Abstract
The INDETERMINATE DOMAIN ( IDD ) family belongs to a group of plant-specific transcription factors that coordinates plant growth/development and immunity. However, the function and mode of action of IDDs during abiotic stress, such as salt, are poorly understood. We used idd4 transgenic lines and screened them under salt stress to find the involvement of IDD4 in salinity stress tolerance The genetic disruption of IDD4 increases salt-tolerance, characterized by sustained plant growth, improved Na + /K + ratio, and decreased stomatal density/aperture. Yet, IDD4 overexpressing plants were hypersensitive to salt-stress with an increase in stomatal density and pore size. Transcriptomic and ChIP-seq analyses revealed that IDD4 directly controls an important set of genes involved in abiotic stress/salinity responses. Interestingly, using anti-IDD4-pS73 antibody we discovered that IDD4 is specifically phosphorylated at serine-73 by MPK6 in vivo under salinity stress. Analysis of plants expressing the phospho-dead and phospho-mimicking IDD4 versions proved that phosphorylation of IDD4 plays a crucial role in plant transcriptional reprogramming of salt-stress genes. Altogether, we show that salt stress adaption involves MPK6 phosphorylation of IDD4 thereby regulating IDD4 DNA-binding and expression of target genes.
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Claims 10
Claim · model-written summary
IDD4 disruption increased salt tolerance.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- genetic disruption of IDD4e0
- Gene
- IDD4e0
- Outcome
- salt-tolerancee0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The genetic disruption of IDD4 increases salt-tolerance, characterized by sustained plant growth, improved Na + /K + ratio, and decreased stomatal density/aperture.”
t0“Arabidopsis”
Claim · model-written summary
IDD4 disruption sustained plant growth under salt stress.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- genetic disruption of IDD4e0
- Gene
- IDD4e0
- Outcome
- plant growthe0
- Direction
- sustainede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The genetic disruption of IDD4 increases salt-tolerance, characterized by sustained plant growth, improved Na + /K + ratio, and decreased stomatal density/aperture.”
t0“Arabidopsis”
Claim · model-written summary
IDD4 disruption improved the Na to K ratio.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- genetic disruption of IDD4e0
- Outcome
- Na + /K + ratioe0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The genetic disruption of IDD4 increases salt-tolerance, characterized by sustained plant growth, improved Na + /K + ratio, and decreased stomatal density/aperture.”
t0“Arabidopsis”
Claim · model-written summary
IDD4 disruption decreased stomatal density and aperture.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- genetic disruption of IDD4e0
- Outcome
- stomatal density/aperturee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The genetic disruption of IDD4 increases salt-tolerance, characterized by sustained plant growth, improved Na + /K + ratio, and decreased stomatal density/aperture.”
t0“Arabidopsis”
Claim · model-written summary
IDD4 overexpression caused salt hypersensitivity.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- IDD4 overexpressinge0
- Outcome
- hypersensitive to salt-stresse0
- Direction
- hypersensitivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IDD4 overexpressing plants were hypersensitive to salt-stress with an increase in stomatal density and pore size.”
t0“Arabidopsis”
Claim · model-written summary
IDD4 overexpression increased stomatal density and pore size.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- IDD4 overexpressinge0
- Outcome
- stomatal density and pore sizee0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IDD4 overexpressing plants were hypersensitive to salt-stress with an increase in stomatal density and pore size.”
t0“Arabidopsis”
Claim · model-written summary
IDD4 directly controlled genes involved in salinity responses.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- IDD4e0
- Outcome
- genes involved in abiotic stress/salinity responsese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IDD4 directly controls an important set of genes involved in abiotic stress/salinity responses.”
t0“Arabidopsis”
Claim · model-written summary
MPK6 phosphorylated IDD4 at serine-73 in vivo under salinity stress.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- salinity stresse0
- Gene
- IDD4e0
- Outcome
- phosphorylated at serine-73 by MPK6e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IDD4 is specifically phosphorylated at serine-73 by MPK6 in vivo under salinity stress.”
t0“Arabidopsis”
Claim · model-written summary
IDD4 phosphorylation contributed to transcriptional reprogramming of salt stress genes.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- IDD4e0
- Outcome
- plant transcriptional reprogramming of salt-stress genese0
- Qualifier
- phosphorylation of IDD4e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“phosphorylation of IDD4 plays a crucial role in plant transcriptional reprogramming of salt-stress genes.”
t0“Arabidopsis”
Claim · model-written summary
MPK6 phosphorylation of IDD4 regulated its DNA binding and target gene expression.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- IDD4e0
- Outcome
- IDD4 DNA-binding and expression of target genese0
- Qualifier
- MPK6 phosphorylation of IDD4e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“salt stress adaption involves MPK6 phosphorylation of IDD4 thereby regulating IDD4 DNA-binding and expression of target genes.”
t0“Arabidopsis”
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