode sectionse0
- Treatment
- sorbitole0
- Outcome
- internode sections were stimulatede0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the internode sections were stimulated by the sorbitol”
t0“APPLE CULTIVAR GALA”
Claim · model-written summary
Glucose negatively affected regeneration except in petioles.
- Taxon
- APPLEMalus domesticat0
- Treatment
- Glucosee0
- Outcome
- regeneratione0
- Direction
- negative effecte0
- Qualifier
- except for the petiolese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glucose had a negative effect on regeneration except for the petioles”
t0“APPLE CULTIVAR GALA”
Claim · model-written summary
BAP 22.2 μM and NAA 1.34 μM gave good regeneration results with leaves.
- Taxon
- APPLEMalus domesticat0
- Organ / stage
- leavese0
- Treatment
- BAP 22.2 μM and NAA 1.34 μMe0
- Outcome
- Good resultse0
- Direction
- Goode0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Good results were obtained with leaves in the presence of BAP 22.2 μM and NAA 1.34 μM”
t0“APPLE CULTIVAR GALA”
Claim · model-written summary
Apical leaves gave about 100 % regeneration, the optimal result.
- Taxon
- APPLEMalus domesticat0
- Organ / stage
- apical leavese0
- Outcome
- optimal resulte0
- Direction
- optimale0
- Value
- about 100 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with optimal result from apical leaves (about 100 %)”
t0“APPLE CULTIVAR GALA”
Claim · model-written summary
TDZ strongly inhibited organogenesis.
- Taxon
- APPLEMalus domesticat0
- Treatment
- TDZe0
- Outcome
- Organogenesise0
- Direction
- strongly inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Organogenesis was strongly inhibited by TDZ”
t0“APPLE CULTIVAR GALA”
Claim · model-written summary
TDZ combined with BA had an intermediate effect on explants.
- Taxon
- APPLEMalus domesticat0
- Treatment
- combination with BAe0
- Outcome
- intermediate effect on the explantse0
- Direction
- intermediatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the combination with BA had an intermediate effect on the explants”
t0“APPLE CULTIVAR GALA”
Claim · model-written summary
RAPD analysis indicated that the regenerants were genetically stable.
- Taxon
- APPLEMalus domesticat0
- Organ / stage
- regenerantse0
- Outcome
- regenerants were genetically stablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Molecular analysis through RAPDs showed that the regenerants were genetically stable”
t0“APPLE CULTIVAR GALA”
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See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.